Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphatidylglycerol | activity is stimulated 5-10fold by inclusion of 0.1 mg/ml in both radiochemical and fluorescence enhancement assays | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
overexpressed in recombinant Escherichia coli JM109 | Escherichia coli |
General Stability | Organism |
---|---|
no loss of activity observed upon gel filtration, desalted enzyme is completely inactive | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
mureidomycin A | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
dodecaprenylphosphate | pH 7.5, 30°C | Escherichia coli | |
0.019 | - |
dansyl-UDP-NAc-muramoyl-pentapeptide | pH 7.5, 30°C | Escherichia coli | |
0.019 | - |
heptaprenylphosphate | pH 7.5, 30°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | can be replaced only by Mn2+ | Escherichia coli | |
additional information | no activity is observed using Ni2+, Ca2+ and Zn2+ | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | 45000 | SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-MurNAc-pentapeptide + undecaprenyl-phosphate | Escherichia coli | natural acceptor | UMP + MurNAc-pentapeptide diphosphoryl undecaprenol | - |
r | |
UDP-NAc-muramoyl-pentapeptide + acceptor | Escherichia coli | biosynthesis of peptidoglycan, first lipid intermediate | UMP + acceptor-phospho-NAc-muramoyl-pentapeptide | - |
r | |
UDP-NAc-muramoyl-pentapeptide + acceptor | Escherichia coli JM105 | biosynthesis of peptidoglycan, first lipid intermediate | UMP + acceptor-phospho-NAc-muramoyl-pentapeptide | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
wild-type JM105 | - |
Escherichia coli JM105 | - |
wild-type JM105 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
specific activity is increased 2fold by induction with isopropyl-beta-D-thiogalactopyranoside in the late logarithmic phase | Escherichia coli |
additional information | - |
0.42 units, using fluorescence enhancement assay, recombinant strain JM109 (pTrc99A), 11.9 units, recombinant strain JM109 (pBROC525), activity is expressed as increase in units of fluorescence emission per min at 30°C | Escherichia coli |
0.000276 | - |
specific activity of particulate enzyme from recombinant strain JM109 (pBROC525), in absence of exogenous lipid acceptor | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dansyl-UDP-NAc-muramoyl-pentapeptide + undecaprenyl phosphate | - |
Escherichia coli | UMP + dansyl-UDP-NAc-muramoyl-pentapeptide-diphosphoundecaprenol | - |
? | |
UDP-MurNAc(oyl-L-Ala-gamma-D-Glu-meso-2,6-diaminopimelyl-(N-dansyl)-D-Ala-D-Ala) + dodecaprenyl phosphate | dPP | Escherichia coli | UMP + MurNAc(oyl-L-Ala-gamma-D-Glu-meso-2,6-diaminopimelyl-(N-dansyl)-D-Ala-D-Ala)-diphosphododecaprenol | - |
r | |
UDP-MurNAc(oyl-L-Ala-gamma-D-Glu-meso-2,6-diaminopimelyl-(N-dansyl)-D-Ala-D-Ala) + dodecaprenyl phosphate | dPP | Escherichia coli JM105 | UMP + MurNAc(oyl-L-Ala-gamma-D-Glu-meso-2,6-diaminopimelyl-(N-dansyl)-D-Ala-D-Ala)-diphosphododecaprenol | - |
r | |
UDP-MurNAc-pentapeptide + undecaprenoid-1-ol-phosphate | - |
Escherichia coli | lipid-P-P-MurNAc-pentapeptide + UMP | - |
r | |
UDP-MurNAc-pentapeptide + undecaprenoid-1-ol-phosphate | - |
Escherichia coli JM105 | lipid-P-P-MurNAc-pentapeptide + UMP | - |
r | |
UDP-MurNAc-pentapeptide + undecaprenyl-phosphate | natural acceptor | Escherichia coli | UMP + MurNAc-pentapeptide diphosphoryl undecaprenol | - |
r | |
UDP-NAc-muramoyl-pentapeptide + acceptor | - |
Escherichia coli | UMP + acceptor-phospho-NAc-muramoyl-pentapeptide | - |
r | |
UDP-NAc-muramoyl-pentapeptide + acceptor | biosynthesis of peptidoglycan, first lipid intermediate | Escherichia coli | UMP + acceptor-phospho-NAc-muramoyl-pentapeptide | - |
r | |
UDP-NAc-muramoyl-pentapeptide + acceptor | - |
Escherichia coli JM105 | UMP + acceptor-phospho-NAc-muramoyl-pentapeptide | - |
r | |
UDP-NAc-muramoyl-pentapeptide + acceptor | biosynthesis of peptidoglycan, first lipid intermediate | Escherichia coli JM105 | UMP + acceptor-phospho-NAc-muramoyl-pentapeptide | - |
r | |
UDP-NAc-muramoyl-pentapeptide + dodecaprenyl-phosphate | dodecaprenyl phosphate is a more efficient substrate than heptaprenyl-phosphate, enzyme is selective for larger substrate which is closer in chain length to the natural substrate, undecaprenyl-phosphate | Escherichia coli | UMP + acceptor-phospho-NAc-muramoyl-pentapeptide | - |
r | |
UDP-NAc-muramoyl-pentapeptide + dodecaprenyl-phosphate | dodecaprenyl phosphate is a more efficient substrate than heptaprenyl-phosphate, enzyme is selective for larger substrate which is closer in chain length to the natural substrate, undecaprenyl-phosphate | Escherichia coli JM105 | UMP + acceptor-phospho-NAc-muramoyl-pentapeptide | - |
r | |
UDP-NAc-muramoyl-pentapeptide + heptaprenyl-phosphate | - |
Escherichia coli | UMP + acceptor-phospho-NAc-muramoyl-pentapeptide | - |
r |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000036 | - |
mureidomycin A | pH 7.5, 30°C | Escherichia coli |