BRENDA - Enzyme Database show
show all sequences of 2.7.8.7

Structure, biochemistry, and inhibition of essential 4'-phosphopantetheinyl transferases from two species of Mycobacteria

Vickery, C.R.; Kosa, N.M.; Casavant, E.P.; Duan, S.; Noel, J.P.; Burkart, M.D.; ACS Chem. Biol. 9, 1939-1944 (2014)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
recombinant expression of enzyme PptT as maltose-binding-protein fusion protein
Mycobacterium tuberculosis
recombinant expression of enzyme PptT as maltose-binding-protein fusion protein
Mycobacterium ulcerans
Crystallization (Commentary)
Crystallization
Organism
purified recombinant MBP-fusion enzyme, X-ray diffraction structure determination and analysis at 1.59 A resolution
Mycobacterium tuberculosis
purified recombinant MBP-fusion enzyme, X-ray diffraction structure determination and analysis at 1.65 A resolution
Mycobacterium ulcerans
Engineering
Amino acid exchange
Commentary
Organism
D114N
site-directed mutagenesis, the mutation eliminates the negative charge of one of the putative Mg2+ ligands, also abolishes phosphopantetheinylation activity of PptT, inactive mutant
Mycobacterium tuberculosis
E109D
site-directed mutagenesis, inactive mutant, no measurable catalytic activity
Mycobacterium tuberculosis
E116Q
site-directed mutagenesis, the mutant shows 500fold reduced activity compared to the wild-type enzyme
Mycobacterium tuberculosis
E157Q
site-directed mutagenesis, removal of the negative charge associated with the putative Mg2+ ligand/general base abolishes phosphopantetheinylation activity, inactive mutant
Mycobacterium tuberculosis
R48A
site-directed mutagenesis, the mutation disrupts 3'-phosphate binding, the activity is reduced by 20fold compared to the wild-type enzyme
Mycobacterium tuberculosis
R56A
site-directed mutagenesis, the mutation disrupts 3'-phosphate binding, the activity is reduced by 100fold compared to the wild-type enzyme
Mycobacterium tuberculosis
Inhibitors
Inhibitors
Commentary
Organism
Structure
2'-deoxy-adenosine-3',5'-bisphosphate
-
Mycobacterium tuberculosis
6-nitroso-benzopyrone
-
Mycobacterium tuberculosis
adenosine-3',5'-bisphosphate
-
Mycobacterium tuberculosis
Bay 11-7085
-
Mycobacterium tuberculosis
Calmidazolium
-
Mycobacterium tuberculosis
CoA
-
Mycobacterium tuberculosis
guanidinyl-naltrindole
-
Mycobacterium tuberculosis
additional information
no or poor inhibition by Bay 11-7085, benserazide, (-)-ephedrine hemisulfate, and mitoxantrone
Mycobacterium tuberculosis
PD 404182
-
Mycobacterium tuberculosis
sanguinarine
-
Mycobacterium tuberculosis
SCH-202676
-
Mycobacterium tuberculosis
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Mycobacterium tuberculosis
Mg2+
required
Mycobacterium ulcerans
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein]
Mycobacterium tuberculosis
-
adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
-
?
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein]
Mycobacterium ulcerans
-
adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
-
?
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein]
Mycobacterium tuberculosis H37Rv
-
adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mycobacterium tuberculosis
P9WQD3
strain H37Rv
-
Mycobacterium tuberculosis H37Rv
P9WQD3
strain H37Rv
-
Mycobacterium ulcerans
A0PU93
strain Agy99
-
Purification (Commentary)
Commentary
Organism
recombinant MBP-fusion enzyme
Mycobacterium tuberculosis
recombinant MBP-fusion enzyme
Mycobacterium ulcerans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein]
-
737325
Mycobacterium tuberculosis
adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
-
-
?
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein]
-
737325
Mycobacterium ulcerans
adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
-
-
?
