Inhibitors | Comment | Organism | Structure |
---|---|---|---|
UDP | - |
Homo sapiens | |
UDP | 5 mM, 63% inhibition | Rattus norvegicus | |
UDPglucose | competitive with respect to UDP-GlcNAc | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.038 | - |
UDP-GlcNAc | pH 6.5, 37°C | Rattus norvegicus | |
183 | - |
methyl-alpha-D-mannoside | pH 6.5, 37°C | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
Golgi apparatus | - |
Mus musculus | 5794 | - |
Golgi apparatus | - |
Homo sapiens | 5794 | - |
Golgi apparatus | - |
Rattus norvegicus | 5794 | - |
Golgi membrane | integral membrane protein | Rattus norvegicus | 139 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens | |
Mg2+ | the divalent cation requirement is satisfied by Mn2+ better than by Mg2+ | Rattus norvegicus | |
Mn2+ | - |
Homo sapiens | |
Mn2+ | the divalent cation requirement is satisfied by Mn2+ better than by Mg2+ | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Mus musculus | - |
- |
- |
Ovis aries | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Homo sapiens | - |
BW5147 cell | - |
Mus musculus | - |
fibroblast | - |
Homo sapiens | - |
kidney | - |
Homo sapiens | - |
liver | - |
Homo sapiens | - |
liver | - |
Rattus norvegicus | - |
liver | - |
Ovis aries | - |
P388D1 cell | - |
Mus musculus | - |
peripheral blood mononuclear cell | - |
Homo sapiens | - |
placenta | - |
Homo sapiens | - |
spleen | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.000733 | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
0°C, 2 months, less than 10% loss of activity | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | - |
Homo sapiens | ? | - |
? | |
additional information | UDPglucose is also a substrate with a catalytic efficiency about 12fold worse than UDP-GlcNAc. The enzyme phosphorylates lysosomal enzymes in an in vitro assay at least 100fold more efficiently than either other glycoproteins with similar carbohydrate chains or free oligosaccharides | Rattus norvegicus | ? | - |
? | |
UDP-N-acetyl-D-glucosamine + methyl-alpha-D-mannoside | - |
Rattus norvegicus | UMP + N-acetyl-D-glucosamine-phospho-(methyl-alpha-D-mannoside) | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.5 | with Tris-HCl giving 50% more activity than sodium cacodylate | Rattus norvegicus |