Literature summary for 2.7.9.1 extracted from

Gonzalez-Marcano, E.; Mijares, A.; Quinones, W.; Caceres, A.; Concepcion, J.L.
Post-translational modification of the pyruvate phosphate dikinase from Trypanosoma cruzi (2014), Parasitol. Int., 63, 80-86.

Search for more literature for 2.7.9.1

Cloned(Commentary)

Cloned (Comment)	Organism
gene ppdk, recombinant expression of His-tagged enzyme in Escherichia coli strain TG-1	Trypanosoma cruzi

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
glycosome	the main part of the 100 kDa enzyme form of PPDK is soluble	Trypanosoma cruzi	20015	-
glycosome membrane	the 75 kDa PPDK enzyme form is located peripherally at the glycosomal membrane	Trypanosoma cruzi	46860	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
75000	-	-	Trypanosoma cruzi
100000	-	-	Trypanosoma cruzi

Organism

Organism	UniProt	Comment	Textmining
Trypanosoma cruzi	Q9GN79	isolated from a patient with Chagas disease in acute phase	-
Trypanosoma cruzi EP	Q9GN79	isolated from a patient with Chagas disease in acute phase	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
additional information	glucose starvation and the concomitant switch of the metabolism to amino acid catabolism may play a role in the post-translational processing of the PPDK. Either one or both of the processes, phosphorylation and proteolytic cleavage of PPDK, result in inactivation of the enzyme	Trypanosoma cruzi
phosphoprotein	the 75 kDa enzyme form is phosphorylated at the highly conserved Thr481 residue. Phosphorlation leads to inactivation. The 100 kDa enzyme form is not phosphorylated	Trypanosoma cruzi
proteolytic modification	the enzyme is post-translationally modified by proteolytic cleavage, which leads to inactivation	Trypanosoma cruzi

Purification (Commentary)

Purification (Comment)	Organism
purification of glycosomes from epimastigotes and subcellular fractionation, recombinant His-tagged enzyme from Escherichia coli strain TG-1 by nickel affinity chromatography	Trypanosoma cruzi

Source Tissue

Source Tissue	Comment	Organism	Textmining
epimastigote	during growth of epimastigotes in batch culture, an increase in the level of the 75 kDa enzyme form and a decrease of the 100 kDa enzyme form are observed. Expression of the 100 kDa PPDK slightly decreases, when the culture reaches the stationary phase, but its specific activity decreases about 3.25times to an almost undetectable level, and the phosphorylated 75 kDa PPDK signal increases significantly	Trypanosoma cruzi	-
additional information	both PPDK enzyme forms are found in all life-cycle stages of the parasite	Trypanosoma cruzi	-

Subunits

Subunits	Comment	Organism
?	x * 75000, short enzyme form, SDS-PAGE, x * 100000, long enzyme form, SDS-PAGE	Trypanosoma cruzi

Synonyms

Synonyms	Comment	Organism
pyruvate phosphate dikinase	-	Trypanosoma cruzi

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Trypanosoma cruzi

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.3	-	assay at	Trypanosoma cruzi

General Information

General Information	Comment	Organism
physiological function	pyruvate phosphate dikinase is an auxiliary enzyme of glycolysis located in the glycosomes	Trypanosoma cruzi

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Release 2023.1 (January 2023)