Literature summary for 2.7.8.7 extracted from

Dobb, K.S.; Kaye, S.J.; Beckmann, N.; Thain, J.L.; Stateva, L.; Birch, M.; Oliver, J.D.
Characterisation of the Candida albicans phosphopantetheinyl transferase Ppt2 as a potential antifungal drug target (2015), PLoS ONE, 10, e0143770.

Search for more literature for 2.7.8.7

Application

Application	Comment	Organism
drug development	the enzyme is a broad-spectrum target for design of antifungal drugs	Candida albicans

Cloned(Commentary)

Cloned (Comment)	Organism
gene PPT2, DNA and amino acid sequence determination and analysis, recombinant expression of tagged wild-type and mutant enzymes in Escherichia coli strain BL21 (DE3)	Candida albicans

Protein Variants

Protein Variants	Comment	Organism
additional information	cloning of PPT2 promoter replacement construct and a deletion construct from strain SN76, by placing one allele under the control of the regulatable MET3 promoter, and deleting the remaining allele. Phenotypes of the heterozygous and the conditional null mutant, overview	Candida albicans

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	dependent on	Candida albicans

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein]	Candida albicans	-	adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]	-	?

Organism

Organism	UniProt	Comment	Textmining
Candida albicans	Q5APF3	strain ATCC MYA-2876	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant tagged wild-type and mutant enzymes from Escherichia coli strain BL21 (DE3) by immobilized metal affinity chromatography	Candida albicans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein]	-	Candida albicans	adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]	-	?
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein]	catalytic activity of Ppt2 as a phosphopantetheinyl transferase and the acyl carrier protein Acp1 as a substrate, Acp12 is no substrate. The terminal thiol group of the 4'PPT is the site at which elongation occurs via thioester linkages and attachments are covalently linked	Candida albicans	adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]	-	?

Synonyms

Synonyms	Comment	Organism
C1_09480W	-	Candida albicans
phosphopantetheinyl transferase	-	Candida albicans
PPT2	-	Candida albicans
Ppt2p	-	Candida albicans
PPTase	-	Candida albicans

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
22	-	assay at room temperature	Candida albicans

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Candida albicans

General Information

General Information	Comment	Organism
physiological function	The 4'-phosphopantetheine (4'PPT) portion of coenzyme A (CoA) is an essential group for many carrier proteins and enzymes. Addition of this group is required for the correct function of polyketide synthase (PKS), non-ribosomal peptide synthetase and fatty acid synthase. The 4'PPT group is transferred to a highly conserved serine motif in the acceptor protein in a magnesium dependent reaction by phosphopantetheinyl transferases (PPTases). Gene PPT2 encoding a phosphopantetheinyl transferase and is essential for growth in Candida albicans	Candida albicans

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Release 2023.1 (January 2023)