BRENDA - Enzyme Database show
show all sequences of 2.7.8.7

The structure of the holo-acyl carrier protein of Leishmania major displays a remarkably different phosphopantetheinyl transferase binding interface

Kumar, A.; Arya, R.; Makwana, P.K.; Dangi, R.S.; Yadav, U.; Surolia, A.; Kundu, S.; Sundd, M.; Biochemistry 54, 5632-5645 (2015)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene BSU03570, recombinant expression of His-tagged enzyme from Bacillus subtilis in Escherichia coli
Bacillus subtilis
gene LMJF_20_0830, recombinant expression of His-tagged enzyme from Leishmania major in Escherichia coli
Leishmania major
Inhibitors
Inhibitors
Commentary
Organism
Structure
EDTA
complete inhibition at 50 mM
Bacillus subtilis
EDTA
complete inhibition at 50 mM
Leishmania major
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
quantitative kinetic measurements, steady-state kinetics
Bacillus subtilis
0.0038
-
apo-[acyl-carrier protein]
pH 8.0, 37°C, recombinant His-tagged enzyme, mutant F44M ACP substrate from Leishmania major
Bacillus subtilis
0.0048
-
apo-[acyl-carrier protein]
pH 8.0, 37°C, recombinant His-tagged enzyme, mutant N35D/Q48E ACP substrate from Leishmania major
Bacillus subtilis
0.00524
-
apo-[acyl-carrier protein]
pH 8.0, 37°C, recombinant His-tagged enzyme, mutant N35D/F44M ACP substrate from Leishmania major
Bacillus subtilis
0.0055
-
apo-[acyl-carrier protein]
pH 8.0, 37°C, recombinant His-tagged enzyme, mutant N35D/F44M/Q48E ACP substrate from Leishmania major
Bacillus subtilis
0.0063
-
apo-[acyl-carrier protein]
pH 8.0, 37°C, recombinant His-tagged enzyme, wild-type ACP substrate from Leishmania major
Bacillus subtilis
0.0269
-
apo-[acyl-carrier protein]
pH 8.0, 37°C, recombinant His-tagged enzyme, mutant N35D ACP substrate from Leishmania major
Bacillus subtilis
0.101
-
apo-[acyl-carrier protein]
pH 8.0, 37°C, recombinant His-tagged enzyme, ACP substrate from Mycobacterium tuberculosis
Bacillus subtilis
0.12
-
apo-[acyl-carrier protein]
pH 8.0, 37°C, recombinant His-tagged enzyme, Escherichia coli ACP substrate
Bacillus subtilis
0.13
-
apo-[acyl-carrier protein]
pH 8.0, 37°C, recombinant His-tagged enzyme, Escherichia coli mutant M44F ACP substrate
Bacillus subtilis
0.215
-
apo-[acyl-carrier protein]
pH 8.0, 37°C, recombinant His-tagged enzyme, ACP substrate from Plasmodium falciparum
Bacillus subtilis
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Bacillus subtilis
Mg2+
required
Leishmania major
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein]
Bacillus subtilis
-
adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
-
?
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein]
Leishmania major
-
adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
-
?
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein]
Bacillus subtilis 168
-
adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus subtilis
P39135
-
-
Bacillus subtilis 168
P39135
-
-
Leishmania major
Q4QCW3
-
-
Purification (Commentary)
Commentary
Organism
recombinant His-tagged enzyme from Bacillus subtilis from Escherichia coli
Bacillus subtilis
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography
Leishmania major
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein]
-
737715
Bacillus subtilis
adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
-
-
?
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein]
-
737715
Leishmania major
adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
-
-
?
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein]
assay with ACP substrates from Leishmania major (LmACP), Escherichia coli, Plasmodium falciparum, Mycobacterium tuberculosis, and Homo sapiens. The structure of the holo-acyl carrier protein of Leishmania major is similar to other type II ACPs, comprising a four-helix bundle, enclosing a hydrophobic core, but it displays a remarkably different phosphopantetheinyl transferase binding interface. Two- and three-dimensional NMR structure analysis
737715
Leishmania major
adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
-
-
?