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein]
-
737325
Mycobacterium tuberculosis H37Rv
adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
-
-
?
Subunits
Subunits
Commentary
Organism
More
enzyme PptT possesses a pseudodimeric fold characteristic of Sfp-type PPTases, three-dimensional structure comparisons, modeling, overview
Mycobacterium tuberculosis
More
enzyme MuPPT possesses a pseudodimeric fold characteristic of Sfp-type PPTases, three-dimensional structure comparisons, modeling, overview
Mycobacterium ulcerans
IC50 Value
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.0005
-
with substrate MAS, recombinant enzyme, pH and temperature not specified in the publication
Mycobacterium tuberculosis
SCH-202676
0.00078
-
with substrate VibB, recombinant enzyme, pH and temperature not specified in the publication
Mycobacterium tuberculosis
adenosine-3',5'-bisphosphate
0.0008
-
with substrate VibB, recombinant enzyme, pH and temperature not specified in the publication
Mycobacterium tuberculosis
SCH-202676
0.0011
-
with substrate MAS, recombinant enzyme, pH and temperature not specified in the publication
Mycobacterium tuberculosis
CoA
0.0016
-
with substrate MAS, recombinant enzyme, pH and temperature not specified in the publication
Mycobacterium tuberculosis
adenosine-3',5'-bisphosphate
0.002
-
with substrate VibB, recombinant enzyme, pH and temperature not specified in the publication
Mycobacterium tuberculosis
Calmidazolium
0.0047
-
with substrate VibB, recombinant enzyme, pH and temperature not specified in the publication
Mycobacterium tuberculosis
CoA
0.0049
-
with substrate MAS, recombinant enzyme, pH and temperature not specified in the publication
Mycobacterium tuberculosis
Calmidazolium
0.0049
-
with substrate MAS, recombinant enzyme, pH and temperature not specified in the publication
Mycobacterium tuberculosis
sanguinarine
0.0071
-
with substrate VibB, recombinant enzyme, pH and temperature not specified in the publication
Mycobacterium tuberculosis
PD 404182
0.0074
-
with substrate MAS, recombinant enzyme, pH and temperature not specified in the publication
Mycobacterium tuberculosis
2'-deoxy-adenosine-3',5'-bisphosphate
0.0085
-
with substrate VibB, recombinant enzyme, pH and temperature not specified in the publication
Mycobacterium tuberculosis
2'-deoxy-adenosine-3',5'-bisphosphate
0.012
-
with substrate MAS, recombinant enzyme, pH and temperature not specified in the publication
Mycobacterium tuberculosis
guanidinyl-naltrindole
0.017
-
with substrate VibB, recombinant enzyme, pH and temperature not specified in the publication
Mycobacterium tuberculosis
6-nitroso-benzopyrone
0.019
-
with substrate VibB, recombinant enzyme, pH and temperature not specified in the publication
Mycobacterium tuberculosis
guanidinyl-naltrindole
0.019
-
with substrate MAS, recombinant enzyme, pH and temperature not specified in the publication
Mycobacterium tuberculosis
PD 404182
0.022
-
with substrate VibB, recombinant enzyme, pH and temperature not specified in the publication
Mycobacterium tuberculosis
sanguinarine
0.024
-
with substrate MAS, recombinant enzyme, pH and temperature not specified in the publication
Mycobacterium tuberculosis
6-nitroso-benzopyrone
0.03
-
with substrate MAS, recombinant enzyme, pH and temperature not specified in the publication
Mycobacterium tuberculosis
Bay 11-7085
Cloned(Commentary) (protein specific)
Commentary
Organism
recombinant expression of enzyme PptT as maltose-binding-protein fusion protein
Mycobacterium tuberculosis
recombinant expression of enzyme PptT as maltose-binding-protein fusion protein
Mycobacterium ulcerans
Crystallization (Commentary) (protein specific)
Crystallization
Organism
purified recombinant MBP-fusion enzyme, X-ray diffraction structure determination and analysis at 1.59 A resolution
Mycobacterium tuberculosis
purified recombinant MBP-fusion enzyme, X-ray diffraction structure determination and analysis at 1.65 A resolution
Mycobacterium ulcerans
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
D114N
site-directed mutagenesis, the mutation eliminates the negative charge of one of the putative Mg2+ ligands, also abolishes phosphopantetheinylation activity of PptT, inactive mutant
