KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0004 | - |
apo-acyl-carrier protein | pH 8.0, 33°C | Escherichia coli | |
0.002 | - |
apo-peptide(1-->74) | pH 8.0, 33°C | Escherichia coli | |
0.15 | - |
CoA | pH 8.0, 33°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | requires Mg2+ or Mn2+ for activity, Km for Mg2+ 3.0 mM | Escherichia coli | |
Mn2+ | requires Mn2+ or Mg2+ for activity | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CoA + apo-[acyl-carrier protein] | Escherichia coli | ACP serves as cofactor in the biosynthesis of fatty acids and the biosynthesis of complex lipids | adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
r | |
CoA + apo-[acyl-carrier protein] | Escherichia coli B / ATCC 11303 | ACP serves as cofactor in the biosynthesis of fatty acids and the biosynthesis of complex lipids | adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli B / ATCC 11303 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
specific activity 88.0 units/mg protein | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CoA + apo-[acyl-carrier protein] | transfers 4'-phosphopantetheine from reduced coenzyme A to acyl carrier proteon apoprotein | Escherichia coli | adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
r | |
CoA + apo-[acyl-carrier protein] | ACP serves as cofactor in the biosynthesis of fatty acids and the biosynthesis of complex lipids | Escherichia coli | adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
r | |
CoA + apo-[acyl-carrier protein] | transfers 4'-phosphopantetheine from reduced coenzyme A to acyl carrier proteon apoprotein | Escherichia coli B / ATCC 11303 | adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
r | |
CoA + apo-[acyl-carrier protein] | ACP serves as cofactor in the biosynthesis of fatty acids and the biosynthesis of complex lipids | Escherichia coli B / ATCC 11303 | adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
r | |
additional information | specificity of the holo-ACP synthetase is not examined in detail, only CoA is the donor of the 4'-phosphopantetheine moiety, dephospho-CoA is essentially inactive | Escherichia coli | ? | - |
? | |
additional information | specificity of the holo-ACP synthetase is not examined in detail, only CoA is the donor of the 4'-phosphopantetheine moiety, dephospho-CoA is essentially inactive | Escherichia coli B / ATCC 11303 | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9 | - |
Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 11 | about 20% of activity maximum at pH 6.5, about 30% of activity maximum at pH 11.0 | Escherichia coli |