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Literature summary for 2.7.7.9 extracted from

  • Kawano, Y.; Sekine, M.; Ihara, M.
    Identification and characterization of UDP-glucose pyrophosphorylase in cyanobacteria Anabaena sp. PCC 7120 (2014), J. Biosci. Bioeng., 117, 531-538.
    View publication on PubMed
Search for more literature for 2.7.7.9

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Nostoc sp.

Inhibitors

Inhibitors Comment Organism Structure
ADP in the presence of ATP or ADP, the activity decreases to about 60% Nostoc sp.
ATP in the presence of ATP or ADP, the activity decreases to about 60% Nostoc sp.
additional information not inhibitory: 1 mM 2-oxoglutaric acid, 3-phosphoglyceric acid, and 5-aminolevulinic acid Nostoc sp.
NAD+ presence of either NAD or NADP results in a decrease of activity to about 80% or 50%, respectively Nostoc sp.
NADP+ presence of either NAD or NADP results in a decrease of activity to about 80% or 50%, respectively Nostoc sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0424
-
 UTP pH 7.5, 37°C Nostoc sp.
0.376
-
 diphosphate pH 7.5, 37°C Nostoc sp.
0.742
-
 alpha-D-glucose 1-phosphate pH 7.5, 37°C Nostoc sp.
3.04
-
 UDP-glucose pH 7.5, 37°C Nostoc sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ absolutely required Nostoc sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32600
-
-
 Nostoc sp.
34000
-
-
 Nostoc sp.

Organism

Organism UniProt Comment Textmining
Nostoc sp. Q8YS18
-
-
Nostoc sp. PCC 7120 Q8YS18
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 as the enzyme concentration increases from 0.0125 mM to 0.6 mM, the specific activity also increases 4fold within this range and is constant at higher concentrations Nostoc sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + UDP-glucose
-
 Nostoc sp. UTP + alpha-D-glucose 1-phosphate
-
 r
diphosphate + UDP-glucose
-
 Nostoc sp. PCC 7120 UTP + alpha-D-glucose 1-phosphate
-
 r
additional information no substrates: ATP, GTP, CTP, dTTP Nostoc sp. ?
-
 ?
additional information no substrates: ATP, GTP, CTP, dTTP Nostoc sp. PCC 7120 ?
-
 ?
UTP + alpha-D-glucose 1-phosphate enzyme is highly specific for both UTP and glucose 1-phosphate Nostoc sp. diphosphate + UDP-glucose
-
 r
UTP + alpha-D-glucose 1-phosphate enzyme is highly specific for both UTP and glucose 1-phosphate Nostoc sp. PCC 7120 diphosphate + UDP-glucose
-
 r

Subunits

Subunits Comment Organism
? x * 32600, calculated, x * 34000, SDS-PAGE of His-tagged protein Nostoc sp.

Synonyms

Synonyms Comment Organism
All3274
-
 Nostoc sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.6
-
 UTP pH 7.5, 37°C Nostoc sp.
6.35
-
 diphosphate pH 7.5, 37°C Nostoc sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.81
-
 alpha-D-glucose 1-phosphate pH 7.5, 37°C Nostoc sp.
2.09
-
 UDP-glucose pH 7.5, 37°C Nostoc sp.
14.2
-
 UTP pH 7.5, 37°C Nostoc sp.
15.1
-
 diphosphate pH 7.5, 37°C Nostoc sp.