Mycobacterium tuberculosis
E109D
site-directed mutagenesis, inactive mutant, no measurable catalytic activity
Mycobacterium tuberculosis
E116Q
site-directed mutagenesis, the mutant shows 500fold reduced activity compared to the wild-type enzyme
Mycobacterium tuberculosis
E157Q
site-directed mutagenesis, removal of the negative charge associated with the putative Mg2+ ligand/general base abolishes phosphopantetheinylation activity, inactive mutant
Mycobacterium tuberculosis
R48A
site-directed mutagenesis, the mutation disrupts 3'-phosphate binding, the activity is reduced by 20fold compared to the wild-type enzyme
Mycobacterium tuberculosis
R56A
site-directed mutagenesis, the mutation disrupts 3'-phosphate binding, the activity is reduced by 100fold compared to the wild-type enzyme
Mycobacterium tuberculosis
IC50 Value (protein specific)
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.0005
-
with substrate MAS, recombinant enzyme, pH and temperature not specified in the publication
Mycobacterium tuberculosis
SCH-202676
0.00078
-
with substrate VibB, recombinant enzyme, pH and temperature not specified in the publication
Mycobacterium tuberculosis
adenosine-3',5'-bisphosphate
0.0008
-
with substrate VibB, recombinant enzyme, pH and temperature not specified in the publication
Mycobacterium tuberculosis
SCH-202676
0.0011
-
with substrate MAS, recombinant enzyme, pH and temperature not specified in the publication
Mycobacterium tuberculosis
CoA
0.0016
-
with substrate MAS, recombinant enzyme, pH and temperature not specified in the publication
Mycobacterium tuberculosis
adenosine-3',5'-bisphosphate
0.002
-
with substrate VibB, recombinant enzyme, pH and temperature not specified in the publication
Mycobacterium tuberculosis
Calmidazolium
0.0047
-
with substrate VibB, recombinant enzyme, pH and temperature not specified in the publication
Mycobacterium tuberculosis
CoA
0.0049
-
with substrate MAS, recombinant enzyme, pH and temperature not specified in the publication
Mycobacterium tuberculosis
Calmidazolium
0.0049
-
with substrate MAS, recombinant enzyme, pH and temperature not specified in the publication
Mycobacterium tuberculosis
sanguinarine
0.0071
-
with substrate VibB, recombinant enzyme, pH and temperature not specified in the publication
Mycobacterium tuberculosis
PD 404182
0.0074
-
with substrate MAS, recombinant enzyme, pH and temperature not specified in the publication
Mycobacterium tuberculosis
2'-deoxy-adenosine-3',5'-bisphosphate
0.0085
-
with substrate VibB, recombinant enzyme, pH and temperature not specified in the publication
Mycobacterium tuberculosis
2'-deoxy-adenosine-3',5'-bisphosphate
0.012
-
with substrate MAS, recombinant enzyme, pH and temperature not specified in the publication
Mycobacterium tuberculosis
guanidinyl-naltrindole
0.017
-
with substrate VibB, recombinant enzyme, pH and temperature not specified in the publication
Mycobacterium tuberculosis
6-nitroso-benzopyrone
0.019
-
with substrate VibB, recombinant enzyme, pH and temperature not specified in the publication
Mycobacterium tuberculosis
guanidinyl-naltrindole
0.019
-
with substrate MAS, recombinant enzyme, pH and temperature not specified in the publication
Mycobacterium tuberculosis
PD 404182
0.022
-
with substrate VibB, recombinant enzyme, pH and temperature not specified in the publication
Mycobacterium tuberculosis
sanguinarine
0.024
-
with substrate MAS, recombinant enzyme, pH and temperature not specified in the publication
Mycobacterium tuberculosis
6-nitroso-benzopyrone
0.03
-
with substrate MAS, recombinant enzyme, pH and temperature not specified in the publication
Mycobacterium tuberculosis
Bay 11-7085
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2'-deoxy-adenosine-3',5'-bisphosphate
-
Mycobacterium tuberculosis
6-nitroso-benzopyrone
-
Mycobacterium tuberculosis
adenosine-3',5'-bisphosphate
-
Mycobacterium tuberculosis
Bay 11-7085
-
Mycobacterium tuberculosis
Calmidazolium
-
Mycobacterium tuberculosis
CoA
-
Mycobacterium tuberculosis
guanidinyl-naltrindole
-
Mycobacterium tuberculosis
additional information
