Literature summary for 2.7.9.3 extracted from

Wang, K.T.; Wang, J.; Li, L.F.; Su, X.D.
Crystal structures of catalytic intermediates of human selenophosphate synthetase 1 (2009), J. Mol. Biol., 390, 747-759.

Search for more literature for 2.7.9.3

Cloned(Commentary)

Cloned (Comment)	Organism
into the vector pET28a for expression in Escherichia coli Rosetta DE3 cells	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
the crystal structures of selenophosphate synthetase 1 and of selenophosphate synthetase 1 complexed with AMPCP and K+ are solved to a resolution of 2.0 and 1.9 A, respectively	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
K+	-	Homo sapiens
Mg2+	-	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + selenide + H2O	Homo sapiens	-	AMP + selenophosphate + phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P49903	-	-

Purification (Commentary)

Purification (Comment)	Organism
on a Ni-chelating column, the His-tag is removed by thrombin digestion, further purification by gel-filtration	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + selenide + H2O	-	Homo sapiens	AMP + selenophosphate + phosphate	-	?

Subunits

Subunits	Comment	Organism
homodimer	-	Homo sapiens

Synonyms

Synonyms	Comment	Organism
hSPS1	-	Homo sapiens
selenophosphate synthetase 1	-	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Homo sapiens

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)