General Stability | Organism |
---|---|
only 2.5% of the activity can be recovered after dialysis | Staphylococcus aureus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Staphylococcus aureus | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | 200000 | second peak of exchange activity, low molecular weight form, gel filtration | Staphylococcus aureus |
2000000 | - |
major peak of activity, high molecular weight form, gel filtration | Staphylococcus aureus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-NAc-muramoyl-pentapeptide + acceptor | Staphylococcus aureus | initial stage in peptidoglycan synthesis, key role in selecting analogs of UDP-MurNAc-Ala-gammaDGlu-Lys-DAla-DAla, membrane associated reaction of cell wall biosynthesis | UMP + acceptor-phospho-NAc-muramoyl-pentapeptide | - |
r | |
UDP-NAc-muramoyl-pentapeptide + acceptor | Staphylococcus aureus | biosynthesis of peptidoglycan, first lipid intermediate | UMP + acceptor-phospho-NAc-muramoyl-pentapeptide | - |
r | |
UDP-NAc-muramoyl-pentapeptide + acceptor | Staphylococcus aureus Copenhagen | initial stage in peptidoglycan synthesis, key role in selecting analogs of UDP-MurNAc-Ala-gammaDGlu-Lys-DAla-DAla, membrane associated reaction of cell wall biosynthesis | UMP + acceptor-phospho-NAc-muramoyl-pentapeptide | - |
r | |
UDP-NAc-muramoyl-pentapeptide + acceptor | Staphylococcus aureus Copenhagen | biosynthesis of peptidoglycan, first lipid intermediate | UMP + acceptor-phospho-NAc-muramoyl-pentapeptide | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | - |
Copenhagen | - |
Staphylococcus aureus Copenhagen | - |
Copenhagen | - |
Purification (Comment) | Organism |
---|---|
low molecular weight form | Staphylococcus aureus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
specific activity of the enzyme increases 4fold by late-logarithmic phase | Staphylococcus aureus |
Storage Stability | Organism |
---|---|
-196°C, retains complete activity for at least 2 months | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetylmuramoyl-pentapeptide + [2H]UMP | - |
Staphylococcus aureus | UMP + [2H]UDP-NAc-muramoyl-pentapeptide | - |
r | |
UDP-N-acetylmuramoyl-pentapeptide + [2H]UMP | - |
Staphylococcus aureus Copenhagen | UMP + [2H]UDP-NAc-muramoyl-pentapeptide | - |
r | |
UDP-NAc-muramoyl-pentapeptide + acceptor | - |
Staphylococcus aureus | UMP + acceptor-phospho-NAc-muramoyl-pentapeptide | - |
r | |
UDP-NAc-muramoyl-pentapeptide + acceptor | initial stage in peptidoglycan synthesis, key role in selecting analogs of UDP-MurNAc-Ala-gammaDGlu-Lys-DAla-DAla, membrane associated reaction of cell wall biosynthesis | Staphylococcus aureus | UMP + acceptor-phospho-NAc-muramoyl-pentapeptide | - |
r | |
UDP-NAc-muramoyl-pentapeptide + acceptor | biosynthesis of peptidoglycan, first lipid intermediate | Staphylococcus aureus | UMP + acceptor-phospho-NAc-muramoyl-pentapeptide | - |
r | |
UDP-NAc-muramoyl-pentapeptide + acceptor | - |
Staphylococcus aureus Copenhagen | UMP + acceptor-phospho-NAc-muramoyl-pentapeptide | - |
r | |
UDP-NAc-muramoyl-pentapeptide + acceptor | initial stage in peptidoglycan synthesis, key role in selecting analogs of UDP-MurNAc-Ala-gammaDGlu-Lys-DAla-DAla, membrane associated reaction of cell wall biosynthesis | Staphylococcus aureus Copenhagen | UMP + acceptor-phospho-NAc-muramoyl-pentapeptide | - |
r | |
UDP-NAc-muramoyl-pentapeptide + acceptor | biosynthesis of peptidoglycan, first lipid intermediate | Staphylococcus aureus Copenhagen | UMP + acceptor-phospho-NAc-muramoyl-pentapeptide | - |
r |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Staphylococcus aureus | electrofocusing in a pH gradient 3-8 containing 8 M urea | - |
4.9 |