no or poor inhibition by Bay 11-7085, benserazide, (-)-ephedrine hemisulfate, and mitoxantrone
Mycobacterium tuberculosis
PD 404182
-
Mycobacterium tuberculosis
sanguinarine
-
Mycobacterium tuberculosis
SCH-202676
-
Mycobacterium tuberculosis
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Mycobacterium tuberculosis
Mg2+
required
Mycobacterium ulcerans
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein]
Mycobacterium tuberculosis
-
adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
-
?
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein]
Mycobacterium ulcerans
-
adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
-
?
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein]
Mycobacterium tuberculosis H37Rv
-
adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant MBP-fusion enzyme
Mycobacterium tuberculosis
recombinant MBP-fusion enzyme
Mycobacterium ulcerans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein]
-
737325
Mycobacterium tuberculosis
adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
-
-
?
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein]
-
737325
Mycobacterium ulcerans
adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
-
-
?
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein]
-
737325
Mycobacterium tuberculosis H37Rv
adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
More
enzyme PptT possesses a pseudodimeric fold characteristic of Sfp-type PPTases, three-dimensional structure comparisons, modeling, overview
Mycobacterium tuberculosis
More
enzyme MuPPT possesses a pseudodimeric fold characteristic of Sfp-type PPTases, three-dimensional structure comparisons, modeling, overview
Mycobacterium ulcerans
General Information
General Information
Commentary
Organism
additional information
analysis and comparisons of cofactor binding and active site composition, CoA binding pocket, catalytic mechanism of PptT, overview. While important for CoA binding, Arg48 and Arg56 likely play very small roles in catalysis
Mycobacterium tuberculosis
additional information
analysis and comparisons of cofactor binding and active site composition, CoA binding pocket, overview
Mycobacterium ulcerans
physiological function
4'-phosphopantetheinyl transferase post-translationally modifies carrier proteins with a phosphopantetheine moiety, an essential reaction. In Mycobacteria, the Sfp-type PPTase activates pathways necessary for the biosynthesis of cell wall components and small molecule virulence factors
Mycobacterium tuberculosis
physiological function
4'-phosphopantetheinyl transferase post-translationally modifies carrier proteins with a phosphopantetheine moiety, an essential reaction. In Mycobacteria, the Sfp-type PPTase activates pathways necessary for the biosynthesis of cell wall components and small molecule virulence factors
Mycobacterium ulcerans
General Information (protein specific)
General Information
Commentary
Organism
additional information
analysis and comparisons of cofactor binding and active site composition, CoA binding pocket, catalytic mechanism of PptT, overview. While important for CoA binding, Arg48 and Arg56 likely play very small roles in catalysis
Mycobacterium tuberculosis
additional information
analysis and comparisons of cofactor binding and active site composition, CoA binding pocket, overview
Mycobacterium ulcerans
physiological function
4'-phosphopantetheinyl transferase post-translationally modifies carrier proteins with a phosphopantetheine moiety, an essential reaction. In Mycobacteria, the Sfp-type PPTase activates pathways necessary for the biosynthesis of cell wall components and small molecule virulence factors
Mycobacterium tuberculosis
physiological function
4'-phosphopantetheinyl transferase post-translationally modifies carrier proteins with a phosphopantetheine moiety, an essential reaction. In Mycobacteria, the Sfp-type PPTase activates pathways necessary for the biosynthesis of cell wall components and small molecule virulence factors
Mycobacterium ulcerans
Other publictions for EC 2.7.8.7
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737568
Wang
The substrate promiscuity of a ...
