Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Triton X-100 | - |
Rattus norvegicus | |
Triton X-100 | reaction is dependent on for maximum activity | Saccharomyces cerevisiae |
Cloned (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
disruption of the PIS locus in the genome is lethal | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
H114Q | oligonucleotide-mediated site-directed mutagenesis, substitution of amino acid at position 114 from His (CAC) + Gln (CAA) results in a 200fold increase in Km of the enzyme for myo-inositol, making cells auxotrophic for myo-inositol | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
CDP-diacylglycerol | ? | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Rattus norvegicus | - |
- |
microsome | - |
Saccharomyces cerevisiae | - |
- |
mitochondrial membrane | outer mitochondrial membrane | Saccharomyces cerevisiae | 31966 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Rattus norvegicus | |
Mg2+ | - |
Saccharomyces cerevisiae | |
Mn2+ | - |
Rattus norvegicus | |
Mn2+ | - |
Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23610 | - |
primary translate deduced from the encoding gene, PIS | Rattus norvegicus |
24820 | - |
calculated molecular mass from the cloned PI synthase gene | Saccharomyces cerevisiae |
34000 | - |
- |
Rattus norvegicus |
34000 | - |
SDS-PAGE | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CDP-diacylglycerol + myo-inositol | Rattus norvegicus | - |
CMP + phosphatidyl-1D-myo-inositol | - |
r | |
CDP-diacylglycerol + myo-inositol | Saccharomyces cerevisiae | - |
CMP + phosphatidyl-1D-myo-inositol | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
no activity in Escherichia coli | - |
- |
- |
Rattus norvegicus | - |
rat | - |
Saccharomyces cerevisiae | - |
yeast | - |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CDP-diacylglycerol + myo-inositol | - |
Rattus norvegicus | CMP + phosphatidyl-1D-myo-inositol | - |
r | |
CDP-diacylglycerol + myo-inositol | - |
Saccharomyces cerevisiae | CMP + phosphatidyl-1D-myo-inositol | - |
r |
Synonyms | Comment | Organism |
---|---|---|
PI synthase | - |
Saccharomyces cerevisiae |