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Literature summary for 2.7.8.B13 extracted from
- Ubiquitous distribution of phosphatidylinositol phosphate synthase and archaetidylinositol phosphate synthase in bacteria and archaea, which contain inositol phospholipid (2013), Biochem. Biophys. Res. Commun., 443, 86-90.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | an artificial gene construct for a protein homologous to phosphatidylinositol phosphate synthase of Mycobacterium tuberculosis and archaetidylinositol phosphate synthase of Methanothermobacter thermautotrophicus is cloned in Escherichia coli and introduced into Corynebacterium glutamicum cells. The recombinant cells show afterwards both, phosphatidylinositol phosphate synthase and archaetidylinositol phosphate synthase activities, 175 pmol/h/mg and 174 pmol/h/mg, respectively, but no phosphatidylinositol synthase activity is detected, overview | Corynebacterium glutamicum |
| additional information | an artificial gene construct for a protein homologous to phosphatidylinositol phosphate synthase of Mycobacterium tuberculosis and archaetidylinositol phosphate synthase of Methanothermobacter thermautotrophicus is cloned in Escherichia coli and introduced into Mycobacterium bovis cells. The recombinant cells show afterwards both, phosphatidylinositol phosphate synthase and archaetidylinositol phosphate synthase activities, 138 pmol/h/mg and 5 pmol/h/mg, respectively, but no phosphatidylinositol synthase activity is detected, overview | Mycobacterium tuberculosis variant bovis |
| additional information | an artificial gene construct for a protein homologous to phosphatidylinositol phosphate synthase of Mycobacterium tuberculosis and archaetidylinositol phosphate synthase of Methanothermobacter thermautotrophicus is cloned in Escherichia coli and introduced into Propionibacterium acnes cells. The recombinant cells show afterwards both, phosphatidylinositol phosphate synthase and archaetidylinositol phosphate synthase activities, 88 pmol/h/mg and 14 pmol/h/mg, respectively, but no phosphatidylinositol synthase activity is detected, overview | Cutibacterium acnes |
| additional information | an artificial gene construct for a protein homologous to phosphatidylinositol phosphate synthase of Mycobacterium tuberculosis and archaetidylinositol phosphate synthase of Methanothermobacter thermautotrophicus is cloned in Escherichia coli and introduced into Rhodococcus equi cells. The recombinant cells show afterwards both, phosphatidylinositol phosphate synthase and archaetidylinositol phosphate synthase activities, 124 pmol/h/mg and 33 pmol/h/mg, respectively, but no phosphatidylinositol synthase activity is detected, overview | Rhodococcus equi |
| additional information | an artificial gene construct for a protein homologous to phosphatidylinositol phosphate synthase of Mycobacterium tuberculosis and archaetidylinositol phosphate synthase of Methanothermobacter thermautotrophicus is cloned in Escherichia coli and introduced into Streptomyces avermitilis cells. The recombinant cells show afterwards both, phosphatidylinositol phosphate synthase and archaetidylinositol phosphate synthase activities, 76 pmol/h/mg and 20 pmol/h/mg, respectively, but no phosphatidylinositol synthase activity is detected, overview | Streptomyces avermitilis |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Corynebacterium glutamicum |  |
| Mg2+ | required | Rhodococcus equi | |
| Mg2+ | required | Cutibacterium acnes | |
| Mg2+ | required | Streptomyces avermitilis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CDP-diacylglycerol + 1L-myo-inositol 1-phosphate | Corynebacterium glutamicum | specificity of PIP synthase for lipid substrates | CMP + 1-phosphatidyl-1D-myo-inositol 3-phosphate | - |
? | |
| CDP-diacylglycerol + 1L-myo-inositol 1-phosphate | Rhodococcus equi | specificity of PIP synthase for lipid substrates | CMP + 1-phosphatidyl-1D-myo-inositol 3-phosphate | - |
? | |
| CDP-diacylglycerol + 1L-myo-inositol 1-phosphate | Mycobacterium tuberculosis variant bovis | specificity of PIP synthase for lipid substrates | CMP + 1-phosphatidyl-1D-myo-inositol 3-phosphate | - |
? | |
| CDP-diacylglycerol + 1L-myo-inositol 1-phosphate | Cutibacterium acnes | specificity of PIP synthase for lipid substrates | CMP + 1-phosphatidyl-1D-myo-inositol 3-phosphate | - |
? | |
| CDP-diacylglycerol + 1L-myo-inositol 1-phosphate | Streptomyces avermitilis | specificity of PIP synthase for lipid substrates | CMP + 1-phosphatidyl-1D-myo-inositol 3-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Corynebacterium glutamicum | - |
- |
- |
| Cutibacterium acnes | - |
- |
- |
| Mycobacterium tuberculosis variant bovis | - |
- |
- |
| Rhodococcus equi | - |
- |
- |
| Streptomyces avermitilis | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CDP-diacylglycerol + 1L-myo-inositol 1-phosphate | - |