Streptomyces chattanoogensis, Streptomyces chattanoogensis L10
Arch. Microbiol.
198
193-197
2016
-
1
1
-
-
-
-
-
-
1
-
2
-
4
-
-
1
-
-
-
-
-
6
-
1
-
-
-
1
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
1
-
-
-
-
6
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
739683
Wang
Characterization of discrete p ...
Streptomyces tsukubensis, Streptomyces tsukubensis L19
Sci. Rep.
6
24255
2016
-
-
1
-
-
-
-
-
-
-
-
10
-
12
-
-
1
-
-
-
-
-
20
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
10
-
-
-
5
-
-
-
-
20
-
-
-
-
-
-
-
-
-
-
3
12
-
-
-
737715
Kumar
The structure of the holo-acyl ...
Bacillus subtilis 168, Bacillus subtilis, Leishmania major
Biochemistry
54
5632-5645
2015
-
-
2
-
-
-
2
11
-
2
-
3
-
5
-
-
2
-
-
-
-
-
7
-
2
-
-
10
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
2
-
11
-
2
-
3
-
-
-
2
-
-
-
-
7
-
2
-
-
10
2
-
-
-
-
-
-
-
-
-
738868
Kim
Characterization of NpgA, a 4' ...
Aspergillus nidulans, Aspergillus nidulans SNT611
J. Microbiol.
53
21-31
2015
-
-
1
-
1
-
-
-
-
1
1
2
-
5
-
-
1
-
-
5
-
-
4
2
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
1
2
-
-
-
1
-
5
-
-
4
2
1
-
-
-
1
-
-
-
-
3
3
-
-
-
739319
Guan
A phosphopantetheinyl transfer ...
Arabidopsis thaliana
Plant J.
84
718-732
2015
-
-
1
-
1
-
-
-
2
-
-
1
-
4
-
-
-
-
-
4
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
2
-
-
-
-
-
2
-
-
1
-
-
-
-
-
4
-
-
3
1
-
-
-
-
-
-
-
-
-
3
3
-
-
-
739435
Dobb
Characterisation of the Candid ...
Candida albicans
PLoS ONE
10
e0143770
2015
-
1
1
-
1
-
-
-
-
1
-
1
-
3
-
-
1
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
1
1
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
737325
Vickery
Structure, biochemistry, and i ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv, Mycobacterium ulcerans
ACS Chem. Biol.
9
1939-1944
2014
-
-
2
2
6
-
11
-
-
2
-
3
-
6
-
-
2
-
-
-
-
-
3
2
-
-
-
-
-
-
-
-
-
-
19
-
-
2
-
2
6
-
19
11
-
-
-
2
-
3
-
-
-
2
-
-
-
-
3
2
-
-
-
-
-
-
-
-
-
4
4
-
-
-
738470
Kosa
Fluorescent techniques for dis ...
Homo sapiens
J. Antibiot.
67
113-120
2014
-
-
1
-
-
-
-
-
-
1
-
1
-
3
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
738932
Jung
Crystal structure of the essen ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis ATCC 25618
J. Struct. Biol.
188
274-278
2014
-
1
1
1
-
-
-
-
-
1
-
2
-
5
-
-
1
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
1
-
2
-
-
-
1
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
739157
Beld
The phosphopantetheinyl transf ...
