Literature summary for 2.7.7.9 extracted from

Fuehring, J.I.; Cramer, J.T.; Schneider, J.; Baruch, P.; Gerardy-Schahn, R.; Fedorov, R.
A quaternary mechanism enables the complex biological functions of octameric human UDP-glucose pyrophosphorylase, a key enzyme in cell metabolism (2015), Sci. Rep., 5, 9618.

Crystallization (Commentary)

Crystallization (Comment)	Organism
in the D4 octameric structure of the hUGP1-UDP-Glc complex, all subunits have the same overall conformation. The transition of the UGP octamer between the apo- and the product-bound forms is in agreement with the Monod-Wyman-Changeux symmetry model. oligomerzation facilitates an intermolecular stabilization of the sugar moiety in the active site (interlock mechanism), enhances protein stability, enables mild positive cooperativity observed for the octameric wild-type UGP1 towards diphosphate in the reverse reaction, and may allow regulation of the UGP octamer by modification of a single subunit	Homo sapiens

Crystallization (Comment)

Organism

in the D4 octameric structure of the hUGP1-UDP-Glc complex, all subunits have the same overall conformation. The transition of the UGP octamer between the apo- and the product-bound forms is in agreement with the Monod-Wyman-Changeux symmetry model. oligomerzation facilitates an intermolecular stabilization of the sugar moiety in the active site (interlock mechanism), enhances protein stability, enables mild positive cooperativity observed for the octameric wild-type UGP1 towards diphosphate in the reverse reaction, and may allow regulation of the UGP octamer by modification of a single subunit

Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
D253L	0.063% of wild-type activity	Homo sapiens
G115D	0.004% of wild-type activity	Homo sapiens
G116A	0.067% of wild-type activity	Homo sapiens
G222A	insoluble protein	Homo sapiens
H223L	insoluble protein	Homo sapiens
K127A	0.150% of wild-type activity	Homo sapiens
K396A	0.041% of wild-type activity	Homo sapiens
L113G	insoluble protein	Homo sapiens
N251L	0.011% of wild-type activity	Homo sapiens
N328L	insoluble protein	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q16851	-	-

Synonyms

Synonyms	Comment	Organism
UPD1	splicing variant	Homo sapiens

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Release 2023.1 (January 2023)