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein]
-
737715
Bacillus subtilis 168
adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
-
-
?
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein]
assay with wild-type and mutant ACP substrates from Leishmania major (LmACP), mutants N35D, F44M, and F44A, the double mutants N35D/F44M and N35D/Q48E, triple mutant N35D/F44M/Q48E of LmACP, and with Escherichia coli ACP, the M44F mutant of Escherichia coli ACP, Plasmmodium falciparum ACP, and Mycobacterium tuberculosis ACP. No activity with LmACP F44A mutant
737715
Bacillus subtilis
?
-
-
-
?
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein]
assay with wild-type and mutant ACP substrates from Leishmania major (LmACP), mutants N35D, F44M, and F44A, the double mutants N35D/F44M and N35D/Q48E, triple mutant N35D/F44M/Q48E of LmACP, and with Escherichia coli ACP, the M44F mutant of Escherichia coli ACP, Plasmmodium falciparum ACP, and Mycobacterium tuberculosis ACP. No activity with LmACP F44A mutant
737715
Bacillus subtilis 168
?
-
-
-
?
additional information
recombinant expression of ACP substrates from Leishmania major (LmACP), Escherichia coli, Plasmodium falciparum, Mycobacterium tuberculosis, and Homo sapiens in Escherichia coli and purification by ion exchange chromatography. LmACP does not interact with the bacterial group I, 4'-phosphopantetheinyl transferase. Residues Asn 35 and Phe 44, present in LmACP modulate its interaction with AcpS
737715
Leishmania major
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Bacillus subtilis
37
-
assay at
Leishmania major
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.00017
-
apo-[acyl-carrier protein]
pH 8.0, 37°C, recombinant His-tagged enzyme, mutant F44M ACP substrate from Leishmania major
Bacillus subtilis
0.00083
-
apo-[acyl-carrier protein]
pH 8.0, 37°C, recombinant His-tagged enzyme, wild-type ACP substrate from Leishmania major
Bacillus subtilis
0.001
-
apo-[acyl-carrier protein]
pH 8.0, 37°C, recombinant His-tagged enzyme, mutant N35D/F44M/Q48E ACP substrate from Leishmania major
Bacillus subtilis
0.0012
-
apo-[acyl-carrier protein]
pH 8.0, 37°C, recombinant His-tagged enzyme, mutant N35D/F44M ACP substrate from Leishmania major
Bacillus subtilis
0.0017
-
apo-[acyl-carrier protein]
pH 8.0, 37°C, recombinant His-tagged enzyme, mutant N35D/Q48E ACP substrate from Leishmania major
Bacillus subtilis
0.0038
-
apo-[acyl-carrier protein]
pH 8.0, 37°C, recombinant His-tagged enzyme, mutant N35D ACP substrate from Leishmania major
Bacillus subtilis
0.0082
-
apo-[acyl-carrier protein]
pH 8.0, 37°C, recombinant His-tagged enzyme, Escherichia coli mutant M44F ACP substrate
Bacillus subtilis
0.0103
-
apo-[acyl-carrier protein]
pH 8.0, 37°C, recombinant His-tagged enzyme, ACP substrate from Mycobacterium tuberculosis
Bacillus subtilis
0.014
-
apo-[acyl-carrier protein]
pH 8.0, 37°C, recombinant His-tagged enzyme, Escherichia coli ACP substrate
Bacillus subtilis
0.0192
-
apo-[acyl-carrier protein]
pH 8.0, 37°C, recombinant His-tagged enzyme, ACP substrate from Plasmodium falciparum
Bacillus subtilis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Bacillus subtilis
8
-
assay at
Leishmania major
Cloned(Commentary) (protein specific)
Commentary
Organism
gene BSU03570, recombinant expression of His-tagged enzyme from Bacillus subtilis in Escherichia coli
Bacillus subtilis
gene LMJF_20_0830, recombinant expression of His-tagged enzyme from Leishmania major in Escherichia coli
Leishmania major
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
EDTA
complete inhibition at 50 mM
Bacillus subtilis
EDTA
complete inhibition at 50 mM
Leishmania major
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
