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Literature summary for 2.7.8.23 extracted from

  • Hidaka, T.; Imai, S.; Hara, O.; Anzai, H.; Murakami, T.; Nagaoka, K.; Seto, H.
    Carboxyphosphoenolpyruvate phosphonomutase, a novel enzyme catalyzing C-P bond formation (1990), J. Bacteriol., 172, 3066-3072.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
agriculture formation of one of the two C-P bonds in the tripeptide antibiotic bialaphos, a potent herbicide isolated from Streptomyces hygroscopicus SF1293, enzyme is produced in large amounts by high producer strain HP5-29, enzyme may contribute to improvement of bialphos production Streptomyces hygroscopicus

Cloned(Commentary)

Cloned (Comment) Organism
mutant NP71 constructed by gene replacement technique Streptomyces hygroscopicus

Inhibitors

Inhibitors Comment Organism Structure
EDTA enzyme is inactive in presence of EDTA, but inhibition is completely relieved by addition of 1 mM MnSO4 or MgSO4 Streptomyces hygroscopicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.25
-
carboxyphosphoenolpyruvate pH 6.5, 27°C Streptomyces hygroscopicus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ increases activity, 1-10 mM MgSO4, relative activity 250% Streptomyces hygroscopicus
Mn2+ 1 mM MnSO4, relative activity 680%, 10 mM MnSO4, relative activity 600% Streptomyces hygroscopicus
additional information NaCl, KCl and NH4(SO4)2 up to 100 mM have no effect Streptomyces hygroscopicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
2 * 32000, homodimer, SDS-PAGE Streptomyces hygroscopicus
56000
-
gel filtration Streptomyces hygroscopicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
carboxyphosphonoenolpyruvate Streptomyces hygroscopicus biosynthetic pathway that leads to the tripeptide antibiotic bialaphos, formation of one of the two C-P bonds in bialaphos (hydroxyphosphinyl)pyruvate + CO2
-
?
carboxyphosphonoenolpyruvate Streptomyces hygroscopicus SF1293 biosynthetic pathway that leads to the tripeptide antibiotic bialaphos, formation of one of the two C-P bonds in bialaphos (hydroxyphosphinyl)pyruvate + CO2
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces hygroscopicus
-
HP782, HP5-29 and SF1293 mutant NP71
-
Streptomyces hygroscopicus
-
SF1293
-
Streptomyces hygroscopicus SF1293
-
SF1293
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptomyces hygroscopicus

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
Streptomyces hygroscopicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
specific activity 217.0 U/mg of protein, one unit is the amount of enzyme required to produce 1micromol of bialaphos Streptomyces hygroscopicus

Storage Stability

Storage Stability Organism
-20°C, 80% ammonium sulfate suspension, no loss of activity observed for 1 month Streptomyces hygroscopicus
4°C, 1 mg/ml in 50 mM MES buffer, pH 6.5, stable for 1 week Streptomyces hygroscopicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxyphosphoenolpyruvate
-
Streptomyces hygroscopicus ?
-
?
carboxyphosphoenolpyruvate
-
Streptomyces hygroscopicus SF1293 ?
-
?
carboxyphosphonoenolpyruvate catalyzes the rearrangement of the carboxyphosphonogroup to form the C-P bond of phosphinopyruvate Streptomyces hygroscopicus (hydroxyphosphinyl)pyruvate + CO2
-
?
carboxyphosphonoenolpyruvate biosynthetic pathway that leads to the tripeptide antibiotic bialaphos, formation of one of the two C-P bonds in bialaphos Streptomyces hygroscopicus (hydroxyphosphinyl)pyruvate + CO2
-
?
carboxyphosphonoenolpyruvate catalyzes the rearrangement of the carboxyphosphonogroup to form the C-P bond of phosphinopyruvate Streptomyces hygroscopicus SF1293 (hydroxyphosphinyl)pyruvate + CO2
-
?
carboxyphosphonoenolpyruvate biosynthetic pathway that leads to the tripeptide antibiotic bialaphos, formation of one of the two C-P bonds in bialaphos Streptomyces hygroscopicus SF1293 (hydroxyphosphinyl)pyruvate + CO2
-
?

Subunits

Subunits Comment Organism
dimer 2 * 32000, homodimer, SDS-PAGE Streptomyces hygroscopicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
Streptomyces hygroscopicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Streptomyces hygroscopicus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 8 stable in this range Streptomyces hygroscopicus