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Literature summary for 2.7.9.2 extracted from

  • Chen, X.; Li, M.; Zhou, L.; Shen, W.; Algasan, G.; Fan, Y.; Wang, Z.
    Metabolic engineering of Escherichia coli for improving shikimate synthesis from glucose (2014), Biores. Technol., 166, 64-71.
    View publication on PubMed
Search for more literature for 2.7.9.2

Cloned(Commentary)

Cloned (Comment) Organism
gene ppsA, subcloning in Escherichia coli strain JM19, recombinant overexpression in Escherichia coli strain B0013, coexpression with genes aroG and tktA Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information three critical enzymes of mutated 3-deoxy-D-arabinoheptulosonate-7-phosphate (DAHP) synthase (encoded by aroGfbr), PEP synthase (encoded by ppsA), and transketolase A (encoded by tktA) are modularly overexpressed in Escherichia coli and the resulting recombinant strain produces increased levels of shikimate in an optimized fed-batch fermentation process, overview Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + pyruvate + H2O Escherichia coli
-
 AMP + phosphoenolpyruvate + phosphate
-
 ir

Organism

Organism UniProt Comment Textmining
Escherichia coli P23538 B0013, gene ppsA
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + pyruvate + H2O
-
 Escherichia coli AMP + phosphoenolpyruvate + phosphate
-
 ir

Synonyms

Synonyms Comment Organism
PEP synthase
-
 Escherichia coli
phosphoenolpyruvate synthase
-
 Escherichia coli
PpsA
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Escherichia coli

General Information

General Information Comment Organism
metabolism phosphoenolpyruvate produced by the enzyme is required in the shikimate biosynthesis, pathway overview. The expression levels of the enzyme transketolase I (encoded by tktA) and phosphoenolpyruvate synthase (encoded by ppsA) have a critical impact on the phosphoenolpyruvate and erythrose-4-phosphate availability, which are important determinants for in vivo 3-deoxy-D-arabinoheptulosonate-7-phosphate synthase activity Escherichia coli