quantitative kinetic measurements, steady-state kinetics
Bacillus subtilis
0.0038
-
apo-[acyl-carrier protein]
pH 8.0, 37°C, recombinant His-tagged enzyme, mutant F44M ACP substrate from Leishmania major
Bacillus subtilis
0.0048
-
apo-[acyl-carrier protein]
pH 8.0, 37°C, recombinant His-tagged enzyme, mutant N35D/Q48E ACP substrate from Leishmania major
Bacillus subtilis
0.00524
-
apo-[acyl-carrier protein]
pH 8.0, 37°C, recombinant His-tagged enzyme, mutant N35D/F44M ACP substrate from Leishmania major
Bacillus subtilis
0.0055
-
apo-[acyl-carrier protein]
pH 8.0, 37°C, recombinant His-tagged enzyme, mutant N35D/F44M/Q48E ACP substrate from Leishmania major
Bacillus subtilis
0.0063
-
apo-[acyl-carrier protein]
pH 8.0, 37°C, recombinant His-tagged enzyme, wild-type ACP substrate from Leishmania major
Bacillus subtilis
0.0269
-
apo-[acyl-carrier protein]
pH 8.0, 37°C, recombinant His-tagged enzyme, mutant N35D ACP substrate from Leishmania major
Bacillus subtilis
0.101
-
apo-[acyl-carrier protein]
pH 8.0, 37°C, recombinant His-tagged enzyme, ACP substrate from Mycobacterium tuberculosis
Bacillus subtilis
0.12
-
apo-[acyl-carrier protein]
pH 8.0, 37°C, recombinant His-tagged enzyme, Escherichia coli ACP substrate
Bacillus subtilis
0.13
-
apo-[acyl-carrier protein]
pH 8.0, 37°C, recombinant His-tagged enzyme, Escherichia coli mutant M44F ACP substrate
Bacillus subtilis
0.215
-
apo-[acyl-carrier protein]
pH 8.0, 37°C, recombinant His-tagged enzyme, ACP substrate from Plasmodium falciparum
Bacillus subtilis
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Bacillus subtilis
Mg2+
required
Leishmania major
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein]
Bacillus subtilis
-
adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
-
?
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein]
Leishmania major
-
adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
-
?
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein]
Bacillus subtilis 168
-
adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His-tagged enzyme from Bacillus subtilis from Escherichia coli
Bacillus subtilis
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography
Leishmania major
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein]
-
737715
Bacillus subtilis
adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
-
-
?
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein]
-
737715
Leishmania major
adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
-
-
?
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein]
assay with ACP substrates from Leishmania major (LmACP), Escherichia coli, Plasmodium falciparum, Mycobacterium tuberculosis, and Homo sapiens. The structure of the holo-acyl carrier protein of Leishmania major is similar to other type II ACPs, comprising a four-helix bundle, enclosing a hydrophobic core, but it displays a remarkably different phosphopantetheinyl transferase binding interface. Two- and three-dimensional NMR structure analysis
737715
Leishmania major
adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
-
-
?
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein]
-
737715
Bacillus subtilis 168
adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
-
-
?
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein]
assay with wild-type and mutant ACP substrates from Leishmania major (LmACP), mutants N35D, F44M, and F44A, the double mutants N35D/F44M and N35D/Q48E, triple mutant N35D/F44M/Q48E of LmACP, and with Escherichia coli ACP, the M44F mutant of Escherichia coli ACP, Plasmmodium falciparum ACP, and Mycobacterium tuberculosis ACP. No activity with LmACP F44A mutant
737715
Bacillus subtilis
?
