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Literature summary for 2.7.9.1 extracted from

  • Deramchia, K.; Morand, P.; Biran, M.; Millerioux, Y.; Mazet, M.; Wargnies, M.; Franconi, J.M.; Bringaud, F.
    Contribution of pyruvate phosphate dikinase in the maintenance of the glycosomal ATP/ADP balance in the Trypanosoma brucei procyclic form (2014), J. Biol. Chem., 289, 17365-17378.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.9.1

Protein Variants

Protein Variants Comment Organism
additional information construction of a PPDK deletion mutant, DELTAppdk, and a DELTAppdk/DELTApepck double deletion mutant Trypanosoma brucei

Localization

Localization Comment Organism GeneOntology No. Textmining
glycosome
-
 Trypanosoma brucei 20015
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Trypanosoma brucei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + pyruvate + phosphate Trypanosoma brucei PPDK functions in the glycolytic direction with production of ATP in the glycosomes AMP + phosphoenolpyruvate + diphosphate
-
 r
ATP + pyruvate + phosphate Trypanosoma brucei EATRO1125.T7T and 427 90-13 PPDK functions in the glycolytic direction with production of ATP in the glycosomes AMP + phosphoenolpyruvate + diphosphate
-
 r

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei O76283
-
-
Trypanosoma brucei EATRO1125.T7T and 427 90-13 O76283
-
-

Source Tissue

Source Tissue Comment Organism Textmining
bloodstream form 427 90-13 Trypanosoma brucei
-
procyclic form EATRO1125.T7T Trypanosoma brucei
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + pyruvate + phosphate
-
 Trypanosoma brucei AMP + phosphoenolpyruvate + diphosphate
-
 r
ATP + pyruvate + phosphate PPDK functions in the glycolytic direction with production of ATP in the glycosomes Trypanosoma brucei AMP + phosphoenolpyruvate + diphosphate
-
 r
ATP + pyruvate + phosphate
-
 Trypanosoma brucei EATRO1125.T7T and 427 90-13 AMP + phosphoenolpyruvate + diphosphate
-
 r
ATP + pyruvate + phosphate PPDK functions in the glycolytic direction with production of ATP in the glycosomes Trypanosoma brucei EATRO1125.T7T and 427 90-13 AMP + phosphoenolpyruvate + diphosphate
-
 r

Synonyms

Synonyms Comment Organism
PPDK
-
 Trypanosoma brucei
pyruvate phosphate dikinase
-
 Trypanosoma brucei

Cofactor

Cofactor Comment Organism Structure
AMP
-
 Trypanosoma brucei
ATP
-
 Trypanosoma brucei

General Information

General Information Comment Organism
malfunction deletion of the trypanosomal PPDK gene affects glycolysis. The rate of acetate production from glucose is 30% reduced in the DELTAppdk mutant, whereas threonine-derived acetate production is not affected. The DELTAppdk/DELTApepck double mutant, also lacking phosphoenolpyruvate carboxykinase, PEPCK, is incubated in glucose as the only carbon source and shows a 3.8fold reduction of the glycolytic rate compared with the DELTApepck mutant, as a consequence of the imbalanced glycosomal ATP/ADP ratio. Expressing the glycosomal phosphoglycerate kinase (PGKC) in the DELTAppdk/DELTApepck cell line restores the glycolytic flux, but expression of PGKC is lethal for procyclic trypanosomes, as a consequence of ATP depletion, due to glycosomal relocation of cytosolic ATP production. Comparison of glucose metabolism regulation of wild-type and mutant enzymes, the glucose metabolism is strongly impaired in the DELTAppdk/DELTApepck mutant, overview Trypanosoma brucei
physiological function pyruvate phosphate dikinase (PPDK) is a diphosphate-dependent enzyme, which reversibly catalyzes conversion of phosphoenolpyruvate, diphosphate, and AMP into pyruvate, phosphate, and ATP. In trypanosomes, enzyme PPDK works in the glycolytic direction and participates in the maintenance of the glycosomal ATP/ADP balance. The glycosomal PPDK provides a metabolic flexibility by producing 2 ATP per phosphoenolpyruvate consumed. Role of enzyme PPDK in acetate production Trypanosoma brucei