Bacillus anthracis, Bacillus licheniformis, Bacillus subtilis 168, Bacillus subtilis, Brevibacillus brevis, Burkholderia cenocepacia, Burkholderia pseudomallei, Burkholderia pseudomallei K96243, Escherichia coli, Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv, Pseudomonas aeruginosa, Pseudomonas aeruginosa DSM 22644, Serratia marcescens subsp. marcescens, Serratia marcescens subsp. marcescens Db11, Streptomyces coelicolor, Streptomyces coelicolor ATCC BAA-471, Vibrio anguillarum, Vibrio anguillarum ATCC 68554, Vibrio cholerae, Xanthomonas albilineans, Xanthomonas albilineans GPE PC73, Yersinia pestis
Nat. Prod. Rep.
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2014
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2
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30
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5
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10
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1
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4
28
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52
7
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1
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1
31
42
1
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739377
Contreras-Cornejo
-
The 4-phosphopantetheinyl tran ...
Trichoderma virens
Plant Soil
379
261-274
2014
-
-
-
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4
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2
4
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721361
Jiang
Improvement of natamycin produ ...
Streptomyces chattanoogensis, Streptomyces chattanoogensis L10
Appl. Environ. Microbiol.
79
3346-3354
2013
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1
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8
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1
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8
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2
2
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2
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8
-
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2
2
-
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-
738523
Viala
Posttranslational maturation o ...
Salmonella enterica subsp. enterica serovar Typhimurium
J. Bacteriol.
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4399-4405
2013
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-
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1
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2
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2
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2
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3
3
-
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738930
Rottier
Detection of soluble co-factor ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis ATCC 25618
J. Struct. Biol.
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320-328
2013
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1
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1
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6
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1
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4
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1
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1
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1
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1
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2
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1
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4
-
1
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1
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2
2
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722125
Owen
A functional screen for recove ...
Escherichia coli
Environ. Microbiol.
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2012
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5
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1
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-
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1
-
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-
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-
-
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1
1
-
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723231
Leng
Sfp-type 4'-phosphopantetheiny ...
Bipolaris sorokiniana
Mol. Plant Pathol.
13
375-387
2012
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-
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1
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6
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1
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1
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-
-
-
-
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2
2
-
-
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723567
Wiemann
The Sfp-type 4-phosphopantethe ...
Fusarium fujikuroi, Fusarium fujikuroi IMI58289
PLoS ONE
7
e37519
2012
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1
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1
1
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8
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1
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1
1
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1
1
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-
-
-
-
-
-
-
-
-
-
-
-
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3
3
-
-
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723610
Leblanc
4-Phosphopantetheinyl transfer ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis BCG
PLoS Pathog.
8
e1003097
2012
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1
1
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-
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1
1
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2
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11
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1
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2
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1
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1
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1
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1
1
1
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1
1
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2
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1
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2
-
1
-
-
-
-
1
-
-
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2
2
-
-
-
721530
Owen
Rapid and flexible biochemical ...
Bacillus subtilis, Bacillus subtilis ATCC 6633, Pseudomonas aeruginosa, Pseudomonas putida, Pseudomonas putida KT 2240
Biochem. J.
436
709-717
2011
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3
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3
6
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3
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3
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5
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6
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3
6
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6
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3
3
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6
3
6
6
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3
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-
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3
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-
-
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5
-
-
-
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6
-
-
-
-
-
-
-
-
6
6
721646
Dallaglio
Analysis of Streptomyces coeli ...
Streptomyces coelicolor
Biochemistry
50
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2011
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1
1
4
-
-
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1
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2
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1
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3
1
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1
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1
1
1
4
-
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1
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1
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-
-
-
3
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
722318
Allen
Functional analysis of a mitoc ...
Aspergillus fumigatus, Aspergillus fumigatus Af293
Fungal Genet. Biol.