-
-
-
?
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein]
assay with wild-type and mutant ACP substrates from Leishmania major (LmACP), mutants N35D, F44M, and F44A, the double mutants N35D/F44M and N35D/Q48E, triple mutant N35D/F44M/Q48E of LmACP, and with Escherichia coli ACP, the M44F mutant of Escherichia coli ACP, Plasmmodium falciparum ACP, and Mycobacterium tuberculosis ACP. No activity with LmACP F44A mutant
737715
Bacillus subtilis 168
?
-
-
-
?
additional information
recombinant expression of ACP substrates from Leishmania major (LmACP), Escherichia coli, Plasmodium falciparum, Mycobacterium tuberculosis, and Homo sapiens in Escherichia coli and purification by ion exchange chromatography. LmACP does not interact with the bacterial group I, 4'-phosphopantetheinyl transferase. Residues Asn 35 and Phe 44, present in LmACP modulate its interaction with AcpS
737715
Leishmania major
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Bacillus subtilis
37
-
assay at
Leishmania major
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.00017
-
apo-[acyl-carrier protein]
pH 8.0, 37°C, recombinant His-tagged enzyme, mutant F44M ACP substrate from Leishmania major
Bacillus subtilis
0.00083
-
apo-[acyl-carrier protein]
pH 8.0, 37°C, recombinant His-tagged enzyme, wild-type ACP substrate from Leishmania major
Bacillus subtilis
0.001
-
apo-[acyl-carrier protein]
pH 8.0, 37°C, recombinant His-tagged enzyme, mutant N35D/F44M/Q48E ACP substrate from Leishmania major
Bacillus subtilis
0.0012
-
apo-[acyl-carrier protein]
pH 8.0, 37°C, recombinant His-tagged enzyme, mutant N35D/F44M ACP substrate from Leishmania major
Bacillus subtilis
0.0017
-
apo-[acyl-carrier protein]
pH 8.0, 37°C, recombinant His-tagged enzyme, mutant N35D/Q48E ACP substrate from Leishmania major
Bacillus subtilis
0.0038
-
apo-[acyl-carrier protein]
pH 8.0, 37°C, recombinant His-tagged enzyme, mutant N35D ACP substrate from Leishmania major
Bacillus subtilis
0.0082
-
apo-[acyl-carrier protein]
pH 8.0, 37°C, recombinant His-tagged enzyme, Escherichia coli mutant M44F ACP substrate
Bacillus subtilis
0.0103
-
apo-[acyl-carrier protein]
pH 8.0, 37°C, recombinant His-tagged enzyme, ACP substrate from Mycobacterium tuberculosis
Bacillus subtilis
0.014
-
apo-[acyl-carrier protein]
pH 8.0, 37°C, recombinant His-tagged enzyme, Escherichia coli ACP substrate
Bacillus subtilis
0.0192
-
apo-[acyl-carrier protein]
pH 8.0, 37°C, recombinant His-tagged enzyme, ACP substrate from Plasmodium falciparum
Bacillus subtilis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Bacillus subtilis
8
-
assay at
Leishmania major
Other publictions for EC 2.7.8.7
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737568
Wang
The substrate promiscuity of a ...
Streptomyces chattanoogensis, Streptomyces chattanoogensis L10
Arch. Microbiol.
198
193-197
2016
-
1
1
-
-
-
-
-
-
1
-
2
-
4
-
-
1
-
-
-
-
-
6
-
1
-
-
-
1
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
1
-
-
-
-
6
-
1
-
-
-
1
-
-
-
-
2
2
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-
739683
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Characterization of discrete p ...
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6
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-
-
1
-
-
-
-
-
-
-
-
10
-
12
-
-
1
-
-
-
-
-
20
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
10
-
-
-
5
-
-
-
-
20
-
-
-
-
-
-
-
-
-
-
3
12
-
-
-
737715
Kumar
The structure of the holo-acyl ...
