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Literature summary for 2.7.9.1 extracted from

  • Zuniga-Ripa, A.; Barbier, T.; Conde-Alvarez, R.; Martinez-Gomez, E.; Palacios-Chaves, L.; Gil-Ramirez, Y.; Grillo, M.J.; Letesson, J.J.; Iriarte, M.; Moriyon, I.
    Brucella abortus depends on pyruvate phosphate dikinase and malic enzyme but not on Fbp and GlpX fructose-1,6-bisphosphatases for full virulence in laboratory models (2014), J. Bacteriol., 196, 3045-3057.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.9.1

Cloned(Commentary)

Cloned (Comment) Organism
gene ppdK, expression analysis Brucella abortus

Protein Variants

Protein Variants Comment Organism
additional information construction of an enzyme deletion mutant DELTAppdK from strain 2308, and of a double deletion mutant DELTApckADELTAppdK, also lacking phosphoenolpyruvate carboxykinase, both show a reduced growth phenotype, overview. But albeit impaired in growth, the DELTAppdK is still able to reach the replicative intracellular niche, but BABDELTAppdK fails to reach the chronic steady phase typical of virulent brucellae Brucella abortus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Brucella abortus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + pyruvate + phosphate Brucella abortus
-
 AMP + phosphoenolpyruvate + diphosphate
-
 r
ATP + pyruvate + phosphate Brucella abortus 2308
-
 AMP + phosphoenolpyruvate + diphosphate
-
 r

Organism

Organism UniProt Comment Textmining
Brucella abortus Q2YMM9
-
-
Brucella abortus 2308 Q2YMM9
-
-

Source Tissue

Source Tissue Comment Organism Textmining
macrophage
-
 Brucella abortus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + pyruvate + phosphate
-
 Brucella abortus AMP + phosphoenolpyruvate + diphosphate
-
 r
ATP + pyruvate + phosphate
-
 Brucella abortus 2308 AMP + phosphoenolpyruvate + diphosphate
-
 r

Subunits

Subunits Comment Organism
More putative Brucella abortus 2308 PpdK, encoded by BAB1_0525, represents a complete protein of 887 amino acids with the PEP-binding (amino acids 19 to 376) and the TIM barrel (amino acids 530 to 883) domains characteristic of PEP-utilizing enzymes Brucella abortus

Synonyms

Synonyms Comment Organism
BAB1_0525 locus name Brucella abortus
PPDK
-
 Brucella abortus
pyruvate phosphate dikinase
-
 Brucella abortus

Cofactor

Cofactor Comment Organism Structure
AMP
-
 Brucella abortus
ATP
-
 Brucella abortus

General Information

General Information Comment Organism
malfunction in gluconeogenic but not in rich media, growth of enzyme-deficient mutant DELTAppdK is severely impaired. In RAW 264.7 macrophages, the DELTAppdK mutant shows reduced multiplication, and studies with the DELTAppdK mutant confirm that it reaches the replicative niche. The mutant is attenuated in mice being cleared by week 10 Brucella abortus
metabolism the enzyme plays an important role in the central C metabolism between gluconeogenesis, glycolysis, and TCA cycle. Dysfunction of Brucella abortus fbp, glpX, ppdK, and mae but not of pckA or aceA homologues affects growth on gluconeogenic substrates in vitro Brucella abortus