Any feedback?
Literature summary for 2.7.8.35 extracted from
- Fluorescence-based assay for polyprenyl phosphate-GlcNAc-1-phosphate transferase (WecA) and identification of novel antimycobacterial WecA inhibitors (2016), Anal. Biochem., 512, 78-90.
Application
| Application | Comment | Organism |
|---|---|---|
| drug development | the enzyme is a target for development of drugs | Mycobacterium tuberculosis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene wecA, recombinant overexpression in Escherichia coli strain BL21 | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| DMSO | 50% inhibition at 10% v/v, no inhibition at 2.86% v/v | Escherichia coli |  |
| DMSO | 50% inhibition at 10% v/v, no inhibition at 2.86% v/v | Mycolicibacterium smegmatis | |
| additional information | synthesis and evaluation of a series of capuramycin analogues as WecA enzyme inhibitors. No inhibition by capuramycin, UT-01309, and SQ641 | Escherichia coli | |
| additional information | synthesis and evaluation of a series of capuramycin analogues as WecA enzyme inhibitors. No inhibition by capuramycin, UT-01309, and SQ641 | Mycolicibacterium smegmatis | |
| tunicamycin | complete inhibition at 0.02 mg/ml, 36% at 20 ng/ml | Escherichia coli | |
| tunicamycin | - |
Mycobacterium tuberculosis | |
| tunicamycin | - |
Mycolicibacterium smegmatis | |
| UT-01320 | complete inhibition at 0.02 mg/ml, 42% at 20 ng/ml | Escherichia coli | |
| UT-01320 | a selective WecA inhibitor that kills both replicating and non-replicating Mycobacterium tuberculosis cells at 0.0015-0.00258 mg/ml , respectively | Mycobacterium tuberculosis | |
| UT-01320 | - |
Mycolicibacterium smegmatis | |
| UT-03325 | 55% inhibition at 0.02 mg/ml, 30% at 20 ng/ml | Escherichia coli | |
| UT-03325 | - |
Mycolicibacterium smegmatis | |
| UT-03328 | 60.5% inhibition at 0.02 mg/ml, no inhibition at 20 ng/ml | Escherichia coli | |
| UT-03328 | - |
Mycolicibacterium smegmatis | |
| UT-03347 | 80.5% inhibition at 0.02 mg/ml, 11% at 20 ng/ml | Escherichia coli | |
| UT-03347 | - |
Mycolicibacterium smegmatis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten enzyme kinetics | Escherichia coli | |
| additional information | - |
additional information | Michaelis-Menten enzyme kinetics | Mycobacterium tuberculosis | |
| additional information | - |
additional information | Michaelis-Menten enzyme kinetics | Mycolicibacterium smegmatis |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Escherichia coli | |
| Mg2+ | required | Mycobacterium tuberculosis | |
| Mg2+ | required | Mycolicibacterium smegmatis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-N-acetyl-alpha-D-glucosamine + trans,octacis-decaprenyl phosphate | Escherichia coli | - |
UMP + N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol | - |
? | |
| UDP-N-acetyl-alpha-D-glucosamine + trans,octacis-decaprenyl phosphate | Mycobacterium tuberculosis | - |
UMP + N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol | - |
? | |
| UDP-N-acetyl-alpha-D-glucosamine + trans,octacis-decaprenyl phosphate | Mycolicibacterium smegmatis | - |
UMP + N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol | - |
? | |
| UDP-N-acetyl-alpha-D-glucosamine + trans,octacis-decaprenyl phosphate | Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 | - |
UMP + N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol | - |
? | |
| UDP-N-acetyl-alpha-D-glucosamine + trans,octacis-decaprenyl phosphate | Mycobacterium tuberculosis H37Rv | - |
UMP + N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P0AC78 | gene wecA | - |
| Mycobacterium tuberculosis | P9WMW5 | gene wecA | - |
| Mycobacterium tuberculosis H37Rv | P9WMW5 | gene wecA | - |
| Mycolicibacterium smegmatis | A0R211 | gene wecA | - |
| Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 | A0R211 | gene wecA | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| preparation of membrane fraction | Mycobacterium tuberculosis |
| preparation of membrane fraction | Mycolicibacterium smegmatis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | specificity of WecA against truncated prenyl phosphates, overview | Mycobacterium tuberculosis | ? | - |
? | |
| additional information | specificity of WecA against truncated prenyl phosphates, overview. Decaprenyl phosphate and undecaprenyl phosphate are equally effective, no activity with substrates that have shorter prenyl chains | Escherichia coli | ? | - |
? | |
| additional information | specificity of WecA against truncated prenyl phosphates, overview. Decaprenyl phosphate and undecaprenyl phosphate are equally effective, no activity with substrates that have shorter prenyl chains | Mycolicibacterium smegmatis | ? | - |
? | |
| additional information | specificity of WecA against truncated prenyl phosphates, overview. Decaprenyl phosphate and undecaprenyl phosphate are equally effective, no activity with substrates that have shorter prenyl chains | Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 | ? | - |
? | |
| additional information | specificity of WecA against truncated prenyl phosphates, overview | Mycobacterium tuberculosis H37Rv | ? | - |
? | |
| UDP-N-acetyl-alpha-D-glucosamine + trans,octacis-decaprenyl phosphate | - |
Escherichia coli | UMP + N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol | - |
? | |
| UDP-N-acetyl-alpha-D-glucosamine + trans,octacis-decaprenyl phosphate | - |
Mycobacterium tuberculosis | UMP + N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol | - |
? | |
| UDP-N-acetyl-alpha-D-glucosamine + trans,octacis-decaprenyl phosphate | - |
Mycolicibacterium smegmatis | UMP + N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol | - |
? | |
| UDP-N-acetyl-alpha-D-glucosamine + trans,octacis-decaprenyl phosphate | - |
Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 | UMP + N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol | - |
? | |
| UDP-N-acetyl-alpha-D-glucosamine + trans,octacis-decaprenyl phosphate | - |
Mycobacterium tuberculosis H37Rv | UMP + N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| polyprenyl phosphate-GlcNAc-1-phosphate transferase | - |
Escherichia coli |
| polyprenyl phosphate-GlcNAc-1-phosphate transferase | - |
Mycobacterium tuberculosis |
| polyprenyl phosphate-GlcNAc-1-phosphate transferase | - |
Mycolicibacterium smegmatis |
| WecA | - |
Escherichia coli |
| WecA | - |
Mycobacterium tuberculosis |
| WecA | - |
Mycolicibacterium smegmatis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
| 37 | - |
assay at | Mycobacterium tuberculosis |
| 37 | - |
assay at | Mycolicibacterium smegmatis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Escherichia coli |
| 8 | - |
assay at | Mycobacterium tuberculosis |
| 8 | - |
assay at | Mycolicibacterium smegmatis |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| additional information | - |
IC50 for tunicamycin is 120 ng/ml at pH 8.0 37°C | Escherichia coli | additional information | |
| additional information | - |
IC50 for tunicamycin is 590 ng/ml at pH 8.0 37°C | Mycolicibacterium smegmatis | additional information |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | inhibition of WecA will block the entire biosynthesis of essential cell wall components of Mtb in both replicating and non-replicating states | Mycobacterium tuberculosis |
| physiological function | WecA catalyzes the transformation from UDP-N-acetyl-alpha-D-glucosamine to N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol the first membrane-anchored glycophospholipid that is responsible for the biosynthesis of mycolylarabinogalactan | Mycobacterium tuberculosis |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
