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Literature summary for 2.7.7.92 extracted from
- Synthesis of CMP-deaminoneuraminic acid (CMP-KDN) using the CTP:CMP-3-deoxynonulosonate cytidylyltransferase from rainbow trout testis: Identification and characterization of a CMP-KDN synthetase (1993), J. Biol. Chem., 268, 2640-2648.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.7 | - |
CTP | cosubstrate N-glycolylneuraminic acid, pH 9, 25°C | Oncorhynchus mykiss |  |
| 2.6 | - |
CTP | cosubstrate 3-deoxy-D-glycero-D-galacto-nononate, pH 9, 25°C | Oncorhynchus mykiss | |
| 2.7 | - |
CTP | cosubstrate N-acetylneuraminic acid, pH 9, 25°C | Oncorhynchus mykiss | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | or Mn 2+, required. 25 mg2+ stimulates reaction with 3-deoxy-D-glycero-D-galacto-nononate more than 10fold | Oncorhynchus mykiss | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 260000 | - |
gel filtration | Oncorhynchus mykiss |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CTP + 3-deoxy-D-glycero-D-galacto-nononate | Oncorhynchus mykiss | preferred substrate | diphosphate + CMP-3-deoxy-D-glycero-D-galacto-nononate | - |
? | |
| CTP + N-acetylneuraminic acid | Oncorhynchus mykiss | - |
diphosphate + CMP-N-acetylneuraminic acid | - |
? | |
| CTP + N-glycolylneuraminic acid | Oncorhynchus mykiss | - |
diphosphate + CMP-N-glycolylneuraminic acid | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oncorhynchus mykiss | Q90WG6 | cf. EC 2.7.7.43 | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| sperm | expression of CMP-KDN synthetase is temporally correlated with development and parallels the developmental expression of (KDN)GM3 in sperm | Oncorhynchus mykiss | - |
| testis | - |
Oncorhynchus mykiss | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CTP + 3-deoxy-D-glycero-D-galacto-nononate | preferred substrate | Oncorhynchus mykiss | diphosphate + CMP-3-deoxy-D-glycero-D-galacto-nononate | - |
? | |
| CTP + N-acetylneuraminic acid | - |
Oncorhynchus mykiss | diphosphate + CMP-N-acetylneuraminic acid | - |
? | |
| CTP + N-glycolylneuraminic acid | - |
Oncorhynchus mykiss | diphosphate + CMP-N-glycolylneuraminic acid | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CMP-KDN synthetase | - |
Oncorhynchus mykiss |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
- |
Oncorhynchus mykiss |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | 10 | - |
Oncorhynchus mykiss |
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