General Stability | Organism |
---|---|
treatment with papain or phospholipase C from Bacillus cereus inactivates the enzyme in a time- and dose-dependent manner | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(Man)5GlcNAc | - |
Rattus norvegicus | |
(Man)7GlcNAc | - |
Rattus norvegicus | |
methyl-alpha-D-mannoside | - |
Rattus norvegicus | |
NaCl | 0.1 M, 36% inhibition. 0.5 M, 91% inhibition | Rattus norvegicus | |
p-nitrophenyl-alpha-D-mannoside | - |
Rattus norvegicus | |
UDPGlcNAc | - |
Rattus norvegicus | |
UDPglucose | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
Golgi apparatus | - |
Rattus norvegicus | 5794 | - |
lysosome | - |
Rattus norvegicus | 5764 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | can substitute for Mn2+ | Rattus norvegicus | |
Mn2+ | divalent cation required, maximal activity at 10 mM | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00000038 | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-glucosamine + alpha-L-fucosidase | - |
Rattus norvegicus | UMP + ? | - |
? | |
UDP-N-acetyl-D-glucosamine + arylsulfatase A | mature arylsulfatase A from human urine | Rattus norvegicus | additional information | formation of GlcNAc(alpha1)phospho(6)mannose diesters in high mannose oligosaccharides in arylsulfatase A | ? | |
UDP-N-acetyl-D-glucosamine + beta-hexosaminidase | the alpha-chain of beta-hexosaminidase is a poorer acceptor than the beta-chain, and the beta chain in the B isoenzyme is a better acceptor than the beta-chain in the A isoenzyme | Rattus norvegicus | UMP + ? | - |
? | |
UDP-N-acetyl-D-glucosamine + cathepsin D | - |
Rattus norvegicus | UMP + ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 22 | 16 h, less than 20% loss of activity | Rattus norvegicus |
37 | - |
2 h, less than 20% loss of activity | Rattus norvegicus |
50 | - |
10 min, complete loss of activity | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | about 50% of maximal activity at pH 6 and at pH 9 | Rattus norvegicus |