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Literature summary for 2.7.8.24 extracted from

  • Solis-Oviedo, R.L.; Martinez-Morales, F.; Geiger, O.; Sohlenkamp, C.
    Functional and topological analysis of phosphatidylcholine synthase from Sinorhizobium meliloti (2012), Biochim. Biophys. Acta, 1821, 573-581.
    View publication on PubMed
Search for more literature for 2.7.8.24

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3).pLysS cells Sinorhizobium meliloti

Protein Variants

Protein Variants Comment Organism
D56A inactive Sinorhizobium meliloti
D56E inactive Sinorhizobium meliloti
D56N inactive Sinorhizobium meliloti
D59A inactive Sinorhizobium meliloti
D59E inactive Sinorhizobium meliloti
D59N inactive Sinorhizobium meliloti
D81A inactive Sinorhizobium meliloti
D81E the mutant shows 14% of wild type activity Sinorhizobium meliloti
D81N the mutant shows 21% of wild type activity Sinorhizobium meliloti
D85A inactive Sinorhizobium meliloti
D85E inactive Sinorhizobium meliloti
D85N inactive Sinorhizobium meliloti
G229D inactive Sinorhizobium meliloti
G60A inactive Sinorhizobium meliloti
G77A inactive Sinorhizobium meliloti
G77D inactive Sinorhizobium meliloti
H20A inactive Sinorhizobium meliloti
H20L the mutant shows 62% of wild type activity Sinorhizobium meliloti
L72A the mutant shows 46% of wild type activity Sinorhizobium meliloti
L72R inactive Sinorhizobium meliloti
L80A the mutant shows 85% of wild type activity Sinorhizobium meliloti
R64A the mutant shows 67% of wild type activity Sinorhizobium meliloti
T23A inactive Sinorhizobium meliloti
T23S the mutant shows 67% of wild type activity Sinorhizobium meliloti
V83A the mutant shows 39% of wild type activity Sinorhizobium meliloti
W142A inactive Sinorhizobium meliloti
Y123A the mutant shows less than 20% of wild type activity Sinorhizobium meliloti
Y86A inactive Sinorhizobium meliloti
Y89A inactive Sinorhizobium meliloti

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane a transmembrane protein Sinorhizobium meliloti 16020
-

Organism

Organism UniProt Comment Textmining
Sinorhizobium meliloti Q9KJY8
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CDP-diacylglycerol + choline
-
 Sinorhizobium meliloti CMP + phosphatidylcholine
-
 ?

Synonyms

Synonyms Comment Organism
PCS
-
 Sinorhizobium meliloti