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Literature summary for 2.7.9.3 extracted from
- Selenocysteine incorporation in kinetoplastid: selenophosphate synthetase (SELD) from Leishmania major and Trypanosoma brucei (2008), Mol. Biochem. Parasitol., 162, 165-171.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene selD, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strain BL21(DE3), and functional complementation of Escherichia coli SelD deletion strain WL400 | Trypanosoma brucei |
| gene selD, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strain BL21(DE3), and functional complementation of Escherichia coli SelD deletion strain WL400 | Leishmania major |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C42A | site-directed mutagenesis | Trypanosoma brucei |
| C46A | site-directed mutagenesis | Leishmania major |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| kinetoplast | - |
Trypanosoma brucei | 20023 | - |
| kinetoplast | - |
Leishmania major | 20023 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Trypanosoma brucei |  |
| Mg2+ | - |
Leishmania major | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42700 | - |
2 * 42700, about, sequence calculation, 2 * 45000, recombinant SelD, SDS-PAGE | Leishmania major |
| 43000 | - |
2 * 43000, about, sequence calculation, 2 * 45000, recombinant SelD, SDS-PAGE | Trypanosoma brucei |
| 45000 | - |
2 * 42700, about, sequence calculation, 2 * 45000, recombinant SelD, SDS-PAGE | Leishmania major |
| 45000 | - |
2 * 43000, about, sequence calculation, 2 * 45000, recombinant SelD, SDS-PAGE | Trypanosoma brucei |
| 100000 | - |
about, gel filtration | Trypanosoma brucei |
| 100000 | - |
about, gel filtration | Leishmania major |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + selenide | Trypanosoma brucei | selenophosphate synthetase produces the biologically active selenium donor compound, monoselenophosphate, from ATP and selenide, for the synthesis of selenocysteine | AMP + selenophosphate + phosphate | - |
? | |
| ATP + selenide | Leishmania major | selenophosphate synthetase produces the biologically active selenium donor compound, monoselenophosphate, from ATP and selenide, for the synthesis of selenocysteine | AMP + selenophosphate + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Leishmania major | Q4Q0M0 | gene selD | - |
| Trypanosoma brucei | - |
gene selD | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant SelD from Escherichia coli strain BL21(DE3) to homogeneity | Trypanosoma brucei |
| recombinant SelD from Escherichia coli strain BL21(DE3) to homogeneity | Leishmania major |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| epimastigote | cell culture | Trypanosoma brucei | - |
| epimastigote | cell culture | Leishmania major | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + selenide | - |
Trypanosoma brucei | AMP + selenophosphate + phosphate | - |
? | |
| ATP + selenide | - |
Leishmania major | AMP + selenophosphate + phosphate | - |
? | |
| ATP + selenide | selenophosphate synthetase produces the biologically active selenium donor compound, monoselenophosphate, from ATP and selenide, for the synthesis of selenocysteine | Trypanosoma brucei | AMP + selenophosphate + phosphate | - |
? | |
| ATP + selenide | selenophosphate synthetase produces the biologically active selenium donor compound, monoselenophosphate, from ATP and selenide, for the synthesis of selenocysteine | Leishmania major | AMP + selenophosphate + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 42700, about, sequence calculation, 2 * 45000, recombinant SelD, SDS-PAGE | Leishmania major |
| dimer | 2 * 43000, about, sequence calculation, 2 * 45000, recombinant SelD, SDS-PAGE | Trypanosoma brucei |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SelD | - |
Trypanosoma brucei |
| SelD | - |
Leishmania major |
| selenophosphate synthetase | - |
Trypanosoma brucei |
| selenophosphate synthetase | - |
Leishmania major |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Trypanosoma brucei |
| 30 | - |
assay at | Leishmania major |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
assay at | Trypanosoma brucei |
| 7.2 | - |
assay at | Leishmania major |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Trypanosoma brucei | |
| ATP | - |
Leishmania major | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Leishmania major | isoelectric focusing, recombinant enzyme | - |
6.05 |
| Trypanosoma brucei | isoelectric focusing, recombinant enzyme | - |
6.34 |
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