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Literature summary for 2.7.8.8 extracted from
- Expression, purification, and characterization of phosphatidylserine synthase from Escherichia coli K12 in Bacillus subtilis (2009), J. Agric. Food Chem., 57, 122-126.
Application
| Application | Comment | Organism |
|---|---|---|
| food industry | phospholipids, especially phosphatidylserine, have many applications in functional food and pharmaceutical industries | Escherichia coli |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into the vector pBES for expression in Bacillus subtilis DB104 | Escherichia coli |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 52800 | - |
theoretical | Escherichia coli |
| 53000 | - |
determined by SDS-PAGE | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CDP-diacylglycerol + L-serine | Escherichia coli | - |
CMP + (3-sn-phosphatidyl)-L-serine | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P23830 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| after ammonium sulfate precipitation on a SP-Sepharose and a Sephadex G-75 gel column | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.34 | - |
extract supernatant | Escherichia coli |
| 2.62 | - |
after ammonium sulfate precipitation | Escherichia coli |
| 7.86 | - |
purification step SP-Sepharose HP | Escherichia coli |
| 13.62 | - |
purification step Sephadex G-75 | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CDP-diacylglycerol + L-serine | - |
Escherichia coli | CMP + (3-sn-phosphatidyl)-L-serine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CDP-diacylglycerol:L-serine O-phosphatidyltransferase | - |
Escherichia coli |
| phosphatidylserine synthase | - |
Escherichia coli |
| PSS | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
activity assay | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
transphosphatidylation | Escherichia coli |
| 8 | - |
hydrolysis | Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 9.5 | - |
Escherichia coli |
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Release 2023.1 (January 2023)
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