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Literature summary for 2.7.8.8 extracted from

  • Dowhan, W.; Larson, T.
    Phosphatidylserine synthase from Escherichia coli (1981), Methods Enzymol., 71, 561-571.
No PubMed abstract available
Search for more literature for 2.7.8.8

Activating Compound

Activating Compound Comment Organism Structure
additional information optimal activity is dependent on ionic strength, 0.3 or higher Escherichia coli
Triton X-100 enzyme is dependent on a nonionic detergent such as Triton X-100 Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
ribosome tightly bound to ribosome Escherichia coli 5840
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
 x * 54000, SDS-PAGE Escherichia coli
500000
-
 glycerol gradient centrifugation Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
CDP-diacylglycerol + L-serine = CMP + (3-sn-phosphatidyl)-L-serine the enzyme appears to catalyze a ping-pong reaction Escherichia coli
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
39
-
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CDP-1,2-dicaproyl-DL-glycerol + L-Ser
-
 Escherichia coli CMP + 3-O-sn-1,2-dicaproylphosphatidylserine
-
 ?
CDP-1,2-dipalmitoyl-L-glycerol + L-Ser
-
 Escherichia coli CMP + 1,2-dipalmitoylphosphatidylserine
-
 ?
CDP-diacylglycerol + H2O at 1% of the synthetic rate the enzyme catalyzes the hydrolysis of phosphatidylserine to CMP and phosphatidic acid Escherichia coli CMP + phosphatidic acid
-
 ?
CDP-diacylglycerol + L-Ser
-
 Escherichia coli CMP + 3-O-sn-phosphatidyl-L-serine
-
 ?
additional information the enzyme also catalyzes the exchange reaction between Ser and phosphatidylserine Escherichia coli ?
-
 ?
additional information enzyme catalyzes exchange reaction between CMP and CDP-diglyceride Escherichia coli ?
-
 ?

Subunits

Subunits Comment Organism
? x * 54000, SDS-PAGE Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8.5
-
 Escherichia coli