Bacillus subtilis 168, Bacillus subtilis, Leishmania major
Biochemistry
54
5632-5645
2015
-
-
2
-
-
-
2
11
-
2
-
3
-
5
-
-
2
-
-
-
-
-
7
-
2
-
-
10
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
2
-
11
-
2
-
3
-
-
-
2
-
-
-
-
7
-
2
-
-
10
2
-
-
-
-
-
-
-
-
-
738868
Kim
Characterization of NpgA, a 4' ...
Aspergillus nidulans, Aspergillus nidulans SNT611
J. Microbiol.
53
21-31
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-
-
1
-
1
-
-
-
-
1
1
2
-
5
-
-
1
-
-
5
-
-
4
2
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
1
2
-
-
-
1
-
5
-
-
4
2
1
-
-
-
1
-
-
-
-
3
3
-
-
-
739319
Guan
A phosphopantetheinyl transfer ...
Arabidopsis thaliana
Plant J.
84
718-732
2015
-
-
1
-
1
-
-
-
2
-
-
1
-
4
-
-
-
-
-
4
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
2
-
-
-
-
-
2
-
-
1
-
-
-
-
-
4
-
-
3
1
-
-
-
-
-
-
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-
-
3
3
-
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-
739435
Dobb
Characterisation of the Candid ...
Candida albicans
PLoS ONE
10
e0143770
2015
-
1
1
-
1
-
-
-
-
1
-
1
-
3
-
-
1
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
1
1
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
737325
Vickery
Structure, biochemistry, and i ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv, Mycobacterium ulcerans
ACS Chem. Biol.
9
1939-1944
2014
-
-
2
2
6
-
11
-
-
2
-
3
-
6
-
-
2
-
-
-
-
-
3
2
-
-
-
-
-
-
-
-
-
-
19
-
-
2
-
2
6
-
19
11
-
-
-
2
-
3
-
-
-
2
-
-
-
-
3
2
-
-
-
-
-
-
-
-
-
4
4
-
-
-
738470
Kosa
Fluorescent techniques for dis ...
Homo sapiens
J. Antibiot.
67
113-120
2014
-
-
1
-
-
-
-
-
-
1
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1
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3
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2
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1
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1
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1
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1
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1
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-
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2
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
738932
Jung
Crystal structure of the essen ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis ATCC 25618
J. Struct. Biol.
188
274-278
2014
-
1
1
1
-
-
-
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1
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2
-
5
-
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1
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-
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4
1
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1
1
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1
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1
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2
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1
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
739157
Beld
The phosphopantetheinyl transf ...
Bacillus anthracis, Bacillus licheniformis, Bacillus subtilis 168, Bacillus subtilis, Brevibacillus brevis, Burkholderia cenocepacia, Burkholderia pseudomallei, Burkholderia pseudomallei K96243, Escherichia coli, Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv, Pseudomonas aeruginosa, Pseudomonas aeruginosa DSM 22644, Serratia marcescens subsp. marcescens, Serratia marcescens subsp. marcescens Db11, Streptomyces coelicolor, Streptomyces coelicolor ATCC BAA-471, Vibrio anguillarum, Vibrio anguillarum ATCC 68554, Vibrio cholerae, Xanthomonas albilineans, Xanthomonas albilineans GPE PC73, Yersinia pestis
Nat. Prod. Rep.
31
61-108
2014
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1
8
-
1
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-
-
-
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2
28
-
30
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52
5
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1
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-
-
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1
10
-
-
1
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-
4
28
-
-
-
-
-
-
-
-
52
7
-
-
-
-
-
1
-
-
1
31
42
1
-
-
739377
Contreras-Cornejo
-
The 4-phosphopantetheinyl tran ...
