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Literature summary for 2.7.9.1 extracted from
- Nano-sized bacterial magnetic particles displaying pyruvate phosphate dikinase for pyrosequencing (2009), Biotechnol. Bioeng., 103, 130-137.
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | pyruvate phosphate dikinase, expressed onto bacterial magnetic particles, PPDK-BacMPs, is utilized in pyrosequencing | Microbispora rosea subsp. aerata |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into the vector pUMP16M13 | Microbispora rosea subsp. aerata |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 91000 | - |
- |
Microbispora rosea subsp. aerata |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| AMP + phosphoenolpyruvate + diphosphate | Microbispora rosea subsp. aerata | - |
ATP + pyruvate + phosphate | - |
r |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Microbispora rosea subsp. aerata | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| AMP + phosphoenolpyruvate + diphosphate | - |
Microbispora rosea subsp. aerata | ATP + pyruvate + phosphate | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PPDK | - |
Microbispora rosea subsp. aerata |
| pyruvate phosphate dikinase | - |
Microbispora rosea subsp. aerata |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
activity assay | Microbispora rosea subsp. aerata |
| 37 | - |
pyrosequencing by utilizing pyruvate phosphate dikinase, expressed onto bacterial magnetic particles, PPDK-BacMPs | Microbispora rosea subsp. aerata |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
45 | pyruvate phosphate dikinase, expressed onto bacterial magnetic particles, PPDK-BacMPs, shows stable enzymatic activity | Microbispora rosea subsp. aerata |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
activity assay | Microbispora rosea subsp. aerata |
| 7.75 | - |
pyrosequencing by utilizing pyruvate phosphate dikinase, expressed onto bacterial magnetic particles, PPDK-BacMPs | Microbispora rosea subsp. aerata |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9.5 | pyruvate phosphate dikinase, expressed onto bacterial magnetic particles, PPDK-BacMPs, shows stable enzymatic activity | Microbispora rosea subsp. aerata |
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Release 2023.1 (January 2023)
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