Cloned (Comment) | Organism |
---|---|
25 kDa C-terminal and 35 kDa N-terminal deletion mutants expressed in Escherichia coli | [Clostridium] symbiosum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | 35 kDa C-terminal deletion mutant catalyzes the formation of a diphosphorylenzyme intermediate and diphosphate, but not the subsequent formation of phosphoenolpyruvate. A 25 kDa N-terminal deletion mutant catalyzes the second partial reaction but not the first one | [Clostridium] symbiosum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
[Clostridium] symbiosum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
two deletion mutants purified by a method that includes DEAE-cellulose and Sephadex G-200 chromatography | [Clostridium] symbiosum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyruvate + phosphate | - |
[Clostridium] symbiosum | AMP + phosphoenolpyruvate + diphosphate | - |
r |