Literature summary for 2.7.9.3 extracted from

Matsumoto, E.; Sekine, S.; Akasaka, R.; Otta, Y.; Katsura, K.; Inoue, M.; Kaminishi, T.; Terada, T.; Shirouzu, M.; Yokoyama, S.
Structure of an N-terminally truncated selenophosphate synthetase from Aquifex aeolicus (2008), Acta Crystallogr. Sect. F, F64, 453-458.

Cloned(Commentary)

Cloned (Comment)	Organism
expression of truncated mutant enzyme SPS-DELTAN in Escherichia coli	Aquifex aeolicus

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant truncated mutant enzyme SPS-DELTAN, mixing of 0.001 ml of protein solution, containing 9.9 mg/ml protein in 20 mM Tris-HCl, pH 8.0, 2 mM dithiothreitol, and 150 mM NaCl, with 0.001 ml of a reservoir solution containing 100 mM Na HEPES, pH 7.1, 50% 2-methyl-2,4-pentanediol, 200 mM ammonium phosphate, and 50 mM ammonium sulfate and equilibrating this mixture against 0.5 ml reservoir solution at 20°C, X-ray diffraction structure determination and analysis at 2.0 A resolution, hexameric crystal structure	Aquifex aeolicus

Crystallization (Comment)

Organism

purified recombinant truncated mutant enzyme SPS-DELTAN, mixing of 0.001 ml of protein solution, containing 9.9 mg/ml protein in 20 mM Tris-HCl, pH 8.0, 2 mM dithiothreitol, and 150 mM NaCl, with 0.001 ml of a reservoir solution containing 100 mM Na HEPES, pH 7.1, 50% 2-methyl-2,4-pentanediol, 200 mM ammonium phosphate, and 50 mM ammonium sulfate and equilibrating this mixture against 0.5 ml reservoir solution at 20°C, X-ray diffraction structure determination and analysis at 2.0 A resolution, hexameric crystal structure

Aquifex aeolicus

Protein Variants

Protein Variants	Comment	Organism
additional information	construction of an N-terminally truncated SPS mutant lacking 25 amino acid residues, i.e SPS-DELTAN	Aquifex aeolicus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
63000	-	about, analytical ultracentrifugation	Aquifex aeolicus
68200	-	about, sequence calculation	Aquifex aeolicus
204000	-	crystal structure	Aquifex aeolicus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + selenide	Aquifex aeolicus	selenophosphate synthetase catalyzes the activation of selenide with ATP to synthesize selenophosphate, the reactive selenium donor for biosyntheses of both the 21st amino acid selenocysteine and 2-selenouridine nucleotides in tRNA anticodons	AMP + selenophosphate + phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Aquifex aeolicus	O67139	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant truncated mutant enzyme SPS-DELTAN from Escherichia coli by heat treatment at 70°C for 30 min, anion exchange and hydrophobic interaction chromatography, followed by another step of anion exchange chromatography and gel filtration	Aquifex aeolicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + selenide	selenophosphate synthetase catalyzes the activation of selenide with ATP to synthesize selenophosphate, the reactive selenium donor for biosyntheses of both the 21st amino acid selenocysteine and 2-selenouridine nucleotides in tRNA anticodons	Aquifex aeolicus	AMP + selenophosphate + phosphate	-	?
ATP + selenide	the phosphate moiety of selenophosphate is derived from the ATP gamma-phosphate, while the orthophosphate is from the beta-phosphate	Aquifex aeolicus	AMP + selenophosphate + phosphate	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 31000-34000, about, sequence calculation and analytical ultracentrifugation	Aquifex aeolicus
hexamer	6 * 31000-34000, about, crystal structure	Aquifex aeolicus
More	SPS is a two-domain alpha/beta protein, with domain folds that are homologous to those of PurM superfamily proteins	Aquifex aeolicus

Synonyms

Synonyms	Comment	Organism
selenide water dikinase	-	Aquifex aeolicus
selenophosphate synthetase	-	Aquifex aeolicus
SPS	-	Aquifex aeolicus

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Aquifex aeolicus