48
456-464
2011
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1
-
-
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2
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2
-
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12
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1
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4
1
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1
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1
1
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-
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2
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2
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1
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-
-
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4
1
-
-
-
-
-
-
-
-
-
2
2
-
-
-
723227
Velazquez-Robledo
Role of the 4-phosphopantethei ...
Trichoderma virens
Mol. Plant Microbe Interact.
24
1459-1471
2011
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-
-
-
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1
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6
-
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1
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1
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1
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-
-
-
-
-
-
-
-
-
-
-
-
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2
2
-
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-
723538
Nair
Two functionally distinctive p ...
Dictyostelium discoideum, Dictyostelium discoideum AX2
PLoS ONE
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2011
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1
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1
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3
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11
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1
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2
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12
1
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-
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2
-
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-
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2
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3
-
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2
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2
-
-
12
2
-
-
-
-
-
-
-
-
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1
2
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712429
Strickland
Acyl carrier protein-specific ...
Homo sapiens
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2010
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1
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-
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1
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1
1
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1
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1
1
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-
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1
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1
-
-
1
1
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-
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-
-
-
-
-
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1
1
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671027
Zhou
Genetically encoded short pept ...
Bacillus subtilis, Escherichia coli
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2007
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2
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-
-
-
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14
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-
-
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2
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-
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14
-
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-
14
-
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2
-
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-
-
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-
14
-
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-
-
-
-
-
14
-
-
-
-
14
-
-
-
-
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-
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674883
Gong
Neutralization of acidic resid ...
Vibrio harveyi
J. Biol. Chem.
282
4494-4503
2007
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-
-
-
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-
-
-
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1
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1
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-
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-
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-
1
-
-
-
-
-
-
-
-
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691732
Bunkoczi
Mechanism and substrate recogn ...
Homo sapiens
Chem. Biol.
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2007
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1
1
1
8
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22
2
1
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1
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4
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1
1
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-
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2
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20
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-
-
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1
1
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1
8
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22
2
1
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1
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1
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-
-
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2
-
-
-
-
20
-
-
-
-
-
-
-
-
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673661
Orikasa
A phosphopantetheinyl transfer ...
Moritella marina, Moritella marina MP-1
FEBS Lett.
580
4423-4429
2006
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1
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-
-
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2
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1
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2
1
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McAllister
Acyl carrier protein synthases ...
Escherichia coli, Mycoplasma pneumoniae, Streptococcus pneumoniae
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2
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28
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-
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-
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-
-
-
-
-
-
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28
-
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4
-
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-
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3
-
-
-
-
25
2
-
-
-
28
-
-
-
-
-
-
-
-
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-
674657
Huang
A dedicated phosphopantetheiny ...
Streptomyces griseus
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281
29660-29668
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-
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1
-
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2
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2
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3
1
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2
1
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1
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-
-
-
-
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1
-
-
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2
-
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2
1
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-
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-
-
-
-
-
3
1
-
-
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2
1
-
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1
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-
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676069
De Lay
A genome rearrangement has orp ...
Escherichia coli
Mol. Microbiol.
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232-242
2006
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-
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1
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-
-
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1
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6
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1
-
-
-
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-
-
-
-
-
-
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-
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-
-
-
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1
-
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-
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-
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1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
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661613
Cai
Functional characterization of ...
Cryptosporidium parvum
Eukaryot. Cell
4
1211-1220
2005
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1
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-
-
-
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1
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-
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8
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-
-
-
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2
-
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-
-
-
-
-
-
-
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1
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-
-
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-
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1
-
-
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-
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-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
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660604
Daubaras
Crystallization and preliminar ...
Staphylococcus aureus
Acta Crystallogr. Sect. D
60
773-774
2004
-
-
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1
-
-
-
-
-
-
-
-
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1
-
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1
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
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-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
661153
Finking
Mutational analysis of peptidy ...
Bacillus subtilis
Biochemistry
43
8946-8956
2004
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1
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2
-
-
1
-
-
-
-
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2
-
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1
-
-
-
-
-
-
-
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-
-
1
-
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-
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-
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-
1
-
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2
-
-
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-
1
-
-
-
-
-
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1
-
-
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-
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-
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1
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661337
Gilbert
Anthranilate 4H-oxazol-5-ones: ...
