Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Streptomyces hygroscopicus subsp. jinggangensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | able to rescue the activity of the EDTA-treated enzyme | Streptomyces hygroscopicus subsp. jinggangensis | |
Mg2+ | able to rescue the activity of the EDTA-treated enzyme | Streptomyces hygroscopicus subsp. jinggangensis | |
Mn2+ | able to rescue the activity of the EDTA-treated enzyme | Streptomyces hygroscopicus subsp. jinggangensis | |
additional information | divalent cation Mg2+, Mn2+, or Co2+ required, Fe2+, Cu2+, Ca2+, Zn2+, and Ni2+ are inactive | Streptomyces hygroscopicus subsp. jinggangensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
x * 45000, SDS-PAGE | Streptomyces hygroscopicus subsp. jinggangensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + valienol 1-phosphate | Streptomyces hygroscopicus subsp. jinggangensis | - |
diphosphate + GDP-valienol | - |
? | |
GTP + valienol 1-phosphate | Streptomyces hygroscopicus subsp. jinggangensis 5008 | - |
diphosphate + GDP-valienol | - |
? | |
additional information | Streptomyces hygroscopicus subsp. jinggangensis | ValB efficiently catalyzes the conversion of valienol 1-phosphate to its nucleotidyl diphosphate derivatives, other unsaturated carbasugar phosphates are poor substrates. Purine-based nucleotidyltriphosphates ATP and GTP are more efficiently used as nucleotidyl donor than the pyrimidine-based CTP, dTTP, and UTP | ? | - |
? | |
additional information | Streptomyces hygroscopicus subsp. jinggangensis 5008 | ValB efficiently catalyzes the conversion of valienol 1-phosphate to its nucleotidyl diphosphate derivatives, other unsaturated carbasugar phosphates are poor substrates. Purine-based nucleotidyltriphosphates ATP and GTP are more efficiently used as nucleotidyl donor than the pyrimidine-based CTP, dTTP, and UTP | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces hygroscopicus subsp. jinggangensis | Q3T6E3 | - |
- |
Streptomyces hygroscopicus subsp. jinggangensis 5008 | Q3T6E3 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-glucose 1-phosphate | - |
Streptomyces hygroscopicus subsp. jinggangensis | diphosphate + GDP-D-glucose | - |
? | |
ATP + D-glucose 1-phosphate | - |
Streptomyces hygroscopicus subsp. jinggangensis 5008 | diphosphate + GDP-D-glucose | - |
? | |
CTP + valienol 1-phosphate | - |
Streptomyces hygroscopicus subsp. jinggangensis | diphosphate + CDP-valienol | - |
? | |
CTP + valienol 1-phosphate | - |
Streptomyces hygroscopicus subsp. jinggangensis 5008 | diphosphate + CDP-valienol | - |
? | |
GTP + valienol 1-phosphate | - |
Streptomyces hygroscopicus subsp. jinggangensis | diphosphate + GDP-valienol | - |
? | |
GTP + valienol 1-phosphate | - |
Streptomyces hygroscopicus subsp. jinggangensis 5008 | diphosphate + GDP-valienol | - |
? | |
additional information | ValB efficiently catalyzes the conversion of valienol 1-phosphate to its nucleotidyl diphosphate derivatives, other unsaturated carbasugar phosphates are poor substrates. Purine-based nucleotidyltriphosphates ATP and GTP are more efficiently used as nucleotidyl donor than the pyrimidine-based CTP, dTTP, and UTP | Streptomyces hygroscopicus subsp. jinggangensis | ? | - |
? | |
additional information | ValB efficiently catalyzes the conversion of valienol 1-phosphate to its nucleotidyl diphosphate derivatives, other unsaturated carbasugar phosphates are poor substrates. Purine-based nucleotidyltriphosphates ATP and GTP are more efficiently used as nucleotidyl donor than the pyrimidine-based CTP, dTTP, and UTP | Streptomyces hygroscopicus subsp. jinggangensis 5008 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45000, SDS-PAGE | Streptomyces hygroscopicus subsp. jinggangensis |
Synonyms | Comment | Organism |
---|---|---|
ValB | - |
Streptomyces hygroscopicus subsp. jinggangensis |
General Information | Comment | Organism |
---|---|---|
physiological function | inactivation of ValB abolishes production of validamycin A and validoxylamine A | Streptomyces hygroscopicus subsp. jinggangensis |