Application | Comment | Organism |
---|---|---|
agriculture | PPDK function during early cereal seed development, seed metabolism | Oryza sativa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | PPDK inactivation and protein degradation by posttranslational regulation during progressive seed development in rice, most abundant inactive forms of PPDK in endosperm of mature seeds | Oryza sativa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | - |
japonica variety Kitake of rice | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | during seed development posttranslational down-regulation of PPDK in activity and amount by regulatory threonyl-phosphorylation PPDK regulatory protein | Oryza sativa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | variety Kitake of rice, early cellularization stages of syncitial-endosperm during seed development, separated analysis of pericarp, embryo and aleurone and seed embryo, regulatory phosphorylation of PPDK in the non-green seed embryo and in green outer pericarp layer, no regulatory phosphorylation of PPDK in the endosperm and aleurone layer, pool of inactive PPDK in mature seed most abundant, immunoblot analysis, PPDK assay, inhibition assay | Oryza sativa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activity and amounts of PPDK downregulated during seed development in rice, tissue-specificy, immunoblot analysis of different seed fractions of rice, SDS-PAGE, spectrophotometric assay of PPDK acitivity, kinetics | Oryza sativa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyruvate + phosphate | - |
Oryza sativa | AMP + phosphoenolpyruvate + diphosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
More | phosphomonomer of about 95000 Dalton in rice as reference, SDS-PAGE, immunoblot | Oryza sativa |
Synonyms | Comment | Organism |
---|---|---|
PPDK | - |
Oryza sativa |
pyruvate, orthophosphate dikinase | - |
Oryza sativa |