Literature summary for 2.7.9.1 extracted from

Nakamoto, H.; Edwards, G.E.
Effect of adenine nucleotides on the reaction catalyzed by pyruvate,orthophosphate dikinase in maize (1987), Biochim. Biophys. Acta, 924, 360-368.

No PubMed abstract available

Search for more literature for 2.7.9.1

Inhibitors

Inhibitors	Comment	Organism	Structure
ADP	no inhibition up to 0.46 mM	Zea mays
AMP	competitive with respect to ATP	Zea mays
ATP	-	Zea mays
additional information	response of the enzyme to energy charge	Zea mays

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.023	-	ATP	pH 8, 22ºC	Zea mays

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
chloroplast	-	Zea mays	9507	-

Organism

Organism	UniProt	Comment	Textmining
Zea mays	-	maize	-

Purification (Commentary)

Purification (Comment)	Organism
method that includes successive chromatography through DE-52, hydroxyapatite, Sephadex G-200 and Blue agarose	Zea mays

Source Tissue

Source Tissue	Comment	Organism	Textmining
mesophyll	-	Zea mays	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + pyruvate + phosphate	-	Zea mays	AMP + phosphoenolpyruvate + diphosphate	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
22	-	assay at	Zea mays

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at, at pH 7.5 and pH 7.0 without ADP and AMP the enzyme activity is 61% and 38% of the activity at pH 8 respectively	Zea mays

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.036	-	ATP	pH 8, 22ºC	Zea mays

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Release 2023.1 (January 2023)