Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | - |
Acetobacter aceti | |
ATP | competitive to AMP | Acetobacter aceti | |
diphosphate | competitive to phosphate | Acetobacter aceti | |
phosphate | competitive to diphosphate | Acetobacter aceti | |
phosphoenolpyruvate | competitive to pyruvate | Acetobacter aceti | |
pyruvate | competitive to phosphoenolpyruvate | Acetobacter aceti |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
AMP | pH 7.1, 30ºC, pyruvate formation | Acetobacter aceti | |
0.027 | - |
pyruvate | pH 8, 30ºC, phosphoenolpyruvate formation | Acetobacter aceti | |
0.062 | - |
diphosphate | pH 7.1, 30ºC, pyruvate formation | Acetobacter aceti | |
0.13 | - |
phosphoenolpyruvate | pH 7.1, 30ºC, pyruvate formation | Acetobacter aceti | |
0.2 | - |
ATP | pH 8, 30ºC, phosphoenolpyruvate formation | Acetobacter aceti | |
0.83 | - |
phosphate | pH 8, 30ºC, phosphoenolpyruvate formation | Acetobacter aceti |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | cannot be replaced by other divalent ions | Acetobacter aceti |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
6 * 58000, SDS-PAGE | Acetobacter aceti |
320000 | - |
gel filtration on Sephadex G-200 | Acetobacter aceti |
330000 | - |
gel filtration on Sephrose 6B | Acetobacter aceti |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetobacter aceti | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using hydroxyapatite, Sephadex G-200 and DEAE column chromatography | Acetobacter aceti |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + pyruvate + phosphate = AMP + phosphoenolpyruvate + diphosphate | non-classical three-site tri ping-pong kinetics | Acetobacter aceti |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.9 | - |
- |
Acetobacter aceti |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyruvate + phosphate | - |
Acetobacter aceti | AMP + phosphoenolpyruvate + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 58000, SDS-PAGE | Acetobacter aceti |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Acetobacter aceti |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.1 | - |
pyruvate formation | Acetobacter aceti |
8 | - |
phosphoenolpyruvate formation | Acetobacter aceti |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.32 | - |
AMP | pH 8, 30ºC, phosphoenolpyruvate formation | Acetobacter aceti | |
0.36 | - |
diphosphate | pH 8, 30ºC, phosphoenolpyruvate formation | Acetobacter aceti | |
0.6 | - |
ATP | pH 7.1, 30ºC, pyruvate formation | Acetobacter aceti | |
0.76 | - |
pyruvate | pH 7.1, 30ºC, pyruvate formation | Acetobacter aceti | |
2 | - |
phosphoenolpyruvate | pH 8, 30ºC, phosphoenolpyruvate formation | Acetobacter aceti | |
6 | - |
phosphate | pH 7.1, 30ºC, pyruvate formation | Acetobacter aceti |