Trichoderma virens
Plant Soil
379
261-274
2014
-
-
-
-
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2
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2
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4
-
-
-
-
-
-
-
-
-
-
-
-
-
2
4
-
-
-
721361
Jiang
Improvement of natamycin produ ...
Streptomyces chattanoogensis, Streptomyces chattanoogensis L10
Appl. Environ. Microbiol.
79
3346-3354
2013
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1
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-
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8
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1
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8
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1
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2
2
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2
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
2
2
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-
738523
Viala
Posttranslational maturation o ...
Salmonella enterica subsp. enterica serovar Typhimurium
J. Bacteriol.
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4399-4405
2013
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-
-
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1
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2
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2
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2
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1
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2
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-
-
2
-
-
-
-
-
-
-
-
-
-
3
3
-
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738930
Rottier
Detection of soluble co-factor ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis ATCC 25618
J. Struct. Biol.
183
320-328
2013
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1
-
1
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-
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3
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2
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6
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1
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-
-
-
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4
-
1
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1
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-
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1
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1
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3
-
2
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1
-
-
-
-
4
-
1
-
-
-
1
-
-
-
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2
2
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-
-
722125
Owen
A functional screen for recove ...
Escherichia coli
Environ. Microbiol.
14
1198-1209
2012
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-
-
-
-
-
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5
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1
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1
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1
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-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
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723231
Leng
Sfp-type 4'-phosphopantetheiny ...
Bipolaris sorokiniana
Mol. Plant Pathol.
13
375-387
2012
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-
-
-
-
-
-
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1
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6
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1
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1
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1
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
723567
Wiemann
The Sfp-type 4-phosphopantethe ...
Fusarium fujikuroi, Fusarium fujikuroi IMI58289
PLoS ONE
7
e37519
2012
-
-
1
-
-
-
-
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1
1
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-
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8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
1
-
-
-
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-
1
1
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-
-
-
-
-
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1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
3
-
-
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723610
Leblanc
4-Phosphopantetheinyl transfer ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis BCG
PLoS Pathog.
8
e1003097
2012
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1
1
-
-
-
-
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1
1
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2
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11
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1
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-
-
-
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2
-
1
-
-
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-
1
-
1
-
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1
1
1
-
-
-
-
-
-
-
1
1
-
2
-
-
-
1
-
-
-
-
2
-
1
-
-
-
-
1
-
-
-
2
2
-
-
-
721530
Owen
Rapid and flexible biochemical ...
Bacillus subtilis, Bacillus subtilis ATCC 6633, Pseudomonas aeruginosa, Pseudomonas putida, Pseudomonas putida KT 2240
Biochem. J.
436
709-717
2011
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3
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3
6
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3
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48
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3
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-
-
-
-
5
-
-
-
-
6
-
-
-
3
6
-
6
-
-
3
3
-
-
-
6
3
6
6
-
3
-
-
-
-
-
3
-
-
-
-
5
-
-
-
-
6
-
-
-
-
-
-
-
-
6
6
721646
Dallaglio
Analysis of Streptomyces coeli ...
Streptomyces coelicolor
Biochemistry
50
5704-5717
2011
-
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1
1
4
-
-
-
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1
-
-
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2
-
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1
-
-
-
-
-
3
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
4
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
722318
Allen
Functional analysis of a mitoc ...
Aspergillus fumigatus, Aspergillus fumigatus Af293
Fungal Genet. Biol.
48
456-464
2011
-
-
1
-
-
-
-
-
2
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2
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12
-
-
1
-
-
-
-
-
4
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
2
-
2
-
-
-
-
1
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
2
2
-
-
-
723227
Velazquez-Robledo
Role of the 4-phosphopantethei ...
Trichoderma virens
Mol. Plant Microbe Interact.
24
1459-1471
2011
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-
-
-
-
-
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1
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-
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6
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
2
-
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-
723538
Nair
Two functionally distinctive p ...
