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Literature summary for 2.7.8.9 extracted from

  • Bretthauer, R.K.; Kozak, L.P.; Irwin, W.E.
    Phosphate and mannose transfer from guanosine diphosphate mannose to yeast mannan acceptors (1969), Biochem. Biophys. Res. Commun., 37, 820-827.
    View publication on PubMed
Search for more literature for 2.7.8.9

Inhibitors

Inhibitors Comment Organism Structure
GDP 0.5 mM: 70% inhibition Nakazawaea holstii
GMP 0.5 mM: 70% inhibition Nakazawaea holstii

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ 10 mM: optimum, divalent metal ion required Nakazawaea holstii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GDP-mannose + endogenous acceptor molecule Nakazawaea holstii
-
 GDP + endogenous acceptor molecule modified
-
 ?
GDP-mannose + endogenous acceptor molecule Nakazawaea holstii NRRL-Y 2448
-
 GDP + endogenous acceptor molecule modified
-
 ?

Organism

Organism UniProt Comment Textmining
Nakazawaea holstii
-
 NRRL-Y 2448
-
Nakazawaea holstii NRRL-Y 2448
-
 NRRL-Y 2448
-

Purification (Commentary)

Purification (Comment) Organism
partial Nakazawaea holstii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-mannose + endogenous acceptor molecule
-
 Nakazawaea holstii GDP + endogenous acceptor molecule modified
-
 ?
GDP-mannose + endogenous acceptor molecule crude extract, acceptor may be cell wall mannan, glycoprotein or exocellular phosphomannan Nakazawaea holstii GDP + endogenous acceptor molecule modified mannose 1-phosphate is transferred from GDP-mannose forming a 1,6'-phosphodiester linkage between 2 mannose residues (proposed) ?
GDP-mannose + endogenous acceptor molecule
-
 Nakazawaea holstii NRRL-Y 2448 GDP + endogenous acceptor molecule modified
-
 ?
GDP-mannose + endogenous acceptor molecule crude extract, acceptor may be cell wall mannan, glycoprotein or exocellular phosphomannan Nakazawaea holstii NRRL-Y 2448 GDP + endogenous acceptor molecule modified mannose 1-phosphate is transferred from GDP-mannose forming a 1,6'-phosphodiester linkage between 2 mannose residues (proposed) ?
additional information mannose 6-phosphate, GDP are ineffective Nakazawaea holstii ?
-
 ?
additional information mannose 6-phosphate, GDP are ineffective Nakazawaea holstii NRRL-Y 2448 ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Nakazawaea holstii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 assay at Nakazawaea holstii