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Literature summary for 2.7.9.3 extracted from
- Catalytic properties of selenophosphate synthetases: comparison of the selenocysteine-containing enzyme from Haemophilus influenzae with the corresponding cysteine-containing enzyme from Escherichia coli (1999), Proc. Natl. Acad. Sci. USA, 96, 44-48.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli strain BL21 | Haemophilus influenzae |
General Stability
| General Stability | Organism |
|---|---|
| concentration-dependent inactivation | Haemophilus influenzae |
| concentration-dependent inactivation | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| H2O2 | 62% inhibition at 10 mM | Escherichia coli |  |
| H2O2 | 53% inhibition at 5 mM | Haemophilus influenzae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
selenide | pH 7.8, 37°C | Escherichia coli | |
| 0.025 | - |
selenide | pH 7.8, 37°C | Haemophilus influenzae | |
| 0.9 | - |
ATP | pH 7.8, 37°C | Escherichia coli | |
| 1.3 | - |
ATP | pH 7.8, 37°C | Haemophilus influenzae | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
gene selD | - |
| Haemophilus influenzae | - |
gene selD | - |
| Haemophilus influenzae | - |
selenocysteine containing enzyme | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant from Escherichia coli, to homogeneity | Haemophilus influenzae |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + selenide + H2O = AMP + selenophosphate + phosphate | enzyme contains a selenocysteine residue at the active site | Haemophilus influenzae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 16 | - |
- |
Haemophilus influenzae |
| 29 | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + selenide + H2O | - |
Haemophilus influenzae | AMP + selenophosphate + phosphate | - |
? | |
| ATP + selenide + H2O | - |
Escherichia coli | AMP + selenophosphate + phosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Haemophilus influenzae |
| 37 | - |
assay at | Escherichia coli |
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Release 2023.1 (January 2023)
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