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Literature summary for 2.7.9.1 extracted from

  • Chastain, C.J.; Heck, J.W.; Colquhoun, T.A.; Voge, D.G.; Gu, X.Y.
    Posttranslational regulation of pyruvate, orthophosphate dikinase in developing rice (Oryza sativa) seeds (2006), Planta, 224, 924-934.
    View publication on PubMed

Application

Application Comment Organism
agriculture PPDK function during early cereal seed development, seed metabolism Oryza sativa

Inhibitors

Inhibitors Comment Organism Structure
additional information PPDK inactivation and protein degradation by posttranslational regulation during progressive seed development in rice, most abundant inactive forms of PPDK in endosperm of mature seeds Oryza sativa

Organism

Organism UniProt Comment Textmining
Oryza sativa
-
japonica variety Kitake of rice
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Posttranslational Modification

Posttranslational Modification Comment Organism
additional information during seed development posttranslational down-regulation of PPDK in activity and amount by regulatory threonyl-phosphorylation PPDK regulatory protein Oryza sativa

Source Tissue

Source Tissue Comment Organism Textmining
seed variety Kitake of rice, early cellularization stages of syncitial-endosperm during seed development, separated analysis of pericarp, embryo and aleurone and seed embryo, regulatory phosphorylation of PPDK in the non-green seed embryo and in green outer pericarp layer, no regulatory phosphorylation of PPDK in the endosperm and aleurone layer, pool of inactive PPDK in mature seed most abundant, immunoblot analysis, PPDK assay, inhibition assay Oryza sativa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
activity and amounts of PPDK downregulated during seed development in rice, tissue-specificy, immunoblot analysis of different seed fractions of rice, SDS-PAGE, spectrophotometric assay of PPDK acitivity, kinetics Oryza sativa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + pyruvate + phosphate
-
Oryza sativa AMP + phosphoenolpyruvate + diphosphate
-
r

Subunits

Subunits Comment Organism
More phosphomonomer of about 95000 Dalton in rice as reference, SDS-PAGE, immunoblot Oryza sativa

Synonyms

Synonyms Comment Organism
PPDK
-
Oryza sativa
pyruvate, orthophosphate dikinase
-
Oryza sativa