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Literature summary for 2.7.8.7 extracted from
- Acyl carrier protein synthetase (1975), Methods Enzymol., 35B, 95-101.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| stable when CoA is present at half-saturating concentrations | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| apo-acyl-carrier protein | substrate inhibition | Escherichia coli |  |
| additional information | no substrate inhibition by apo-peptide(1--74) | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00055 | - |
apo-acyl-carrier protein | pH 8.0, 33°C | Escherichia coli | |
| 0.002 | - |
apo-peptide(1-->74) | - |
Escherichia coli | |
| 0.15 | - |
CoA | pH 8.0, 33°C | Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Escherichia coli | |
| Mn2+ | - |
Escherichia coli | |
| additional information | Ca2+, Fe2+, Co2+ and Zn2+ are ineffective, inhibitory or both, at intermediate and high concentrations | Escherichia coli |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
gel filtration, sucrose density gradient sedimentation | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CoA + apo-[acyl-carrier protein] | Escherichia coli | - |
adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
r | |
| CoA + apo-[acyl-carrier protein] | Escherichia coli B / ATCC 11303 | - |
adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Escherichia coli B / ATCC 11303 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0079 | - |
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -196°C, stored in liquid nitrogen, enzyme maintains full activity for at least 1 year | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CoA + apo-[acyl-carrier protein] | - |
Escherichia coli | adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
r | |
| CoA + apo-[acyl-carrier protein] | - |
Escherichia coli B / ATCC 11303 | adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
r | |
| CoA-[4'-phosphopantetheine] + apo-peptide(1->74) | - |
Escherichia coli | ? | - |
? | |
| CoA-[4'-phosphopantetheine] + apo-peptide(1->74) | - |
Escherichia coli B / ATCC 11303 | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 9 | - |
Escherichia coli |
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Release 2023.1 (January 2023)
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