Corynebacterium glutamicum | CMP + 1-phosphatidyl-1D-myo-inositol 3-phosphate | - |
? | |
| CDP-diacylglycerol + 1L-myo-inositol 1-phosphate | - |
Rhodococcus equi | CMP + 1-phosphatidyl-1D-myo-inositol 3-phosphate | - |
? | |
| CDP-diacylglycerol + 1L-myo-inositol 1-phosphate | - |
Mycobacterium tuberculosis variant bovis | CMP + 1-phosphatidyl-1D-myo-inositol 3-phosphate | - |
? | |
| CDP-diacylglycerol + 1L-myo-inositol 1-phosphate | - |
Cutibacterium acnes | CMP + 1-phosphatidyl-1D-myo-inositol 3-phosphate | - |
? | |
| CDP-diacylglycerol + 1L-myo-inositol 1-phosphate | - |
Streptomyces avermitilis | CMP + 1-phosphatidyl-1D-myo-inositol 3-phosphate | - |
? | |
| CDP-diacylglycerol + 1L-myo-inositol 1-phosphate | specificity of PIP synthase for lipid substrates | Corynebacterium glutamicum | CMP + 1-phosphatidyl-1D-myo-inositol 3-phosphate | - |
? | |
| CDP-diacylglycerol + 1L-myo-inositol 1-phosphate | specificity of PIP synthase for lipid substrates | Rhodococcus equi | CMP + 1-phosphatidyl-1D-myo-inositol 3-phosphate | - |
? | |
| CDP-diacylglycerol + 1L-myo-inositol 1-phosphate | specificity of PIP synthase for lipid substrates | Mycobacterium tuberculosis variant bovis | CMP + 1-phosphatidyl-1D-myo-inositol 3-phosphate | - |
? | |
| CDP-diacylglycerol + 1L-myo-inositol 1-phosphate | specificity of PIP synthase for lipid substrates | Cutibacterium acnes | CMP + 1-phosphatidyl-1D-myo-inositol 3-phosphate | - |
? | |
| CDP-diacylglycerol + 1L-myo-inositol 1-phosphate | specificity of PIP synthase for lipid substrates | Streptomyces avermitilis | CMP + 1-phosphatidyl-1D-myo-inositol 3-phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| phosphatidylinositol phosphate synthase | - |
Corynebacterium glutamicum |
| phosphatidylinositol phosphate synthase | - |
Rhodococcus equi |
| phosphatidylinositol phosphate synthase | - |
Mycobacterium tuberculosis variant bovis |
| phosphatidylinositol phosphate synthase | - |
Cutibacterium acnes |
| phosphatidylinositol phosphate synthase | - |
Streptomyces avermitilis |
| PIP synthase | - |
Corynebacterium glutamicum |
| PIP synthase | - |
Rhodococcus equi |
| PIP synthase | - |
Mycobacterium tuberculosis variant bovis |
| PIP synthase | - |
Cutibacterium acnes |
| PIP synthase | - |
Streptomyces avermitilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Corynebacterium glutamicum |
| 37 | - |
assay at | Rhodococcus equi |
| 37 | - |
assay at | Cutibacterium acnes |
| 37 | - |
assay at | Streptomyces avermitilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Corynebacterium glutamicum |
| 8 | - |
assay at | Rhodococcus equi |
| 8 | - |
assay at | Cutibacterium acnes |
| 8 | - |
assay at | Streptomyces avermitilis |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | in bacteria, an inositol phospholipid biosynthetic pathway uses precursors inositol 1-phosphate and CDP-DAG to form phosphatidylinositol phosphate (PIP) as intermediate by action of the key enzyme PIP synthase. The intermediate is dephosphorylated to synthesize phosphatidylinositol. The biosynthetic pathway is universal in bacteria which contain inositol phospholipid | Corynebacterium glutamicum |
| metabolism | in bacteria, an inositol phospholipid biosynthetic pathway uses precursors inositol 1-phosphate and CDP-DAG to form phosphatidylinositol phosphate (PIP) as intermediate by action of the key enzyme PIP synthase. The intermediate is dephosphorylated to synthesize phosphatidylinositol. The biosynthetic pathway is universal in bacteria which contain inositol phospholipid | Rhodococcus equi |
| metabolism | in bacteria, an inositol phospholipid biosynthetic pathway uses precursors inositol 1-phosphate and CDP-DAG to form phosphatidylinositol phosphate (PIP) as intermediate by action of the key enzyme PIP synthase. The intermediate is dephosphorylated to synthesize phosphatidylinositol. The biosynthetic pathway is universal in bacteria which contain inositol phospholipid | Mycobacterium tuberculosis variant bovis |
| metabolism | in bacteria, an inositol phospholipid biosynthetic pathway uses precursors inositol 1-phosphate and CDP-DAG to form phosphatidylinositol phosphate (PIP) as intermediate by action of the key enzyme PIP synthase. The intermediate is dephosphorylated to synthesize phosphatidylinositol. The biosynthetic pathway is universal in bacteria which contain inositol phospholipid | Cutibacterium acnes |
| metabolism | in bacteria, an inositol phospholipid biosynthetic pathway uses precursors inositol 1-phosphate and CDP-DAG to form phosphatidylinositol phosphate (PIP) as intermediate by action of the key enzyme PIP synthase. The intermediate is dephosphorylated to synthesize phosphatidylinositol. The biosynthetic pathway is universal in bacteria which contain inositol phospholipid | Streptomyces avermitilis |
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