BRENDA - Enzyme Database
show all sequences of 2.7.8.19

Phosphoglucomutase in Saccharomyces cerevisiae is a cytoplasmic glycoprotein and the acceptor for a Glc-phosphotransferase

Marchase, R.B.; Bounelis, P.; Brumley, L.M.; Dey, N.; Browne, B.; Auger, D.; Fritz, T.A.; Kulesza, P.; Bedwell, D.M.; J. Biol. Chem. 268, 8341-8349 (1993)

Data extracted from this reference:

General Stability
General Stability
Organism
active fractions are frozen in a dry ice/methanol bath immediately after preparation and stored at -80C
Saccharomyces cerevisiae
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Rattus norvegicus
16020
-
membrane
membrane-associated enzyme, faces the cytosol
Saccharomyces cerevisiae
16020
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
added in the activity assay at a final concentration of 5 mM
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDP-glucose + phosphoglucomutase
Saccharomyces cerevisiae
-
?
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rattus norvegicus
-
-
-
Saccharomyces cerevisiae
-
strains NY13 and YDB135
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
GlcPTase from Saccharomyces cerevisiae recognizes and reacts with GlcPTase acceptor from Rattus norvegicus. The reciprocal experiment also results in glucose phosphate transfer demonstrating that this glycosylation reaction has been conserved during the evolution. Phosphoglucomutase from rabbit muscle can also act as an acceptor
639428
Rattus norvegicus
?
-
-
-
-
additional information
GlcPTase from Saccharomyces cerevisiae recognizes and reacts with GlcPTase acceptor from Rattus norvegicus. The reciprocal experiment also results in glucose phosphate transfer demonstrating that this glycosylation reaction has been conserved during the evolution. Phosphoglucomutase from rabbit muscle can also act as an acceptor
639428
Saccharomyces cerevisiae
?
-
-
-
-
UDP-glucose + glycoprotein D-mannose
-
639428
Rattus norvegicus
UMP + glycoprotein 6-(D-glucose-1-phospho)-D-mannose
-
639428
Rattus norvegicus
?
UDP-glucose + glycoprotein D-mannose
-
639428
Saccharomyces cerevisiae
UMP + glycoprotein 6-(D-glucose-1-phospho)-D-mannose
-
639428
Saccharomyces cerevisiae
?
UDP-glucose + phosphoglucomutase
-
639428
Saccharomyces cerevisiae
?
-
-
-
?
UDP-glucose + phosphoglucomutase
specific for UDP-glucose, the site affected in the GlcPTase reaction is not the enzymatic active site of phosphoglucomutase, underglucosylated forms of phosphoglucomutase obtained in response to heat shock and growth in media containing carbon sources other than glucose increase transfer in vitro GlcPTase assays and appear to reflect regulation of the GlcPTase activity
639428
Saccharomyces cerevisiae
?
-
-
-
?
General Stability (protein specific)
General Stability
Organism
active fractions are frozen in a dry ice/methanol bath immediately after preparation and stored at -80C
Saccharomyces cerevisiae
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Rattus norvegicus
16020
-
membrane
membrane-associated enzyme, faces the cytosol
Saccharomyces cerevisiae
16020
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
added in the activity assay at a final concentration of 5 mM
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDP-glucose + phosphoglucomutase
Saccharomyces cerevisiae
-
?
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
GlcPTase from Saccharomyces cerevisiae recognizes and reacts with GlcPTase acceptor from Rattus norvegicus. The reciprocal experiment also results in glucose phosphate transfer demonstrating that this glycosylation reaction has been conserved during the evolution. Phosphoglucomutase from rabbit muscle can also act as an acceptor
639428
Rattus norvegicus
?
-
-
-
-
additional information
GlcPTase from Saccharomyces cerevisiae recognizes and reacts with GlcPTase acceptor from Rattus norvegicus. The reciprocal experiment also results in glucose phosphate transfer demonstrating that this glycosylation reaction has been conserved during the evolution. Phosphoglucomutase from rabbit muscle can also act as an acceptor
639428
Saccharomyces cerevisiae
?
-
-
-
-
UDP-glucose + glycoprotein D-mannose
-
639428
Rattus norvegicus
UMP + glycoprotein 6-(D-glucose-1-phospho)-D-mannose
-
639428
Rattus norvegicus
?
UDP-glucose + glycoprotein D-mannose
-
639428
Saccharomyces cerevisiae
UMP + glycoprotein 6-(D-glucose-1-phospho)-D-mannose
-
639428
Saccharomyces cerevisiae
?
UDP-glucose + phosphoglucomutase
-
639428
Saccharomyces cerevisiae
?
-
-
-
?
UDP-glucose + phosphoglucomutase
specific for UDP-glucose, the site affected in the GlcPTase reaction is not the enzymatic active site of phosphoglucomutase, underglucosylated forms of phosphoglucomutase obtained in response to heat shock and growth in media containing carbon sources other than glucose increase transfer in vitro GlcPTase assays and appear to reflect regulation of the GlcPTase activity
639428
Saccharomyces cerevisiae
?
-
-
-
?
Other publictions for EC 2.7.8.19
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
639429
Dey
The glycosylation of phosphogl ...
Saccharomyces cerevisiae
J. Biol. Chem.
269
27143-27148
1994
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639428
Marchase
Phosphoglucomutase in Saccharo ...
Rattus norvegicus, Saccharomyces cerevisiae
J. Biol. Chem.
268
8341-8349
1993
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7
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639424
Satir
Parafusin, an exocytic-sensiti ...
Paramecium tetraurelia, Rattus norvegicus
J. Cell Biol.
111
901-907
1990
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4
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639427
Marchase
Resolution of phosphoglucomuta ...
Rattus norvegicus
Arch. Biochem. Biophys.
280
122-129
1990
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639425
Srisomsap
Localization of the glucose ph ...
Rattus norvegicus
J. Biol. Chem.
263
17792-17797
1988
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639426
Hiller
Glucose-1-phosphotransferase a ...
Gallus gallus, Homo sapiens
J. Biol. Chem.
262
4377-4381
1987
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639423
Koro
A UDP-glucose:glycoprotein glu ...
Gallus gallus
Cell
31
739-748
1982
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