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Literature summary for 2.7.9.1 extracted from

  • Minges, A.; Hoeppner, A.; Groth, G.
    Trapped intermediate state of plant pyruvate phosphate dikinase indicates substeps in catalytic swiveling domain mechanism (2017), Protein Sci., 26, 1667-1673 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.9.1

Cloned(Commentary)

Cloned (Comment) Organism
codon-optimized coding regions of PPDK from Flaveria trinervia stripped of the chloroplast transport sequence are cloned into the multiple cloning site of a pET-16b vector (Novagen) containing a His10 tag and coding for a Tobacco Etch Virus protease cleavage site. Escherichia coli BL21 (DE3) cells are transformed with this plasmid Flaveria trinervia

Crystallization (Commentary)

Crystallization (Comment) Organism
crystals of the FtPPDK-pyruvate-ADP complex are obtained using the sitting drop vapor diffusion method at 21°C Flaveria trinervia

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + pyruvate + phosphate Flaveria trinervia essential enzyme of C4 photosynthetic pathway AMP + phosphoenolpyruvate + diphosphate
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 ?

Organism

Organism UniProt Comment Textmining
Flaveria trinervia P22221
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Flaveria trinervia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + pyruvate + phosphate
-
 Flaveria trinervia AMP + phosphoenolpyruvate + diphosphate
-
 ?
ATP + pyruvate + phosphate essential enzyme of C4 photosynthetic pathway Flaveria trinervia AMP + phosphoenolpyruvate + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
PPDK
-
 Flaveria trinervia

General Information

General Information Comment Organism
physiological function essential enzyme of C4 photosynthetic pathway Flaveria trinervia