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Literature summary for 2.7.9.3 extracted from

  • Low, S.C.; Harney, J.W.; Berry, M.J.
    Cloning and functional characterization of human selenophosphate synthetase, an essential component of selenoprotein synthesis (1995), J. Biol. Chem., 270, 21659-21664.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
gene selD, DNA sequence determination and analysis, subcloning and transient expression in enzyme deficient Escherichia coli resulting in poor complemetation of the bacteria by the human gene, and expression in mammalian HtTA cells together with human type I iodothyronine 5'-deiodinase and Xenopus tRNASeC Homo sapiens

Protein Variants

Protein Variants Comment Organism
G269C site-directed mutagenesis, reduced activity, slightly reduced ATP binding Homo sapiens
G271R site-directed mutagenesis, reduced activity, slightly reduced ATP binding Homo sapiens
G274A site-directed mutagenesis, reduced activity and ATP binding Homo sapiens
G274D site-directed mutagenesis, reduced activity, no ATP binding Homo sapiens
G274V site-directed mutagenesis, no activity and ATP binding Homo sapiens
H275N site-directed mutagenesis, reduced activity, no ATP binding Homo sapiens
H275Y site-directed mutagenesis, increased catalytic activity and ATP binding Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
8-azido-ATP competitive to ATP Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P49903 gene selD
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + selenide + H2O
-
 Homo sapiens AMP + selenophosphate + phosphate
-
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