Application | Comment | Organism |
---|---|---|
agriculture | formation of one of the two C-P bonds in the tripeptide antibiotic bialaphos, a potent herbicide isolated from Streptomyces hygroscopicus SF1293, enzyme is produced in large amounts by high producer strain HP5-29, enzyme may contribute to improvement of bialphos production | Streptomyces hygroscopicus |
Cloned (Comment) | Organism |
---|---|
mutant NP71 constructed by gene replacement technique | Streptomyces hygroscopicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | enzyme is inactive in presence of EDTA, but inhibition is completely relieved by addition of 1 mM MnSO4 or MgSO4 | Streptomyces hygroscopicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
carboxyphosphoenolpyruvate | pH 6.5, 27°C | Streptomyces hygroscopicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | increases activity, 1-10 mM MgSO4, relative activity 250% | Streptomyces hygroscopicus | |
Mn2+ | 1 mM MnSO4, relative activity 680%, 10 mM MnSO4, relative activity 600% | Streptomyces hygroscopicus | |
additional information | NaCl, KCl and NH4(SO4)2 up to 100 mM have no effect | Streptomyces hygroscopicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
2 * 32000, homodimer, SDS-PAGE | Streptomyces hygroscopicus |
56000 | - |
gel filtration | Streptomyces hygroscopicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
carboxyphosphonoenolpyruvate | Streptomyces hygroscopicus | biosynthetic pathway that leads to the tripeptide antibiotic bialaphos, formation of one of the two C-P bonds in bialaphos | (hydroxyphosphinyl)pyruvate + CO2 | - |
? | |
carboxyphosphonoenolpyruvate | Streptomyces hygroscopicus SF1293 | biosynthetic pathway that leads to the tripeptide antibiotic bialaphos, formation of one of the two C-P bonds in bialaphos | (hydroxyphosphinyl)pyruvate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces hygroscopicus | - |
HP782, HP5-29 and SF1293 mutant NP71 | - |
Streptomyces hygroscopicus | - |
SF1293 | - |
Streptomyces hygroscopicus SF1293 | - |
SF1293 | - |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces hygroscopicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Streptomyces hygroscopicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
specific activity 217.0 U/mg of protein, one unit is the amount of enzyme required to produce 1micromol of bialaphos | Streptomyces hygroscopicus |
Storage Stability | Organism |
---|---|
-20°C, 80% ammonium sulfate suspension, no loss of activity observed for 1 month | Streptomyces hygroscopicus |
4°C, 1 mg/ml in 50 mM MES buffer, pH 6.5, stable for 1 week | Streptomyces hygroscopicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carboxyphosphoenolpyruvate | - |
Streptomyces hygroscopicus | ? | - |
? | |
carboxyphosphoenolpyruvate | - |
Streptomyces hygroscopicus SF1293 | ? | - |
? | |
carboxyphosphonoenolpyruvate | catalyzes the rearrangement of the carboxyphosphonogroup to form the C-P bond of phosphinopyruvate | Streptomyces hygroscopicus | (hydroxyphosphinyl)pyruvate + CO2 | - |
? | |
carboxyphosphonoenolpyruvate | biosynthetic pathway that leads to the tripeptide antibiotic bialaphos, formation of one of the two C-P bonds in bialaphos | Streptomyces hygroscopicus | (hydroxyphosphinyl)pyruvate + CO2 | - |
? | |
carboxyphosphonoenolpyruvate | catalyzes the rearrangement of the carboxyphosphonogroup to form the C-P bond of phosphinopyruvate | Streptomyces hygroscopicus SF1293 | (hydroxyphosphinyl)pyruvate + CO2 | - |
? | |
carboxyphosphonoenolpyruvate | biosynthetic pathway that leads to the tripeptide antibiotic bialaphos, formation of one of the two C-P bonds in bialaphos | Streptomyces hygroscopicus SF1293 | (hydroxyphosphinyl)pyruvate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 32000, homodimer, SDS-PAGE | Streptomyces hygroscopicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Streptomyces hygroscopicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Streptomyces hygroscopicus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8 | stable in this range | Streptomyces hygroscopicus |