Cloned (Comment) | Organism |
---|---|
gene BSU03570, recombinant expression of His-tagged enzyme from Bacillus subtilis in Escherichia coli | Bacillus subtilis |
gene LMJF_20_0830, recombinant expression of His-tagged enzyme from Leishmania major in Escherichia coli | Leishmania major |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | complete inhibition at 50 mM | Bacillus subtilis | |
EDTA | complete inhibition at 50 mM | Leishmania major |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | quantitative kinetic measurements, steady-state kinetics | Bacillus subtilis | |
0.0038 | - |
apo-[acyl-carrier protein] | pH 8.0, 37°C, recombinant His-tagged enzyme, mutant F44M ACP substrate from Leishmania major | Bacillus subtilis | |
0.0048 | - |
apo-[acyl-carrier protein] | pH 8.0, 37°C, recombinant His-tagged enzyme, mutant N35D/Q48E ACP substrate from Leishmania major | Bacillus subtilis | |
0.00524 | - |
apo-[acyl-carrier protein] | pH 8.0, 37°C, recombinant His-tagged enzyme, mutant N35D/F44M ACP substrate from Leishmania major | Bacillus subtilis | |
0.0055 | - |
apo-[acyl-carrier protein] | pH 8.0, 37°C, recombinant His-tagged enzyme, mutant N35D/F44M/Q48E ACP substrate from Leishmania major | Bacillus subtilis | |
0.0063 | - |
apo-[acyl-carrier protein] | pH 8.0, 37°C, recombinant His-tagged enzyme, wild-type ACP substrate from Leishmania major | Bacillus subtilis | |
0.0269 | - |
apo-[acyl-carrier protein] | pH 8.0, 37°C, recombinant His-tagged enzyme, mutant N35D ACP substrate from Leishmania major | Bacillus subtilis | |
0.101 | - |
apo-[acyl-carrier protein] | pH 8.0, 37°C, recombinant His-tagged enzyme, ACP substrate from Mycobacterium tuberculosis | Bacillus subtilis | |
0.12 | - |
apo-[acyl-carrier protein] | pH 8.0, 37°C, recombinant His-tagged enzyme, Escherichia coli ACP substrate | Bacillus subtilis | |
0.13 | - |
apo-[acyl-carrier protein] | pH 8.0, 37°C, recombinant His-tagged enzyme, Escherichia coli mutant M44F ACP substrate | Bacillus subtilis | |
0.215 | - |
apo-[acyl-carrier protein] | pH 8.0, 37°C, recombinant His-tagged enzyme, ACP substrate from Plasmodium falciparum | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Bacillus subtilis | |
Mg2+ | required | Leishmania major |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein] | Bacillus subtilis | - |
adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
? | |
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein] | Leishmania major | - |
adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
? | |
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein] | Bacillus subtilis 168 | - |
adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | P39135 | - |
- |
Bacillus subtilis 168 | P39135 | - |
- |
Leishmania major | Q4QCW3 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Bacillus subtilis from Escherichia coli | Bacillus subtilis |
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography | Leishmania major |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein] | - |
Bacillus subtilis | adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
? | |
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein] | - |
Leishmania major | adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
? | |
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein] | assay with ACP substrates from Leishmania major (LmACP), Escherichia coli, Plasmodium falciparum, Mycobacterium tuberculosis, and Homo sapiens. The structure of the holo-acyl carrier protein of Leishmania major is similar to other type II ACPs, comprising a four-helix bundle, enclosing a hydrophobic core, but it displays a remarkably different phosphopantetheinyl transferase binding interface. Two- and three-dimensional NMR structure analysis | Leishmania major | adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
