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Literature summary for 2.7.9.3 extracted from

  • Yeh, M.S.; Huang, C.J.; Guo, C.H.; Liu, K.F.; Tsai, I.H.; Cheng, W.
    Identification and cloning of a selenophosphate synthetase (SPS) from tiger shrimp, Penaeus monodon, and its transcription in relation to molt stages and following pathogen infection (2012), Dev. Comp. Immunol., 36, 21-30.
    View publication on PubMed
Search for more literature for 2.7.9.3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Penaeus monodon

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45500
-
 x * 45500, calculated from amino acid sequence Penaeus monodon

Organism

Organism UniProt Comment Textmining
Penaeus monodon F5CBP8 tiger shrimp
-

Source Tissue

Source Tissue Comment Organism Textmining
hemocyte
-
 Penaeus monodon
-
hepatopancreas
-
 Penaeus monodon
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + selenide + H2O
-
 Penaeus monodon AMP + selenophosphate + phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 45500, calculated from amino acid sequence Penaeus monodon

Synonyms

Synonyms Comment Organism
selenophosphate synthetase
-
 Penaeus monodon
SPS
-
 Penaeus monodon

pI Value

Organism Comment pI Value Maximum pI Value
Penaeus monodon calculated from amino acid sequence
-
 4.9

Expression

Organism Comment Expression
Penaeus monodon SPS mRNA expression in hemocytes of shrimp injected with white spot syndrome virus significantly decreases from 3 to 48 h down
Penaeus monodon enzyme expression by hemocytes significantly increases in stage C, and then gradually decreases until stage A. The relative enzyme expression (compared to enzyme expression in shrimp injected with saline) of shrimp injected with white spot syndrome virus after 3, 6, 12, 24 and 48 h increases by 7.0, 4.4, 2.0, 1.6, and 1.8fold, respectively up

General Information

General Information Comment Organism
physiological function the cloned enzyme in hemocytes plays a role in viability by renewing hemocytes and antioxidative stress response for new exoskeleton synthesis during the molt cycle of shrimp Penaeus monodon