Dictyostelium discoideum, Dictyostelium discoideum AX2
PLoS ONE
6
e24262
2011
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1
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-
-
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1
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3
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11
-
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1
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2
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12
1
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-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
3
-
-
-
-
2
-
2
-
-
12
2
-
-
-
-
-
-
-
-
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1
2
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712429
Strickland
Acyl carrier protein-specific ...
Homo sapiens
J. Biol. Chem.
285
1627-1633
2010
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1
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-
-
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1
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3
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1
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1
1
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-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
1
1
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-
-
-
-
-
-
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1
1
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671027
Zhou
Genetically encoded short pept ...
Bacillus subtilis, Escherichia coli
ACS Chem. Biol.
2
337-346
2007
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2
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-
-
-
-
14
-
-
-
-
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2
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-
-
-
-
-
14
-
-
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-
14
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
14
-
-
-
-
-
-
-
-
-
-
-
-
14
-
-
-
-
14
-
-
-
-
-
-
-
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674883
Gong
Neutralization of acidic resid ...
Vibrio harveyi
J. Biol. Chem.
282
4494-4503
2007
-
-
-
-
-
-
-
-
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1
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-
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-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
1
-
-
-
-
-
-
-
-
-
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-
-
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691732
Bunkoczi
Mechanism and substrate recogn ...
Homo sapiens
Chem. Biol.
14
1243-1253
2007
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1
1
1
8
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22
2
1
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1
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4
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1
1
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-
-
-
2
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-
-
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20
-
-
-
-
-
-
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-
1
1
-
1
8
-
-
-
-
22
2
1
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
20
-
-
-
-
-
-
-
-
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673661
Orikasa
A phosphopantetheinyl transfer ...
Moritella marina, Moritella marina MP-1
FEBS Lett.
580
4423-4429
2006
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1
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-
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7
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2
1
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1
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2
2
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2
1
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-
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McAllister
Acyl carrier protein synthases ...
Escherichia coli, Mycoplasma pneumoniae, Streptococcus pneumoniae
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4737-4748
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25
2
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28
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-
-
-
-
-
-
-
-
-
-
-
-
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28
-
-
4
-
-
-
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3
-
-
-
-
25
2
-
-
-
28
-
-
-
-
-
-
-
-
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674657
Huang
A dedicated phosphopantetheiny ...
Streptomyces griseus
J. Biol. Chem.
281
29660-29668
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-
-
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1
-
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2
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2
1
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2
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-
-
-
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3
1
-
-
-
2
1
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
2
-
-
2
1
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-
-
-
-
-
-
-
3
1
-
-
-
2
1
-
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1
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-
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676069
De Lay
A genome rearrangement has orp ...
Escherichia coli
Mol. Microbiol.
61
232-242
2006
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-
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1
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-
-
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1
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6
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-
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1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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1
-
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-
-
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-
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1
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-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
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661613
Cai
Functional characterization of ...
Cryptosporidium parvum
Eukaryot. Cell
4
1211-1220
2005
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1
-
-
-
-
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1
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-
-
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8
-
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-
-
-
-
-
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2
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-
-
-
-
-
-
-
-
-
-
-
-
-
1
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-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
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-
660604
Daubaras
Crystallization and preliminar ...
Staphylococcus aureus
Acta Crystallogr. Sect. D
60
773-774
2004
-
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1
-
-
-
-
-
-
-
-
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1
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1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
661153
Finking
Mutational analysis of peptidy ...
Bacillus subtilis
Biochemistry
43
8946-8956
2004
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1
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2
-
-
1
-
-
-
-
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2
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
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-
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661337
Gilbert
Anthranilate 4H-oxazol-5-ones: ...
Bacillus subtilis
Bioorg. Med. Chem. Lett.
14
37-41
2004
-
-
-
-
-
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12
-
-
-
-
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1
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-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
12
-
-
-
-
-
-
-
12
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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661479
Weissman
Identification of a phosphopan ...