? | |
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein] | - |
Bacillus subtilis 168 | adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
? | |
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein] | assay with wild-type and mutant ACP substrates from Leishmania major (LmACP), mutants N35D, F44M, and F44A, the double mutants N35D/F44M and N35D/Q48E, triple mutant N35D/F44M/Q48E of LmACP, and with Escherichia coli ACP, the M44F mutant of Escherichia coli ACP, Plasmmodium falciparum ACP, and Mycobacterium tuberculosis ACP. No activity with LmACP F44A mutant | Bacillus subtilis | ? | - |
? | |
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein] | assay with wild-type and mutant ACP substrates from Leishmania major (LmACP), mutants N35D, F44M, and F44A, the double mutants N35D/F44M and N35D/Q48E, triple mutant N35D/F44M/Q48E of LmACP, and with Escherichia coli ACP, the M44F mutant of Escherichia coli ACP, Plasmmodium falciparum ACP, and Mycobacterium tuberculosis ACP. No activity with LmACP F44A mutant | Bacillus subtilis 168 | ? | - |
? | |
additional information | recombinant expression of ACP substrates from Leishmania major (LmACP), Escherichia coli, Plasmodium falciparum, Mycobacterium tuberculosis, and Homo sapiens in Escherichia coli and purification by ion exchange chromatography. LmACP does not interact with the bacterial group I, 4'-phosphopantetheinyl transferase. Residues Asn 35 and Phe 44, present in LmACP modulate its interaction with AcpS | Leishmania major | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
4'-phosphopantetheinyl transferase | - |
Bacillus subtilis |
4'-phosphopantetheinyl transferase | - |
Leishmania major |
BcPPT | - |
Bacillus subtilis |
BSU03570 | - |
Bacillus subtilis |
LMJF_20_0830 | - |
Leishmania major |
LmPPT | - |
Leishmania major |
phosphopantetheinyl transferase | - |
Bacillus subtilis |
phosphopantetheinyl transferase | - |
Leishmania major |
Sfp | - |
Bacillus subtilis |
sfp lpa-8 | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bacillus subtilis |
37 | - |
assay at | Leishmania major |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00017 | - |
apo-[acyl-carrier protein] | pH 8.0, 37°C, recombinant His-tagged enzyme, mutant F44M ACP substrate from Leishmania major | Bacillus subtilis | |
0.00083 | - |
apo-[acyl-carrier protein] | pH 8.0, 37°C, recombinant His-tagged enzyme, wild-type ACP substrate from Leishmania major | Bacillus subtilis | |
0.001 | - |
apo-[acyl-carrier protein] | pH 8.0, 37°C, recombinant His-tagged enzyme, mutant N35D/F44M/Q48E ACP substrate from Leishmania major | Bacillus subtilis | |
0.0012 | - |
apo-[acyl-carrier protein] | pH 8.0, 37°C, recombinant His-tagged enzyme, mutant N35D/F44M ACP substrate from Leishmania major | Bacillus subtilis | |
0.0017 | - |
apo-[acyl-carrier protein] | pH 8.0, 37°C, recombinant His-tagged enzyme, mutant N35D/Q48E ACP substrate from Leishmania major | Bacillus subtilis | |
0.0038 | - |
apo-[acyl-carrier protein] | pH 8.0, 37°C, recombinant His-tagged enzyme, mutant N35D ACP substrate from Leishmania major | Bacillus subtilis | |
0.0082 | - |
apo-[acyl-carrier protein] | pH 8.0, 37°C, recombinant His-tagged enzyme, Escherichia coli mutant M44F ACP substrate | Bacillus subtilis | |
0.0103 | - |
apo-[acyl-carrier protein] | pH 8.0, 37°C, recombinant His-tagged enzyme, ACP substrate from Mycobacterium tuberculosis | Bacillus subtilis | |
0.014 | - |
apo-[acyl-carrier protein] | pH 8.0, 37°C, recombinant His-tagged enzyme, Escherichia coli ACP substrate | Bacillus subtilis | |
0.0192 | - |
apo-[acyl-carrier protein] | pH 8.0, 37°C, recombinant His-tagged enzyme, ACP substrate from Plasmodium falciparum | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Bacillus subtilis |
8 | - |
assay at | Leishmania major |