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Literature summary for 2.7.8.17 extracted from

  • Waheed, A.; Hasilik, A.; von Figura, K.
    UDP-N-acetylglucosamine:lysosomal enzyme precursor N-acetylglucosamine-1-phosphotransferase. Partial purification and characterization of the rat liver Golgi enzyme (1982), J. Biol. Chem., 257, 12322-12331.
    View publication on PubMed

General Stability

General Stability Organism
treatment with papain or phospholipase C from Bacillus cereus inactivates the enzyme in a time- and dose-dependent manner Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
(Man)5GlcNAc
-
Rattus norvegicus
(Man)7GlcNAc
-
Rattus norvegicus
methyl-alpha-D-mannoside
-
Rattus norvegicus
NaCl 0.1 M, 36% inhibition. 0.5 M, 91% inhibition Rattus norvegicus
p-nitrophenyl-alpha-D-mannoside
-
Rattus norvegicus
UDPGlcNAc
-
Rattus norvegicus
UDPglucose
-
Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi apparatus
-
Rattus norvegicus 5794
-
lysosome
-
Rattus norvegicus 5764
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ can substitute for Mn2+ Rattus norvegicus
Mn2+ divalent cation required, maximal activity at 10 mM Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00000038
-
-
Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-D-glucosamine + alpha-L-fucosidase
-
Rattus norvegicus UMP + ?
-
?
UDP-N-acetyl-D-glucosamine + arylsulfatase A mature arylsulfatase A from human urine Rattus norvegicus additional information formation of GlcNAc(alpha1)phospho(6)mannose diesters in high mannose oligosaccharides in arylsulfatase A ?
UDP-N-acetyl-D-glucosamine + beta-hexosaminidase the alpha-chain of beta-hexosaminidase is a poorer acceptor than the beta-chain, and the beta chain in the B isoenzyme is a better acceptor than the beta-chain in the A isoenzyme Rattus norvegicus UMP + ?
-
?
UDP-N-acetyl-D-glucosamine + cathepsin D
-
Rattus norvegicus UMP + ?
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 22 16 h, less than 20% loss of activity Rattus norvegicus
37
-
2 h, less than 20% loss of activity Rattus norvegicus
50
-
10 min, complete loss of activity Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
6 9 about 50% of maximal activity at pH 6 and at pH 9 Rattus norvegicus