Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not affected by Na+ | Pyrococcus furiosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Pyrococcus furiosus | |
0.11 | - |
pyruvate | pH 8.4, 80°C | Pyrococcus furiosus | |
0.39 | - |
ATP | pH 8.4, 80°C | Pyrococcus furiosus | |
0.4 | - |
phosphoenolpyruvate | pH 8.4, 80°C | Pyrococcus furiosus | |
1 | - |
AMP | pH 8.4, 80°C | Pyrococcus furiosus | |
38.4 | - |
phosphate | pH 8.4, 80°C | Pyrococcus furiosus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | 5% of total cytoplasmic protein | Pyrococcus furiosus | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | enzyme contains 1 Ca atom per subunit | Pyrococcus furiosus | |
Co2+ | requires divalent cations in the forward reaction, 93% of the activity with Mg2+ | Pyrococcus furiosus | |
Iron | 0.1-0.4 g-atoms per subunit, no functional part of the enzyme | Pyrococcus furiosus | |
K+ | 50% increased activity at 100 mM | Pyrococcus furiosus | |
Mg2+ | slightly inhibitory above 10 mM | Pyrococcus furiosus | |
Mg2+ | requires divalent cations in the forward reaction, Mg2+ is most effective | Pyrococcus furiosus | |
Mn2+ | requires divalent cations in the forward reaction, 95% of the activity with Mg2+ | Pyrococcus furiosus | |
additional information | Ca2+ and Sr2+ cannot substitute for Mg2+ in the catalytic reaction | Pyrococcus furiosus | |
additional information | not affected by Na+ | Pyrococcus furiosus | |
NH4+ | 48% increased activity at 100 mM | Pyrococcus furiosus | |
Ni2+ | requires divalent cations in the forward reaction, 15% of the activity with Mg2+ | Pyrococcus furiosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
92000 | - |
8 * 92000, SDS-PAGE, 30% of the enzyme is purified as a large inactive complex of about 1640 kDa | Pyrococcus furiosus |
690000 | - |
gel filtration | Pyrococcus furiosus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyruvate + H2O | Pyrococcus furiosus | involved in gluconeogenesis | AMP + phosphoenolpyruvate + phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | - |
hyperthermophilic archaeon | - |
Purification (Comment) | Organism |
---|---|
19.4fold | Pyrococcus furiosus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
14.9 | - |
purified enzyme | Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2-ketobutyrate + H2O | 8% of the activity with pyruvate | Pyrococcus furiosus | ? | - |
? | |
ATP + 2-oxoglutarate + H2O | 2% of the activity with pyruvate | Pyrococcus furiosus | ? | - |
? | |
ATP + pyruvate + H2O | absolutely specific for ATP, highly specific for pyruvate | Pyrococcus furiosus | AMP + phosphoenolpyruvate + phosphate | - |
r | |
ATP + pyruvate + H2O | phosphorylated enzyme as an intermediate | Pyrococcus furiosus | AMP + phosphoenolpyruvate + phosphate | - |
r | |
ATP + pyruvate + H2O | equilibrium lies far to the side of phosphoenolpyruvate formation | Pyrococcus furiosus | AMP + phosphoenolpyruvate + phosphate | - |
r | |
ATP + pyruvate + H2O | involved in gluconeogenesis | Pyrococcus furiosus | AMP + phosphoenolpyruvate + phosphate | - |
r | |
additional information | no activity with 2-keto-3-methylvalerate, 2-ketoisocaproate, 2-ketomalonate, phenylpyruvate, hydroxyphenylpyruvate, imidazolpyruvate, and indole-3-pyruvate | Pyrococcus furiosus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | 8 * 92000, SDS-PAGE, 30% of the enzyme is purified as a large inactive complex of about 1640 kDa | Pyrococcus furiosus |
Synonyms | Comment | Organism |
---|---|---|
PpsA | - |
Pyrococcus furiosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
at pH 8.4 | Pyrococcus furiosus |
90 | - |
phosphoenolpyruvate formation | Pyrococcus furiosus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 90 | optimum at 90°C, 0.2% of the optimal activity at 30°C | Pyrococcus furiosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
t1/2: 9 h | Pyrococcus furiosus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.7 | - |
AMP | pH 8.4, 80°C | Pyrococcus furiosus | |
11.9 | - |
phosphate | pH 8.4, 80°C | Pyrococcus furiosus | |
12.6 | - |
phosphoenolpyruvate | pH 8.4, 80°C | Pyrococcus furiosus | |
1330 | - |
ATP | pH 8.4, 80°C | Pyrococcus furiosus | |
1570 | - |
pyruvate | pH 8.4, 80°C | Pyrococcus furiosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
at 80°C | Pyrococcus furiosus |
7.5 | - |
pyruvate formation | Pyrococcus furiosus |
9 | - |
at 80°C | Pyrococcus furiosus |
9 | - |
phosphoenolpyruvate formation | Pyrococcus furiosus |