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Literature summary for 2.7.8.12 extracted from
- The synthesis of teichoic acids. I. POLYGLYCEROPHOSPHATE (1964), J. Biol. Chem., 239, 3168-3177.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| bacitracin | 94% of maximal activity | Bacillus licheniformis |  |
| Cetylpyridinium chloride | 47% of maximal activity | Bacillus licheniformis | |
| Crystal violet | 22% of maximal activity | Bacillus licheniformis | |
| Crystal violet | 22% of maximal activity | Bacillus subtilis | |
| CTP | excess concentration causes slight inhibition | Bacillus licheniformis | |
| Mn2+ | slight stimulation, in presence of Mg2+ inhibition | Bacillus licheniformis | |
| novobiocin | complete inhibition | Bacillus licheniformis | |
| penicillin | 27% of maximal activity | Bacillus licheniformis | |
| Ristocetin | 90% of maximal activity | Bacillus licheniformis | |
| spermidine | 78% of maximal activity | Bacillus licheniformis | |
| streptomycin | 89% of maximal activity | Bacillus licheniformis | |
| Vancomycin | 31% of maximal activity | Bacillus licheniformis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00083 | - |
CDP-glycerol | - |
Bacillus licheniformis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Bacillus subtilis | 16020 | - |
| membrane | protoplast membrane | Bacillus licheniformis | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 0.01 M: optimal, requirement for a divalent cation | Bacillus licheniformis | |
| Mg2+ | 0.04 M: optimal, requirement for a divalent cation | Bacillus licheniformis | |
| Mn2+ | slight stimulation, in presence of Mg2+ inhibition | Bacillus licheniformis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CDP-glycerol + (glycerophosphate)n | Bacillus subtilis | involved in teichoic acid synthesis | CMP + (glycerophosphate)n+1 | - |
? | |
| CDP-glycerol + (glycerophosphate)n | Bacillus licheniformis | involved in teichoic acid synthesis | CMP + (glycerophosphate)n+1 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus licheniformis | - |
ATCC 9945 | - |
| Bacillus subtilis | - |
NCTC 3610 | - |
| Bacillus subtilis | - |
ATCC 6051 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ATCC 6051, partial | Bacillus subtilis |
| ATCC 9945, partial | Bacillus licheniformis |
| NCTC 3610, partial | Bacillus subtilis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| frozen, no essential loss of activity within several weeks | Bacillus subtilis |
| frozen, no essential loss of activity within several weeks | Bacillus licheniformis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CDP-glycerol + (glycerophosphate)n | - |
Bacillus subtilis | CMP + (glycerophosphate)n+1 | crude extract of protoplast membrane preparation, CMP postulated | ? | |
| CDP-glycerol + (glycerophosphate)n | - |
Bacillus licheniformis | CMP + (glycerophosphate)n+1 | crude extract of protoplast membrane preparation, CMP postulated | ? | |
| CDP-glycerol + (glycerophosphate)n | involved in teichoic acid synthesis | Bacillus subtilis | CMP + (glycerophosphate)n+1 | - |
? | |
| CDP-glycerol + (glycerophosphate)n | involved in teichoic acid synthesis | Bacillus licheniformis | CMP + (glycerophosphate)n+1 | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Bacillus subtilis |
| 37 | - |
assay at | Bacillus licheniformis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
and pH 9.0, 2 maxima | Bacillus licheniformis |
| 9 | - |
and pH 7.0, 2 maxima | Bacillus licheniformis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 9.5 | 50% of maximal activity at pH 6.5, 25% of maximal activity at pH 8.0, 70% of maximal activity at pH 9.5 | Bacillus licheniformis |
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