Bacillus subtilis
Bioorg. Med. Chem. Lett.
14
37-41
2004
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-
-
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12
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12
-
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12
12
-
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-
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661479
Weissman
Identification of a phosphopan ...
Saccharopolyspora erythraea
ChemBioChem
5
116-125
2004
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1
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5
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1
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1
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662789
Barekzi
Genetic characterization of pc ...
Pseudomonas aeruginosa
Microbiology
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Cloning, expression, and chara ...
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1
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Reed
The type I rat fatty acid synt ...
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645413
Chopra
Expression, purification, crys ...
Mycobacterium tuberculosis
Acta Crystallogr. Sect. D
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2002
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Mofid
Recognition of hybrid peptidyl ...
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645415
Mootz
Functional characterization of ...
Aspergillus nidulans, Aspergillus nidulans FGSC4, Bacillus subtilis, Brevibacillus brevis, no activity in Saccharomyces cerevisiae, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Schizosaccharomyces pombe ATCC 24843
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Finking
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Flaman
Site-directed mutagenesis of a ...
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J. Biol. Chem.
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2001
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Gaitatzis
The mtaA gene of the myxothiaz ...
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2001
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645412
Sanchez
Cloning and characterization o ...
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2001
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645406
Chirgadze
Crystal structure of Streptoco ...
Streptomyces pneumoniae
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2000
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645407
Flugel
Holo-(acyl carrier protein) sy ...
Escherichia coli, no activity in Escherichia coli, no activity in Escherichia coli MP4
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2000
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645408
McAllister
Biochemical and molecular anal ...
Streptomyces pneumoniae
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275
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2000
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645409
Parris
Crystal structures of substrat ...
Bacillus subtilis
Structure Fold. Des.
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2000
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645404
Quadri
Characterization of Sfp, a Bac ...
Bacillus subtilis
Biochemistry
37
1585-1595
1998
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1
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8
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5
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13
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13
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6
1
1
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645405
Stuible
A novel phosphopantetheine:pro ...
Saccharomyces cerevisiae
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1998
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645401
Gehring
Ability of Streptomyces spp. a ...
Escherichia coli
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4
17-24
1997
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5
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5
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10
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5
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645402
Lambalot
Holo-[acyl-carrier-protein] sy ...
Escherichia coli
Methods Enzymol.
279
254-262
1997
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645403
Stuible
Identification, isolation and ...
Corynebacterium ammoniagenes
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481-487
1997
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645400
Revill
Relationships between fatty ac ...
Streptomyces coelicolor, Streptomyces coelicolor A3(2)
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5660-5667
1996
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6
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645399
Lambalot
Cloning, overproduction, and c ...
Escherichia coli
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270
24658-24661
1995
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1
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1
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2
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1
1
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645398
Yang
Acyl carrier protein (ACP) imp ...
Spinacia oleracea, Spinacia oleracea Melody
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743-750
1994
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Elhussein
Plant holo-(acyl carrier prote ...
Ricinus communis, Spinacia oleracea
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252
39-45
1988
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1
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Werkmeister
Coenzyme A: fatty acid synthet ...
Saccharomyces cerevisiae
Biochem. Biophys. Res. Commun.
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483-490
1980
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1
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645395
Prescott
-
Acyl carrier protein synthetas ...
Escherichia coli, Escherichia coli B / ATCC 11303
Methods Enzymol.
35B
95-101
1975
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486864
Prescott
Acyl carrier protein ...
Clostridium kluyveri, Escherichia coli, Rattus norvegicus, Saccharomyces cerevisiae
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269-311
1972
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645393
Elovson
Acyl carrier protein. X. Acyl ...
Escherichia coli, Escherichia coli B / ATCC 11303
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243
3603-3611
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