Literature summary for 2.7.9.2 extracted from

Jakeman, D.L.; Evans, J.N.S.
Overexpression, purification, and use of phosphoenol pyruvate synthetase in the synthesis of PEP analogs (1998), Bioorg. Chem., 26, 245-253.

No PubMed abstract available

Search for more literature for 2.7.9.2

Cloned(Commentary)

Cloned (Comment)	Organism
gene ppsA, overexpression in strain BL21 (DE3)	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + pyruvate + H2O	Escherichia coli	involved in gluconeogenesis	AMP + phosphoenolpyruvate + phosphate	-	r

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P23538	gene ppsA	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme from overexpression, purification in a single chromatographic step	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + 3-fluoropyruvate + H2O	stereochemically specific reaction	Escherichia coli	AMP + (Z)-fluorophosphoenolpyruvate + phosphate	-	r
ATP + 3-fluoropyruvate + H2O	10times slower reaction rate than with pyruvate	Escherichia coli	AMP + (Z)-fluorophosphoenolpyruvate + phosphate	-	r
ATP + pyruvate + H2O	-	Escherichia coli	AMP + phosphoenolpyruvate + phosphate	-	r
ATP + pyruvate + H2O	involved in gluconeogenesis	Escherichia coli	AMP + phosphoenolpyruvate + phosphate	-	r

Synonyms

Synonyms	Comment	Organism
phosphoenol pyruvate synthetase	-	Escherichia coli

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.8	-	pyruvate formation	Escherichia coli
8.4	-	phosphoenolpyruvate formation	Escherichia coli

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Release 2023.1 (January 2023)