Saccharopolyspora erythraea
ChemBioChem
5
116-125
2004
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1
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-
-
-
-
-
-
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5
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-
-
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1
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-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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1
-
-
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-
-
-
-
-
-
-
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662789
Barekzi
Genetic characterization of pc ...
Pseudomonas aeruginosa
Microbiology
150
795-803
2004
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1
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1
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5
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1
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2
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-
-
-
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1
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Cloning, expression, and chara ...
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1
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Reed
The type I rat fatty acid synt ...
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Org. Biomol. Chem.
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2003
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645413
Chopra
Expression, purification, crys ...
Mycobacterium tuberculosis
Acta Crystallogr. Sect. D
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2002
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645414
Mofid
Recognition of hybrid peptidyl ...
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2002
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645415
Mootz
Functional characterization of ...
Aspergillus nidulans, Aspergillus nidulans FGSC4, Bacillus subtilis, Brevibacillus brevis, no activity in Saccharomyces cerevisiae, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Schizosaccharomyces pombe ATCC 24843
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Finking
Characterization of a new type ...
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2002
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645410
Flaman
Site-directed mutagenesis of a ...
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J. Biol. Chem.
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2001
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Gaitatzis
The mtaA gene of the myxothiaz ...
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2001
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645412
Sanchez
Cloning and characterization o ...
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2001
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645406
Chirgadze
Crystal structure of Streptoco ...
Streptomyces pneumoniae
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2000
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645407
Flugel
Holo-(acyl carrier protein) sy ...
Escherichia coli, no activity in Escherichia coli, no activity in Escherichia coli MP4
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2000
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645408
McAllister
Biochemical and molecular anal ...
Streptomyces pneumoniae
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2000
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645409
Parris
Crystal structures of substrat ...
Bacillus subtilis
Structure Fold. Des.
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2000
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645404
Quadri
Characterization of Sfp, a Bac ...
Bacillus subtilis
Biochemistry
37
1585-1595
1998
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8
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13
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8
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1
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13
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6
1
1
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645405
Stuible
A novel phosphopantetheine:pro ...
Saccharomyces cerevisiae
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1998
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1
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1
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1
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645401
Gehring
Ability of Streptomyces spp. a ...
Escherichia coli
Chem. Biol.
4
17-24
1997
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5
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5
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3
5
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-
10
-
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5
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645402
Lambalot
Holo-[acyl-carrier-protein] sy ...
Escherichia coli
Methods Enzymol.
279
254-262
1997
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1
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1
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1
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1
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1
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2
1
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1
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1
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645403
Stuible
Identification, isolation and ...
Corynebacterium ammoniagenes
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248
481-487
1997
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1
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645400
Revill
Relationships between fatty ac ...
Streptomyces coelicolor, Streptomyces coelicolor A3(2)
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5660-5667
1996
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6
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6
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645399
Lambalot
Cloning, overproduction, and c ...
Escherichia coli
J. Biol. Chem.
270
24658-24661
1995
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1
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1
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1
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2
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1
1
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645398
Yang
Acyl carrier protein (ACP) imp ...
Spinacia oleracea, Spinacia oleracea Melody
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743-750
1994
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Elhussein
Plant holo-(acyl carrier prote ...
Ricinus communis, Spinacia oleracea
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252
39-45
1988
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645396
Werkmeister
Coenzyme A: fatty acid synthet ...
Saccharomyces cerevisiae
Biochem. Biophys. Res. Commun.
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483-490
1980
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1
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645395
Prescott
-
Acyl carrier protein synthetas ...
Escherichia coli, Escherichia coli B / ATCC 11303
Methods Enzymol.
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95-101
1975
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Prescott
Acyl carrier protein ...
Clostridium kluyveri, Escherichia coli, Rattus norvegicus, Saccharomyces cerevisiae
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Elovson
Acyl carrier protein. X. Acyl ...
Escherichia coli, Escherichia coli B / ATCC 11303
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