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Literature summary for 2.7.8.11 extracted from
- Phosphatidylinositol biosynthesis in Saccharomyces cerevisiae: purification and properties of microsome-associated phosphatidylinositol synthase (1983), J. Bacteriol., 154, 304-311.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Triton X-100 | reaction is dependent on for maximum activity | Saccharomyces cerevisiae |  |
General Stability
| General Stability | Organism |
|---|---|
| activity is stable for at least 4 cycles of freezing and thawing | Saccharomyces cerevisiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | - |
Saccharomyces cerevisiae | |
| Hg2+ | - |
Saccharomyces cerevisiae | |
| additional information | inactivated by thioreactive reagents | Saccharomyces cerevisiae | |
| p-chloromercuribenzene sulfonate | - |
Saccharomyces cerevisiae | |
| Zn2+ | - |
Saccharomyces cerevisiae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Saccharomyces cerevisiae | 16020 | - |
| microsome | - |
Saccharomyces cerevisiae | - |
- |
| mitochondrion | - |
Saccharomyces cerevisiae | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | either Mg2+ or Mn2+ ions are necessary | Saccharomyces cerevisiae | |
| Mn2+ | absolute requirement, reaction is dependent on for maximum activity | Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
? * 34000, SDS-PAGE | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CDP-diacylglycerol + myo-inositol | Saccharomyces cerevisiae | formation of membrane phospholipids essential for the growth | CMP + phosphatidyl-1D-myo-inositol | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
yeast | - |
| Saccharomyces cerevisiae | - |
wild-type strain S288C(alpha gal2) | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.8 | - |
- |
Saccharomyces cerevisiae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, purified enzyme is completely stable for at least 6 months | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CDP-diacylglycerol + myo-inositol | - |
Saccharomyces cerevisiae | CMP + phosphatidyl-1D-myo-inositol | - |
r | |
| CDP-diacylglycerol + myo-inositol | formation of membrane phospholipids essential for the growth | Saccharomyces cerevisiae | CMP + phosphatidyl-1D-myo-inositol | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | ? * 34000, SDS-PAGE | Saccharomyces cerevisiae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PI synthase | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Saccharomyces cerevisiae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
reasonably stable up to, essentially 100% stable for at least 10 min at temperatures ranging from 5 to 50°C, inactivation of about 35% at 60°C and 100% at 70°C | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Saccharomyces cerevisiae |
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