Cloned (Comment) | Organism |
---|---|
His-tagged version expressed in Escherichia coli BL21 (DE3) | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
diphosphate | pyrophosphorolysis | Arabidopsis thaliana | |
0.04 | - |
UDP-glucose | pyrophosphorolysis | Arabidopsis thaliana | |
0.06 | - |
UDP-glucose | pyrophosphorolysis | Arabidopsis thaliana | |
0.07 | - |
diphosphate | pyrophosphorolysis | Arabidopsis thaliana | |
0.08 | - |
UTP | UDP-glucose synthesis | Arabidopsis thaliana | |
0.14 | - |
alpha-D-glucose 1-phosphate | UDP-glucose synthesis | Arabidopsis thaliana | |
0.17 | - |
UTP | UDP-glucose synthesis | Arabidopsis thaliana | |
0.36 | - |
alpha-D-glucose 1-phosphate | UDP-glucose synthesis | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
53000, native PAGE and SDS-PAGE, calculated without His-tag: 51600 Da | Arabidopsis thaliana |
53000 | - |
53000, native PAGE and SDS-PAGE, calculated without His-tag: 51700 Da | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | P57751 | - |
- |
Arabidopsis thaliana | Q9M9P3 | - |
- |
Purification (Comment) | Organism |
---|---|
immobilized metal ion adsorption chromatography | Arabidopsis thaliana |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
340 | - |
UDP-glucose synthesis | Arabidopsis thaliana |
385 | - |
UDP-glucose synthesis | Arabidopsis thaliana |
510 | - |
pyrophosphorolysis | Arabidopsis thaliana |
715 | - |
pyrophosphorolysis | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UTP + alpha-D-glucose 1-phosphate | - |
Arabidopsis thaliana | UDP-glucose + diphosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 53000, native PAGE and SDS-PAGE, calculated without His-tag: 51600 Da | Arabidopsis thaliana |
monomer | 53000, native PAGE and SDS-PAGE, calculated without His-tag: 51700 Da | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
UDP-glucose pyrophosphorylase | - |
Arabidopsis thaliana |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Arabidopsis thaliana | calculated for recombinant protein | - |
6.1 |
Arabidopsis thaliana | calculated for recombinant protein | - |
6.14 |