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Literature summary for 2.7.8.7 extracted from
- A phosphopantetheinyl transferase that is essential for mitochondrial fatty acid biosynthesis (2015), Plant J., 84, 718-732.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene AT2G02770, a pseudogene, DNA and amino acid sequence determination and analysis, gene modeling | Arabidopsis thaliana |
| gene AT3G11470, encoding the mitchondrial isozyme, has three splicing variants AT3G11470.1, AT3G11470.2, and AT3G11470.3, which are the result of alternative slicing that occurs in removing the second intron, DNA and amino acid sequence determination and analysis. The AT3G11470.3 protein is inactive. Quantitative RT-PCR expression analysis | Arabidopsis thaliana |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | a T-DNA-tagged mutant allele (SALK_152625C) of AT2G02770 was identified in which the insertion resides in the COP1 interacting protein domain | Arabidopsis thaliana |
| additional information | generation of mtPPT enzyme mutant plants, phenotype, overview | Arabidopsis thaliana |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | the mitochondrial localization of the AT3G11470-encoded proteins is validated by the ability of their N-terminal 80-residue leader sequence to guide a chimeric GFP protein to this organelle | Arabidopsis thaliana | 5739 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein] | Arabidopsis thaliana | the mitochondrial enzyme catalyzes the phosphopantetheinylation and thus activation of mitochondrial acyl carrier protein (mtACP) of mitochondrial fatty acid synthase (mtFAS) | adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | F4IRA7 | - |
- |
| Arabidopsis thaliana | Q8VYK1 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| flower | highest expression of mtPPT | Arabidopsis thaliana | - |
| root | - |
Arabidopsis thaliana | - |
| rosette leaf | - |
Arabidopsis thaliana | - |
| silique | - |
Arabidopsis thaliana | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein] | - |
Arabidopsis thaliana | adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
? | |
| CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein] | the mitochondrial enzyme catalyzes the phosphopantetheinylation and thus activation of mitochondrial acyl carrier protein (mtACP) of mitochondrial fatty acid synthase (mtFAS) | Arabidopsis thaliana | adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
? | |
| additional information | analysis of enzyme activities with three recombinant apo-mtACP isoforms, i.e. mtACP1 (AT2G44620), mtACP2 (AT1G65290) and mtACP3 (AT5G47630), overview | Arabidopsis thaliana | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | the protein structure model fuses an N-terminal COP1 interacting protein domain with a C-terminal Sfp-like PPT domain. The PPT domain of the predicted AT2G02770 protein shares 73% similarity and 67% identity with the mtPPT protein encoded by splicing variant AT3G11470.1 | Arabidopsis thaliana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AT2G02770 | - |
Arabidopsis thaliana |
| mitochondrial phosphopantetheinyl transferase | - |
Arabidopsis thaliana |
| mtPPT | - |
Arabidopsis thaliana |
| phosphopantetheinyl transferase | - |
Arabidopsis thaliana |
| PPTase | - |
Arabidopsis thaliana |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | phylogenetic analyses of plant PPT/PPT-like homologues, overview | Arabidopsis thaliana |
| malfunction | a T-DNA-tagged null mutant mtppt-1 allele shows an embryo-lethal phenotype. Arabidopsis thaliana RNAi transgenic lines with reduced mtPPT expression display typical phenotypes associated with a deficiency in the mtFAS system, namely miniaturized plant morphology, slow growth, reduced lipoylation of mitochondrial proteins, and the hyperaccumulation of photorespiratory intermediates, glycine and glycolate. the seeds in the mtppt-1 mutant plants failed to develop normally. These morphological and metabolic alterations are reversed when these plants are grown in a non-photorespiratory condition (i.e. 1% CO2 atmosphere), because they are a consequence of a deficiency in photorespiration due to the reduced lipoylation of the photorespiratory glycine decarboxylase. Phenotypes, detailed overview | Arabidopsis thaliana |
| physiological function | the mitochondrial enzyme catalyzes the phosphopantetheinylation and thus activation of mitochondrial acyl carrier protein (mtACP) of mitochondrial fatty acid synthase (mtFAS). A crucial role of mtPPT for embryogenesis. MtPPT contributes to mtFAS and photorespiration | Arabidopsis thaliana |
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