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Literature summary for 2.7.8.2 extracted from
- Cholinephosphotransferase from mammalian sources (1992), Methods Enzymol., 209, 267-272.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| deoxycholate | stimulates at low subsolubilizing concentrations, membrane-solubilizing concentrations lead to nearly complete inactivation | Rattus norvegicus |  |
| lysolecithin | stimulates at low subsolubilizing concentrations, membrane-solubilizing concentrations lead to nearly complete inactivation | Rattus norvegicus | |
| Triton X-100 | stimulates at low subsolubilizing concentrations, membrane-solubilizing concentrations lead to nearly complete inactivation | Rattus norvegicus | |
| Tween-20 | stimulates at low subsolubilizing concentrations, membrane-solubilizing concentrations lead to nearly complete inactivation | Rattus norvegicus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | above 0.01 mM; competitive to Mg2+ or Mn2+ | Rattus norvegicus | |
| CMP | inhibition of the reaction CDP-choline + 1,2-diacylglycerol | Rattus norvegicus | |
| deoxycholate | stimulates at low subsolubilizing concentrations, membrane-solubilizing concentrations lead to nearly complete inactivation | Rattus norvegicus | |
| Triton X-100 | - |
Rattus norvegicus | |
| Tween-20 | stimulates at low subsolubilizing concentrations, membrane-solubilizing concentrations lead to nearly complete inactivation | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.18 | 0.35 | CMP | 37°C, pH 8.5 | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum | - |
Rattus norvegicus | 5783 | - |
| Golgi apparatus | - |
Rattus norvegicus | 5794 | - |
| membrane | nuclear membrane, integral membrane protein | Rattus norvegicus | 16020 | - |
| mitochondrion | - |
Rattus norvegicus | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | maximal activation at 5-10 mM | Rattus norvegicus | |
| Mn2+ | can partially replace Mg2+ in activation | Rattus norvegicus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CDP-choline + sn-1,2-diacylglycerol | Rattus norvegicus | final reaction in synthesis of phosphatidylcholine | ? | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CDP-choline + 1,2-diacylglycerol | - |
Rattus norvegicus | CMP + a phosphatidylcholine | - |
r | |
| CDP-choline + sn-1,2-diacylglycerol | final reaction in synthesis of phosphatidylcholine | Rattus norvegicus | ? | - |
r | |
| CMP + phosphatidylcholine | - |
Rattus norvegicus | CDP-choline + 1,2-diacylglycerol | - |
r | |
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