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Literature summary for 2.7.8.17 extracted from

  • Encarnacao, M.; Kollmann, K.; Trusch, M.; Braulke, T.; Pohl, S.
    Post-translational modifications of the gamma-subunit affect intracellular trafficking and complex assembly of GlcNAc-1-phosphotransferase (2011), J. Biol. Chem., 286, 5311-5318.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.8.17

Cloned(Commentary)

Cloned (Comment) Organism
gamma subunit is over-expressed in COS-7 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
C142S mutant expressed as dimer Homo sapiens
C157S mutant expressed as dimer Homo sapiens
C157S/C245S mutant can be detected as 36 kDa monomeric form but not as a dimer Homo sapiens
C169S mutant expressed as dimer Homo sapiens
C245S mutant can be detected as 36 kDa monomeric form but not as a dimer. Mutant is localised in the Golgi apparatus, indicating that dimerization is not essential for endoplasmic reticulum exit of the gamma-subunit. Monomeric C245S mutant does not assemble with endogenous GlcNAc-1-phosphotransferase 1 subunits Homo sapiens
C84S mutant expressed as dimer Homo sapiens
C84S/C157S mutant expressed as dimer Homo sapiens
C84S/C157S/C245S mutant can be detected as 36 kDa monomeric form but not as a dimer Homo sapiens
N115Q electrophoretic migration similar to wild-type at 34 kDa, mutant sensitive to PNGase F treatment Homo sapiens
N88Q electrophoretic migration similar to wild-type at 34 kDa, mutant sensitive to PNGase F treatment Homo sapiens
N88Q/N115Q electrophoretic migration at 31 kDa, double mutant insensitive to PNGase F treatment, non-glycosylated polypeptide. Non-glycosylated gamma-subunits do not colocalize with the Golgi apparatus marker GM130 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cis-Golgi network gamma subunit is localised in the cis-Golgi apparatus Homo sapiens 5801
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein enzyme contains N-glycosylation sites at position 88 and 115. N-glycosylation is important for intracellular trafficking of the gamma subunit Homo sapiens

Subunits

Subunits Comment Organism
dimer 2 * 36000 Homo sapiens
More dimerization is important for the enzyme to assemble in high molecular mass GlcNAc-1-phosphotransferase 1 subunits Homo sapiens

Synonyms

Synonyms Comment Organism
GlcNAc-1-phosphotransferase
-
 Homo sapiens