Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diphosphate | competitive inhibitor | Zea mays |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.178 | - |
pyruvate | at pH 7.0 and 30°C | Zea mays | |
0.194 | - |
phosphoenolpyruvate | at pH 7.0 and 30°C | Zea mays |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | the enzyme requires Mg2+ for oligomerization | Zea mays | |
NH4+ | the enzyme requires NH4+ as a cofactor for optimal catalysis | Zea mays |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
95000 | - |
4 * 95000, the enzyme is maximally active as a homotetramer and is inactive in the dimeric and monomeric forms | Zea mays |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | the PPDK regulatory protein catalyses this light-dependent regulation of the enzyme by reversible phosphorylation of the active-site Thr456 residue | Zea mays |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | - |
Zea mays | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
AMP + phosphoenolpyruvate + diphosphate | - |
Zea mays | ATP + pyruvate + phosphate | - |
r | |
ATP + pyruvate + phosphate | - |
Zea mays | AMP + phosphoenolpyruvate + diphosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 95000, the enzyme is maximally active as a homotetramer and is inactive in the dimeric and monomeric forms | Zea mays |
Synonyms | Comment | Organism |
---|---|---|
PPDK | - |
Zea mays |
pyruvate, orthophosphate dikinase | - |
Zea mays |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
the pyruvate-forming reaction is strongly favoured at pH 7.0 | Zea mays |
8.3 | - |
the competency of the enzyme in catalyzing its phosphoenolpyruvate-forming reaction at pH 7.0 is dramatically reduced, having only 6% of the rate at pH 8.3 | Zea mays |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8.3 | the competency of the enzyme in catalyzing its phosphoenolpyruvate-forming reaction at pH 7.0 is dramatically reduced, having only 6% of the rate at pH 8.3 | Zea mays |
Organism | Comment | Expression |
---|---|---|
Zea mays | in the C4 pathway, enzyme activity is strictly regulated in an up/down manner by the level of incident light | up |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme plays a controlling role in the phosphoenolpyruvate-regeneration phase of the C4 photosynthetic pathway | Zea mays |