Literature summary for 2.7.9.1 extracted from

Chastain, C.J.; Botschner, M.; Harrington, G.E.; Thompson, B.J.; Mills, S.E.; Sarath, G.; Chollet, R.
Further analysis of maize C(4) pyruvate,orthophosphate dikinase phosphorylation by its bifunctional regulatory protein using selective substitutions of the regulatory Thr-456 and catalytic His-458 residues (2000), Arch. Biochem. Biophys., 375, 165-170.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Zea mays

Protein Variants

Protein Variants	Comment	Organism
H458N	no activity	Zea mays
T456E	no activity	Zea mays
T456F	-	Zea mays
T456Y	-	Zea mays

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
95000	-	x * 95000 immunoblot analysis	Zea mays

Organism

Organism	UniProt	Comment	Textmining
Zea mays	-	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.043	-	T456F mutant	Zea mays
0.24	-	T456Y mutant	Zea mays
4.1	-	-	Zea mays

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + pyruvate + phosphate	-	Zea mays	AMP + phosphoenolpyruvate + diphosphate	-	r

Subunits

Subunits	Comment	Organism
?	x * 95000 immunoblot analysis	Zea mays

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Release 2023.1 (January 2023)