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Literature summary for 2.7.8.15 extracted from

  • Kaushal, G.P.; Elbein, A.D.
    Properties of solubilized UDP-GlcNAc:dolichyl phosphate-GlcNAc-1-P-transferase from soybean cultured cells (1986), Plant Physiol., 82, 748-752.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
phosphatidylglycerol 2- to 3fold maximal stimulation at 0.06 mg/ml Glycine max
phosphatidylinositol stimulates Glycine max

Inhibitors

Inhibitors Comment Organism Structure
Amphomycin
-
Glycine max
Ca2+ above 2 mM Glycine max
Diumycin
-
Glycine max
GDP-mannose
-
Glycine max
Mn2+ above 2 mM Glycine max
phosphatidylcholine
-
Glycine max
phosphatidylethanolamine
-
Glycine max
phosphatidylserine
-
Glycine max
Showdomycin
-
Glycine max
tunicamycin
-
Glycine max
UDP-glucose
-
Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00042
-
UDP-GlcNAc pH 7.5, 37°C Glycine max
0.0062
-
dolichyl phosphate pH 7.5, 37°C Glycine max

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Glycine max
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1-2 mM, weak stimulation Glycine max
Mg2+ 10 mM required for optimal activity Glycine max
Mn2+ 1-2 mM, weak stimulation Glycine max

Organism

Organism UniProt Comment Textmining
Glycine max
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Glycine max
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-alpha-D-glucosamine + dolichyl phosphate
-
Glycine max UMP + N-acetyl-alpha-D-glucosaminyl-diphosphodolichol
-
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pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2 7.6
-
Glycine max