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Literature summary for 2.7.9.1 extracted from
- Expression and characterization of recombinant pyruvate phosphate dikinase from Entamoeba histolytica (1998), Biochim. Biophys. Acta, 1382, 47-54.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Entamoeba histolytica |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| tetrasodium 1-hydroxyethylidene biphosphonate | - |
Entamoeba histolytica |  |
| tetrasodium 1-hydroxymethylidene biphosphonate | - |
Entamoeba histolytica | |
| tetrasodium 1-hydroxynonane biphosphonate | - |
Entamoeba histolytica | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.005 | - |
AMP | less than 0.005 mM ,pH 6.3, 25ºC | Entamoeba histolytica | |
| 0.021 | - |
phosphoenolpyruvate | pH 6.3, 25ºC | Entamoeba histolytica | |
| 0.1 | - |
diphosphate | pH 6.3, 25ºC | Entamoeba histolytica | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | slight activation | Entamoeba histolytica | |
| Mg2+ | necessary for activity. Optimal concentration of 4.5 mM | Entamoeba histolytica | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 98000 | - |
x * 98000, SDS-PAGE | Entamoeba histolytica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Entamoeba histolytica | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| method that includes metal affinity chromatography | Entamoeba histolytica |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 12 | - |
recombinant enzyme | Entamoeba histolytica |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 22ºC, Tris-HCl buffer, pH 7.9, 5 mM imidazole, 50 mM NaCl, 2 weeks | Entamoeba histolytica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + pyruvate + phosphate | - |
Entamoeba histolytica | AMP + phosphoenolpyruvate + diphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 98000, SDS-PAGE | Entamoeba histolytica |
| tetramer | molecular filtration | Entamoeba histolytica |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | - |
inactive below 10ºC | Entamoeba histolytica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
ATP formation | Entamoeba histolytica |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.2 | - |
tetrasodium 1-hydroxyethylidene biphosphonate | pH 6.3, 25ºC | Entamoeba histolytica | |
| 4.3 | - |
tetrasodium 1-hydroxynonane biphosphonate | pH 6.3, 25ºC | Entamoeba histolytica | |
| 11 | - |
tetrasodium 1-hydroxymethylidene biphosphonate | pH 6.3, 25ºC | Entamoeba histolytica | |
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